Gene description for VPS16
Gene name vacuolar protein sorting 16 homolog (S. cerevisiae)
Gene symbol VPS16
Other names/aliases hVPS16
Species Homo sapiens
 Database cross references - VPS16
ExoCarta ExoCarta_64601
Vesiclepedia VP_64601
Entrez Gene 64601
HGNC 14584
MIM 608550
UniProt Q9H269  
 VPS16 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Thymus 23844026    
 Gene ontology annotations for VPS16
Molecular Function
    actin binding GO:0003779 IBA
    protein binding GO:0005515 IPI
    actin filament binding GO:0051015 IEA
Biological Process
    intracellular protein transport GO:0006886 IEA
    endosome to lysosome transport GO:0008333 IMP
    endosomal transport GO:0016197 IBA
    endosomal vesicle fusion GO:0034058 NAS
    regulation of SNARE complex assembly GO:0035542 NAS
    vacuole fusion, non-autophagic GO:0042144 IBA
    autophagosome maturation GO:0097352 IMP
Subcellular Localization
    lysosome GO:0005764 IDA
    lysosomal membrane GO:0005765 HDA
    endosome GO:0005768 IBA
    early endosome GO:0005769 IDA
    late endosome GO:0005770 IDA
    autophagosome GO:0005776 IEA
    endosome membrane GO:0010008 TAS
    clathrin-coated vesicle GO:0030136 IEA
    axon GO:0030424 IEA
    HOPS complex GO:0030897 IBA
    HOPS complex GO:0030897 IDA
    HOPS complex GO:0030897 NAS
    late endosome membrane GO:0031902 IEA
    CORVET complex GO:0033263 NAS
    neuronal cell body GO:0043025 IEA
    recycling endosome GO:0055037 IDA
    presynapse GO:0098793 IEA
 Experiment description of studies that identified VPS16 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for VPS16
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 VPS41 27072
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
2 HOOK1  
Affinity Capture-Western Homo sapiens
3 FAM167A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 TBC1D4  
Affinity Capture-MS Homo sapiens
5 IPO7 10527
Affinity Capture-MS Homo sapiens
6 TNPO1 3842
Co-fractionation Homo sapiens
7 RPA2 6118
Proximity Label-MS Homo sapiens
8 C9orf78 51759
Affinity Capture-MS Homo sapiens
9 CT55  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 HOOK2  
Affinity Capture-Western Homo sapiens
11 PRPF40A 55660
Affinity Capture-MS Homo sapiens
12 D2HGDH 728294
Affinity Capture-MS Homo sapiens
13 HARS2 23438
Affinity Capture-MS Homo sapiens
14 FBXW7  
Synthetic Lethality Homo sapiens
Affinity Capture-MS Homo sapiens
15 CIB3  
Affinity Capture-MS Homo sapiens
16 PSMB6 5694
Co-fractionation Homo sapiens
17 SEMA4C 54910
Affinity Capture-MS Homo sapiens
18 KIAA1191  
Affinity Capture-MS Homo sapiens
19 EPHA1 2041
Affinity Capture-MS Homo sapiens
20 BRD4 23476
Affinity Capture-MS Homo sapiens
21 PKNOX1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 AKTIP  
Affinity Capture-Western Homo sapiens
23 NPAS1  
Affinity Capture-MS Homo sapiens
24 RPS11 6205
Affinity Capture-MS Homo sapiens
25 FBXW5  
Affinity Capture-MS Homo sapiens
26 C9orf163  
Affinity Capture-MS Homo sapiens
27 Plk2  
Affinity Capture-Western Rattus norvegicus
Reconstituted Complex Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
28 FAM96A  
Affinity Capture-MS Homo sapiens
29 SULT1C4  
Affinity Capture-MS Homo sapiens
30 MRPL54  
Affinity Capture-MS Homo sapiens
31 PARK2  
Affinity Capture-MS Homo sapiens
32 B4GALT2  
Affinity Capture-MS Homo sapiens
33 STX17 55014
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
34 ACVR1B 91
Affinity Capture-MS Homo sapiens
35 SREBF2 6721
Negative Genetic Homo sapiens
36 MTMR7  
Affinity Capture-MS Homo sapiens
37 LRRC29  
Affinity Capture-MS Homo sapiens
38 ATP9A 10079
Affinity Capture-MS Homo sapiens
39 NTRK1 4914
Affinity Capture-MS Homo sapiens
40 RILP 83547
Reconstituted Complex Homo sapiens
41 VCPIP1 80124
Affinity Capture-MS Homo sapiens
42 LYPD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 VPS18 57617
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
44 TTC31  
Affinity Capture-MS Homo sapiens
45 HSPB8 26353
Affinity Capture-MS Homo sapiens
46 UBXN6 80700
Affinity Capture-MS Homo sapiens
47 TECPR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 WHAMMP3  
Affinity Capture-MS Homo sapiens
49 CXCR4 7852
Affinity Capture-MS Homo sapiens
50 MON1B 22879
Reconstituted Complex Homo sapiens
51 EGFR 1956
Negative Genetic Homo sapiens
52 HOOK3 84376
Affinity Capture-Western Homo sapiens
53 PTK2 5747
Affinity Capture-MS Homo sapiens
54 DUSP16  
Affinity Capture-MS Homo sapiens
55 WAPAL  
Co-fractionation Homo sapiens
56 GNG13  
Two-hybrid Homo sapiens
57 UVRAG  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
58 CCDC127  
Affinity Capture-MS Homo sapiens
59 PIAS1 8554
Affinity Capture-MS Homo sapiens
60 RPA3 6119
Proximity Label-MS Homo sapiens
61 SOHLH1  
Affinity Capture-MS Homo sapiens
62 AMZ1  
Affinity Capture-MS Homo sapiens
63 MTOR 2475
Affinity Capture-MS Homo sapiens
64 RAB9A 9367
Proximity Label-MS Homo sapiens
65 SRSF2 6427
Affinity Capture-MS Homo sapiens
66 ESRP2 80004
Affinity Capture-MS Homo sapiens
67 MRPS17 51373
Affinity Capture-MS Homo sapiens
68 TSR1 55720
Co-fractionation Homo sapiens
69 ZNF417  
Two-hybrid Homo sapiens
70 ZRANB1 54764
Affinity Capture-MS Homo sapiens
71 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
72 FASN 2194
Negative Genetic Homo sapiens
73 TP53 7157
Affinity Capture-MS Homo sapiens
74 EXOC8 149371
Two-hybrid Homo sapiens
75 TGFBRAP1 9392
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 PIN1 5300
Affinity Capture-MS Homo sapiens
77 C1QTNF6 114904
Affinity Capture-MS Homo sapiens
78 MRPS15  
Affinity Capture-MS Homo sapiens
79 VPS8 23355
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 VPS33A 65082
Co-fractionation Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
81 RIOK2 55781
Affinity Capture-MS Homo sapiens
82 STXBP4 252983
Affinity Capture-MS Homo sapiens
83 INF2 64423
Affinity Capture-MS Homo sapiens
84 RAB7A 7879
Reconstituted Complex Homo sapiens
Proximity Label-MS Homo sapiens
85 ZNF800  
Affinity Capture-MS Homo sapiens
86 ZUFSP 221302
Affinity Capture-MS Homo sapiens
87 KRAS 3845
Negative Genetic Homo sapiens
88 VPS11 55823
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
89 MOCS3 27304
Affinity Capture-MS Homo sapiens
90 Vps41  
Affinity Capture-MS Mus musculus
91 CFH 3075
Affinity Capture-MS Homo sapiens
92 PMM1 5372
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here