Gene description for ZNF778
Gene name zinc finger protein 778
Gene symbol ZNF778
Other names/aliases -
Species Homo sapiens
 Database cross references - ZNF778
ExoCarta ExoCarta_197320
Vesiclepedia VP_197320
Entrez Gene 197320
HGNC 26479
UniProt Q96MU6  
 ZNF778 identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for ZNF778
Molecular Function
    RNA polymerase II transcription regulatory region sequence-specific DNA binding GO:0000977 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    regulation of transcription by RNA polymerase II GO:0006357 IBA
Subcellular Localization
    nucleus GO:0005634 IBA
 Experiment description of studies that identified ZNF778 in sEVs
1
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for ZNF778
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KRTAP10-7  
Two-hybrid Homo sapiens
2 LTBP2 4053
Affinity Capture-MS Homo sapiens
3 SV2B  
Affinity Capture-MS Homo sapiens
4 SNX16  
Affinity Capture-MS Homo sapiens
5 TRIM28 10155
Affinity Capture-MS Homo sapiens
6 CYB5R2 51700
Two-hybrid Homo sapiens
7 VCP 7415
Affinity Capture-MS Homo sapiens
8 EFEMP1 2202
Affinity Capture-MS Homo sapiens
9 LRP4 4038
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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