Gene description for MFN1
Gene name mitofusin 1
Gene symbol MFN1
Other names/aliases hfzo1
hfzo2
Species Homo sapiens
 Database cross references - MFN1
ExoCarta ExoCarta_55669
Vesiclepedia VP_55669
Entrez Gene 55669
HGNC 18262
MIM 608506
UniProt Q8IWA4  
 MFN1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for MFN1
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IDA
    GTPase activity GO:0003924 IMP
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
    identical protein binding GO:0042802 IPI
    membrane scission GTPase motor activity GO:1990606 IEA
Biological Process
    mitochondrial fusion GO:0008053 IBA
    mitochondrial fusion GO:0008053 IDA
    mitochondrial fusion GO:0008053 IMP
    positive regulation of mitochondrial membrane potential GO:0010918 IEA
    GTP metabolic process GO:0046039 IDA
    mitochondrion localization GO:0051646 IBA
    mitochondrion localization GO:0051646 IMP
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrial outer membrane GO:0005741 IBA
    mitochondrial outer membrane GO:0005741 IDA
    mitochondrial outer membrane GO:0005741 TAS
    membrane GO:0016020 IDA
    outer mitochondrial membrane protein complex GO:0098799 IDA
 Experiment description of studies that identified MFN1 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MFN1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MIEF1  
Affinity Capture-Western Homo sapiens
2 ANLN 54443
Affinity Capture-MS Homo sapiens
3 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
4 MAGEA8  
Affinity Capture-MS Homo sapiens
5 MTCH1 23787
Proximity Label-MS Homo sapiens
6 GPR17 2840
Affinity Capture-MS Homo sapiens
7 KIF14 9928
Affinity Capture-MS Homo sapiens
8 TOMM22 56993
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 SYAP1 94056
Affinity Capture-MS Homo sapiens
10 HCST  
Affinity Capture-MS Homo sapiens
11 SLC25A38  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
12 NPTN 27020
Affinity Capture-MS Homo sapiens
13 EDEM1  
Affinity Capture-MS Homo sapiens
14 PARK2  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
15 PLK2  
Affinity Capture-MS Homo sapiens
16 FUNDC2 65991
Affinity Capture-MS Homo sapiens
17 SIRT1  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
18 ILF2 3608
Co-fractionation Homo sapiens
19 FLNC 2318
Affinity Capture-MS Homo sapiens
20 PPP4R1L  
Affinity Capture-MS Homo sapiens
21 CCNB1 891
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
22 NTRK1 4914
Affinity Capture-MS Homo sapiens
23 KAT5  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
24 FAF2 23197
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 SERBP1 26135
Affinity Capture-MS Homo sapiens
26 Park2  
Biochemical Activity Rattus norvegicus
27 MFN2 9927
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 KRAS 3845
Synthetic Lethality Homo sapiens
29 PSMC6 5706
Affinity Capture-MS Homo sapiens
30 MARCH5  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
31 AKAP1 8165
Proximity Label-MS Homo sapiens
32 EP300 2033
Affinity Capture-MS Homo sapiens
33 SLC25A46 91137
Proximity Label-MS Homo sapiens
View the network image/svg+xml



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