Gene description for VPS8
Gene name vacuolar protein sorting 8 homolog (S. cerevisiae)
Gene symbol VPS8
Other names/aliases KIAA0804
Species Homo sapiens
 Database cross references - VPS8
ExoCarta ExoCarta_23355
Vesiclepedia VP_23355
Entrez Gene 23355
HGNC 29122
UniProt Q8N3P4  
 VPS8 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for VPS8
Molecular Function
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    protein targeting to vacuole GO:0006623 IBA
    endosomal vesicle fusion GO:0034058 IBA
    endosomal vesicle fusion GO:0034058 IMP
    endosomal vesicle fusion GO:0034058 NAS
    regulation of SNARE complex assembly GO:0035542 NAS
Subcellular Localization
    early endosome GO:0005769 IBA
    early endosome GO:0005769 IDA
    late endosome GO:0005770 IBA
    HOPS complex GO:0030897 IBA
    CORVET complex GO:0033263 IBA
    CORVET complex GO:0033263 NAS
 Experiment description of studies that identified VPS8 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for VPS8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GYPA  
Affinity Capture-MS Homo sapiens
2 ANKFY1 51479
Proximity Label-MS Homo sapiens
3 VPS16 64601
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
4 C5AR1  
Affinity Capture-MS Homo sapiens
5 ALDH3A2 224
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 CYB5B 80777
Affinity Capture-MS Homo sapiens
7 KIF14 9928
Affinity Capture-MS Homo sapiens
8 OCLN 100506658
Proximity Label-MS Homo sapiens
9 GPR17 2840
Affinity Capture-MS Homo sapiens
10 SIGLECL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 LAMTOR1 55004
Proximity Label-MS Homo sapiens
12 BTNL9  
Affinity Capture-MS Homo sapiens
13 RAB5A 5868
Proximity Label-MS Homo sapiens
14 TGFBRAP1 9392
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
15 HCST  
Affinity Capture-MS Homo sapiens
16 FZD10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 EFNB1 1947
Affinity Capture-MS Homo sapiens
18 RAB9A 9367
Proximity Label-MS Homo sapiens
19 HIST2H2AA3 8337
Affinity Capture-MS Homo sapiens
20 SUB1 10923
Affinity Capture-MS Homo sapiens
21 FPR1  
Affinity Capture-MS Homo sapiens
22 GCGR  
Affinity Capture-MS Homo sapiens
23 C19orf38  
Affinity Capture-MS Homo sapiens
24 LAMP2 3920
Proximity Label-MS Homo sapiens
25 EDNRB 1910
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 HIST1H2BD 3017
Affinity Capture-MS Homo sapiens
27 TTK 7272
Affinity Capture-MS Homo sapiens
28 FAXC  
Affinity Capture-MS Homo sapiens
29 PTGER3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 PNKD  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 VPS33A 65082
Affinity Capture-MS Homo sapiens
32 RAB4A 5867
Proximity Label-MS Homo sapiens
33 APEX1 328
Affinity Capture-RNA Homo sapiens
34 Kif5b 16573
Affinity Capture-MS Mus musculus
35 COMTD1 118881
Affinity Capture-MS Homo sapiens
36 UXS1 80146
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 VIPR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 C3orf18  
Affinity Capture-MS Homo sapiens
39 SMAD9  
Two-hybrid Homo sapiens
40 NTRK1 4914
Affinity Capture-MS Homo sapiens
41 GPR45  
Affinity Capture-MS Homo sapiens
42 RAB7A 7879
Proximity Label-MS Homo sapiens
43 RAB11A 8766
Proximity Label-MS Homo sapiens
44 KIR2DL4  
Affinity Capture-MS Homo sapiens
45 Kif22  
Affinity Capture-MS Mus musculus
46 ATG9A 79065
Proximity Label-MS Homo sapiens
47 ITPRIP  
Affinity Capture-MS Homo sapiens
48 RAB5C 5878
Proximity Label-MS Homo sapiens
49 PRMT1 3276
Affinity Capture-MS Homo sapiens
50 MLNR  
Affinity Capture-MS Homo sapiens
51 VPS18 57617
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 KIF2B 84643
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 C16orf58 64755
Affinity Capture-MS Homo sapiens
54 VPS11 55823
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
55 AVPR2  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which VPS8 is involved
No pathways found





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