Gene description for VPS11
Gene name vacuolar protein sorting 11 homolog (S. cerevisiae)
Gene symbol VPS11
Other names/aliases END1
PEP5
RNF108
hVPS11
Species Homo sapiens
 Database cross references - VPS11
ExoCarta ExoCarta_55823
Entrez Gene 55823
HGNC 14583
MIM 608549
UniProt Q9H270  
 VPS11 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Thymus 23844026    
 Gene ontology annotations for VPS11
Molecular Function
    nucleotide binding GO:0000166 IEA
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IEA
    syntaxin binding GO:0019905 IDA
    protein domain specific binding GO:0019904 IPI
Biological Process
    endosomal vesicle fusion GO:0034058 IMP
    positive regulation of early endosome to late endosome transport GO:2000643 IMP
    regulation of organelle assembly GO:1902115 IMP
    positive regulation of protein targeting to mitochondrion GO:1903955 IMP
    intracellular protein transport GO:0006886 IEA
    regulation of protein stability GO:0031647 IMP
    positive regulation of cellular protein catabolic process GO:1903364 IMP
Subcellular Localization
    lysosome GO:0005764 IDA
    endocytic vesicle GO:0030139 IDA
    early endosome GO:0005769 IDA
    late endosome membrane GO:0031902 IEA
    HOPS complex GO:0030897 IDA
    lysosomal membrane GO:0005765 IDA
    late endosome GO:0005770 IDA
    endosome GO:0005768 IDA
 Experiment description of studies that identified VPS11 in exosomes
1
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for VPS11
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 VPS33A 65082
Invivo Homo sapiens
Invivo Homo sapiens
2 STX7 8417
Invivo Homo sapiens
3 BCAR1 9564
Two-hybrid Homo sapiens
4 VPS16 64601
Invivo Homo sapiens
Invivo Homo sapiens
5 VPS18 57617
Invivo Homo sapiens
Invivo Homo sapiens
6 LSM1  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
7 MARK3 4140
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which VPS11 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here