Gene description for CFH
Gene name complement factor H
Gene symbol CFH
Other names/aliases AHUS1
AMBP1
ARMD4
ARMS1
CFHL3
FH
FHL1
HF
HF1
HF2
HUS
Species Homo sapiens
 Database cross references - CFH
ExoCarta ExoCarta_3075
Entrez Gene 3075
HGNC 4883
MIM 134370
UniProt P08603  
 CFH identified in exosomes derived from the following tissue/cell type
Malignant pleural effusions 14975938    
Malignant pleural effusions 14975938    
Urine 19056867    
 Gene ontology annotations for CFH
Molecular Function
    heparan sulfate proteoglycan binding GO:0043395 IDA
    heparin binding GO:0008201 IDA
    protein binding GO:0005515 IPI
Biological Process
    complement activation GO:0006956 TAS
    innate immune response GO:0045087 TAS
    complement activation, alternative pathway GO:0006957 IEA
    regulation of complement activation GO:0030449 TAS
Subcellular Localization
    blood microparticle GO:0072562 IDA
    extracellular space GO:0005615 TAS
    extracellular exosome GO:0070062 IDA
    extracellular region GO:0005576 TAS
 Experiment description of studies that identified CFH in exosomes
1
Experiment ID 51
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP90
EV Cytosolic markers
✔
MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 14975938    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes isolated from human malignant pleural effusions.
Authors Bard MP, Hegmans JP, Hemmes A, Luider TM, Willemsen R, Severijnen LA, van Meerbeeck JP, Burgers SA, Hoogsteden HC, Lambrecht BN
Journal name AJRCMB
Publication year 2004
Sample Malignant pleural effusions
Sample name Pleural Fluid - Breast cancer
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
2
Experiment ID 53
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP90
EV Cytosolic markers
✔
MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 14975938    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes isolated from human malignant pleural effusions.
Authors Bard MP, Hegmans JP, Hemmes A, Luider TM, Willemsen R, Severijnen LA, van Meerbeeck JP, Burgers SA, Hoogsteden HC, Lambrecht BN
Journal name AJRCMB
Publication year 2004
Sample Malignant pleural effusions
Sample name Pleural Fluid - Mesothelioma
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
3
Experiment ID 63
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for CFH
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CFI 3426
Invitro Homo sapiens
2 CFHL1  
Affinity Capture-MS Homo sapiens
3 ADM 133
Invitro Homo sapiens
4 DMP1  
Invitro Homo sapiens
5 HEM12  
Far Western 4932
6 SELL 6402
Reconstituted Complex Homo sapiens
7 ITGAM 3684
Invitro Homo sapiens
8 NCL1  
Far Western 4932
9 HIP1  
Far Western 4932
10 C3 718
Invitro Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
Reconstituted Complex Homo sapiens
11 GPM1  
Far Western 4932
12 CRP  
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which CFH is involved
PathwayEvidenceSource
Regulation of Complement cascade TAS Reactome





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