Gene description for CYB5R2
Gene name cytochrome b5 reductase 2
Gene symbol CYB5R2
Other names/aliases B5R.2
Species Homo sapiens
 Database cross references - CYB5R2
ExoCarta ExoCarta_51700
Vesiclepedia VP_51700
Entrez Gene 51700
HGNC 24376
MIM 608342
UniProt Q6BCY4  
 CYB5R2 identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for CYB5R2
Molecular Function
    cytochrome-b5 reductase activity, acting on NAD(P)H GO:0004128 IDA
    cytochrome-b5 reductase activity, acting on NAD(P)H GO:0004128 TAS
    protein binding GO:0005515 IPI
    FAD binding GO:0071949 IBA
Biological Process
    bicarbonate transport GO:0015701 TAS
    sterol biosynthetic process GO:0016126 IEA
Subcellular Localization
    nucleus GO:0005634 HDA
    mitochondrion GO:0005739 IBA
    endoplasmic reticulum membrane GO:0005789 TAS
    membrane GO:0016020 IDA
 Experiment description of studies that identified CYB5R2 in sEVs
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for CYB5R2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TRIP13 9319
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
2 TRAF2 7186
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
3 AGPAT1 10554
Affinity Capture-MS Homo sapiens
4 COIL  
Two-hybrid Homo sapiens
5 FFAR2  
Two-hybrid Homo sapiens
6 NFKBID  
Two-hybrid Homo sapiens
7 REL 5966
Two-hybrid Homo sapiens
8 FANCL 55120
Two-hybrid Homo sapiens
9 MED20 9477
Two-hybrid Homo sapiens
10 CDC23 8697
Two-hybrid Homo sapiens
11 TRIM55  
Two-hybrid Homo sapiens
12 GOLGA2 2801
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
13 ZNF778 197320
Two-hybrid Homo sapiens
14 SARAF  
Affinity Capture-MS Homo sapiens
15 PINK1  
Affinity Capture-MS Homo sapiens
16 MEOX2  
Two-hybrid Homo sapiens
17 AGR2 10551
Two-hybrid Homo sapiens
18 HGS 9146
Two-hybrid Homo sapiens
19 OR2A4 79541
Affinity Capture-MS Homo sapiens
20 PSMC6 5706
Two-hybrid Homo sapiens
21 CCR1  
Affinity Capture-MS Homo sapiens
22 TRIM63  
Two-hybrid Homo sapiens
23 TRAF1 7185
Two-hybrid Homo sapiens
24 SUOX  
Two-hybrid Homo sapiens
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