Gene description for TRIM32
Gene name tripartite motif containing 32
Gene symbol TRIM32
Other names/aliases BBS11
HT2A
LGMD2H
TATIP
Species Homo sapiens
 Database cross references - TRIM32
ExoCarta ExoCarta_22954
Entrez Gene 22954
HGNC 16380
MIM 602290
UniProt Q13049  
 TRIM32 identified in exosomes derived from the following tissue/cell type
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for TRIM32
Molecular Function
    translation initiation factor binding GO:0031369 ISS
    ubiquitin protein ligase activity GO:0061630 IDA
    protein binding GO:0005515 IPI
    myosin binding GO:0017022 ISS
    RNA binding GO:0003723 ISS
    Tat protein binding GO:0030957 TAS
    transcription coactivator activity GO:0003713 TAS
    zinc ion binding GO:0008270 IEA
    protein self-association GO:0043621 IDA
    ubiquitin-protein transferase activity GO:0004842 IDA
    ubiquitin binding GO:0043130 IDA
    ligase activity GO:0016874 IEA
Biological Process
    positive regulation of type I interferon production GO:0032481 TAS
    protein ubiquitination involved in ubiquitin-dependent protein catabolic process GO:0042787 IMP
    positive regulation of sequence-specific DNA binding transcription factor activity GO:0051091 IDA
    protein ubiquitination GO:0016567 IDA
    positive regulation of neurogenesis GO:0050769 ISS
    response to UV GO:0009411 ISS
    positive regulation of cell migration GO:0030335 IDA
    positive regulation of cell cycle GO:0045787 IDA
    positive regulation of proteolysis GO:0045862 IDA
    negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage GO:1902230 IDA
    negative regulation of viral transcription GO:0032897 IDA
    fat cell differentiation GO:0045444 ISS
    negative regulation of viral release from host cell GO:1902187 IDA
    positive regulation of NF-kappaB transcription factor activity GO:0051092 ISS
    positive regulation of cell motility GO:2000147 ISS
    negative regulation of fibroblast proliferation GO:0048147 ISS
    innate immune response GO:0045087 TAS
    protein polyubiquitination GO:0000209 IDA
    positive regulation of protein catabolic process GO:0045732 ISS
    positive regulation of neuron differentiation GO:0045666 ISS
    regulation of type I interferon production GO:0032479 TAS
    positive regulation of cell growth GO:0030307 IDA
    response to tumor necrosis factor GO:0034612 ISS
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 IDA
Subcellular Localization
    cytosol GO:0005829 TAS
    nucleus GO:0005634 ISS
    striated muscle myosin thick filament GO:0005863 ISS
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified TRIM32 in exosomes
1
Experiment ID 255
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 256
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 257
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 193
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63|CD9
EV Membrane markers
PHB
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 194
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 195
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TRIM32
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TRIM32 22954
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
2 SFN 2810
Affinity Capture-MS Homo sapiens
3 RFP  
Two-hybrid Homo sapiens
4 UBQLN1 29979
Two-hybrid Homo sapiens
5 TRIM23 373
Two-hybrid Homo sapiens
6 MID2 11043
Two-hybrid Homo sapiens
7 PDE9A  
Two-hybrid Homo sapiens
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