Gene description for VPS16
Gene name vacuolar protein sorting 16 homolog (S. cerevisiae)
Gene symbol VPS16
Other names/aliases hVPS16
Species Homo sapiens
 Database cross references - VPS16
ExoCarta ExoCarta_64601
Entrez Gene 64601
HGNC 14584
MIM 608550
UniProt Q9H269  
 VPS16 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Thymus 23844026    
 Gene ontology annotations for VPS16
Molecular Function
    actin filament binding GO:0051015 IEA
    actin binding GO:0003779 IBA
Biological Process
    regulation of vacuole fusion, non-autophagic GO:0032889 IBA
    vacuole organization GO:0007033 IEA
    regulation of SNARE complex assembly GO:0035542 IBA
    intracellular protein transport GO:0006886 IEA
    endosomal transport GO:0016197 IBA
    viral entry into host cell GO:0046718 IEA
Subcellular Localization
    axon GO:0030424 IEA
    HOPS complex GO:0030897 IDA
    late endosome membrane GO:0031902 IEA
    early endosome GO:0005769 IDA
    late endosome GO:0005770 IDA
    neuronal cell body GO:0043025 IEA
    actin filament GO:0005884 IEA
    recycling endosome GO:0055037 IDA
    lysosomal membrane GO:0005765 IDA
    lysosome GO:0005764 IDA
 Experiment description of studies that identified VPS16 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for VPS16
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 VPS16 64601
Invivo Homo sapiens
Invivo Homo sapiens
2 VPS33A 65082
Invivo Homo sapiens
3 VPS11 55823
Invivo Homo sapiens
Invivo Homo sapiens
4 STX7 8417
Invivo Homo sapiens
5 VPS18 57617
Invivo Homo sapiens
Invivo Homo sapiens
View the network image/svg+xml
 Pathways in which VPS16 is involved
No pathways found





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