Gene description for DNAJA4
Gene name DnaJ (Hsp40) homolog, subfamily A, member 4
Gene symbol DNAJA4
Other names/aliases MST104
MSTP104
PRO1472
Species Homo sapiens
 Database cross references - DNAJA4
ExoCarta ExoCarta_55466
Vesiclepedia VP_55466
Entrez Gene 55466
HGNC 14885
UniProt Q8WW22  
 DNAJA4 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for DNAJA4
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    Hsp70 protein binding GO:0030544 IEA
    metal ion binding GO:0046872 IEA
    unfolded protein binding GO:0051082 IDA
    protein-folding chaperone binding GO:0051087 IBA
    protein-folding chaperone binding GO:0051087 IPI
Biological Process
    response to heat GO:0009408 IEA
    negative regulation of endothelial cell migration GO:0010596 IMP
    positive regulation of gene expression GO:0010628 IMP
    protein refolding GO:0042026 IBA
    protein refolding GO:0042026 IDA
    negative regulation of inclusion body assembly GO:0090084 IDA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    membrane GO:0016020 IDA
 Experiment description of studies that identified DNAJA4 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for DNAJA4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 STUB1 10273
Affinity Capture-MS Homo sapiens
2 DNAJC7 7266
Affinity Capture-MS Homo sapiens
3 ENDOG  
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
4 AHSA1 10598
Co-fractionation Homo sapiens
5 HSPA6 3310
Affinity Capture-Western Homo sapiens
6 ATP5H 10476
Cross-Linking-MS (XL-MS) Homo sapiens
7 TUBA1A 7846
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
8 MLF2 8079
Affinity Capture-MS Homo sapiens
9 TIMM50 92609
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
10 TUBB 203068
Affinity Capture-MS Homo sapiens
11 CCT3 7203
Co-fractionation Homo sapiens
12 TUBB6 84617
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 Tubb5 22154
Affinity Capture-MS Mus musculus
14 PRPS1 5631
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
15 HSPA8 3312
Co-fractionation Homo sapiens
16 XPO1 7514
Affinity Capture-MS Homo sapiens
17 EIF3I 8668
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 SH3BP4 23677
Two-hybrid Homo sapiens
19 SUGT1 10910
Affinity Capture-MS Homo sapiens
20 PSMD11 5717
Co-fractionation Homo sapiens
21 DNAJA2 10294
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 CCT6A 908
Co-fractionation Homo sapiens
23 BANP  
Two-hybrid Homo sapiens
24 CCT2 10576
Co-fractionation Homo sapiens
25 ACTC1 70
Proximity Label-MS Homo sapiens
26 BAG2 9532
Affinity Capture-MS Homo sapiens
27 UBC 7316
Affinity Capture-MS Homo sapiens
28 CCT4 10575
Co-fractionation Homo sapiens
29 GRPEL1 80273
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
30 CCT6B 10693
Co-fractionation Homo sapiens
31 DNAJC5 80331
Cross-Linking-MS (XL-MS) Homo sapiens
32 FKBP8 23770
Affinity Capture-MS Homo sapiens
33 TCTN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 CXorf57  
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
35 KPNA2 3838
Co-fractionation Homo sapiens
36 RIPK4  
Affinity Capture-MS Homo sapiens
37 TUBB4A 10382
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
38 L3MBTL1  
Affinity Capture-MS Homo sapiens
39 KIAA1161 57462
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 HSPA5 3309
Co-fractionation Homo sapiens
41 BAG6 7917
Affinity Capture-MS Homo sapiens
42 BAG5 9529
Affinity Capture-MS Homo sapiens
43 SGTB  
Affinity Capture-MS Homo sapiens
44 MLLT11  
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
45 PSMC5 5705
Co-fractionation Homo sapiens
46 CAD 790
Co-fractionation Homo sapiens
47 GPR45  
Affinity Capture-MS Homo sapiens
48 SORT1 6272
Affinity Capture-MS Homo sapiens
49 TIPRL 261726
Affinity Capture-MS Homo sapiens
50 PSCA 8000
Affinity Capture-MS Homo sapiens
51 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
52 HSPA1A 3303
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
53 DNAJA1 3301
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
54 HSPA4 3308
Co-fractionation Homo sapiens
55 HPSE 10855
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 ERG  
Reconstituted Complex Homo sapiens
57 DNAJC3 5611
Affinity Capture-MS Homo sapiens
58 HYOU1 10525
Co-fractionation Homo sapiens
59 HSPA4L 22824
Co-fractionation Homo sapiens
60 HSPH1 10808
Co-fractionation Homo sapiens
61 PSMC6 5706
Co-fractionation Homo sapiens
62 TCEB1 6921
Affinity Capture-MS Homo sapiens
63 HSPA9 3313
Co-fractionation Homo sapiens
64 PSMC2 5701
Co-fractionation Homo sapiens
65 DNAJB1 3337
Affinity Capture-MS Homo sapiens
66 SRP14 6727
Proximity Label-MS Homo sapiens
67 ZNF146  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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