Gene description for NDRG2
Gene name NDRG family member 2
Gene symbol NDRG2
Other names/aliases SYLD
Species Homo sapiens
 Database cross references - NDRG2
ExoCarta ExoCarta_57447
Entrez Gene 57447
HGNC 14460
MIM 605272
UniProt Q9UN36  
 NDRG2 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Saliva 19199708    
 Gene ontology annotations for NDRG2
Molecular Function
    protein binding GO:0005515 IPI
    molecular_function GO:0003674 ND
Biological Process
    regulation of platelet-derived growth factor production GO:0090361 IEA
    regulation of vascular endothelial growth factor production GO:0010574 IEA
    negative regulation of cytokine production GO:0001818 IEA
    negative regulation of smooth muscle cell proliferation GO:0048662 IEA
    Wnt signaling pathway GO:0016055 IEA
    substantia nigra development GO:0021762 IEP
    signal transduction GO:0007165 IBA
    cell differentiation GO:0030154 IEA
    negative regulation of ERK1 and ERK2 cascade GO:0070373 IEA
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    centrosome GO:0005813 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IDA
    growth cone GO:0030426 IEA
    perinuclear region of cytoplasm GO:0048471 IEA
    cytosol GO:0005829 NAS
    Golgi apparatus GO:0005794 IDA
    microtubule organizing center GO:0005815 IDA
 Experiment description of studies that identified NDRG2 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 66
ISEV standards
IEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD63|CD81
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for NDRG2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which NDRG2 is involved
No pathways found





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