Gene description for ATP9A
Gene name ATPase, class II, type 9A
Gene symbol ATP9A
Other names/aliases ATPIIA
Species Homo sapiens
 Database cross references - ATP9A
ExoCarta ExoCarta_10079
Vesiclepedia VP_10079
Entrez Gene 10079
HGNC 13540
MIM 609126
UniProt O75110  
 ATP9A identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for ATP9A
Molecular Function
    magnesium ion binding GO:0000287 IEA
    protease binding GO:0002020 IPI
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
    ATPase-coupled intramembrane lipid transporter activity GO:0140326 IBA
Biological Process
    retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum GO:0006890 IBA
    endocytosis GO:0006897 IBA
    phospholipid translocation GO:0045332 IBA
    phospholipid translocation GO:0045332 NAS
    neuron projection morphogenesis GO:0048812 ISS
    negative regulation of exosomal secretion GO:1903542 IMP
    regulation of retrograde transport, endosome to Golgi GO:1905279 IMP
    regulation of endocytic recycling GO:2001135 IMP
Subcellular Localization
    endosome GO:0005768 IBA
    early endosome GO:0005769 IDA
    late endosome GO:0005770 IDA
    trans-Golgi network GO:0005802 IBA
    trans-Golgi network GO:0005802 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    early endosome membrane GO:0031901 IDA
    late endosome membrane GO:0031902 IEA
    trans-Golgi network membrane GO:0032588 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    recycling endosome GO:0055037 IDA
    recycling endosome membrane GO:0055038 IDA
 Experiment description of studies that identified ATP9A in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ATP9A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZNF644  
Affinity Capture-MS Homo sapiens
2 NES 10763
Affinity Capture-MS Homo sapiens
3 CLPP 8192
Proximity Label-MS Homo sapiens
4 STARD3NL 83930
Affinity Capture-MS Homo sapiens
5 VPS16 64601
Affinity Capture-MS Homo sapiens
6 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
7 SLC15A1  
Affinity Capture-MS Homo sapiens
8 ATP13A3 79572
Affinity Capture-MS Homo sapiens
9 LAMTOR1 55004
Proximity Label-MS Homo sapiens
10 RIC3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 Myh9 17886
Affinity Capture-MS Mus musculus
12 LMTK2 22853
Affinity Capture-MS Homo sapiens
13 Psmb5 19173
Affinity Capture-MS Mus musculus
14 RAB7A 7879
Proximity Label-MS Homo sapiens
15 Psmb4 19172
Affinity Capture-MS Mus musculus
16 RAB9A 9367
Proximity Label-MS Homo sapiens
17 SLC39A4 55630
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 GPR35  
Affinity Capture-MS Homo sapiens
19 COMMD8 54951
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 LRRC55  
Affinity Capture-MS Homo sapiens
21 CHRM5  
Two-hybrid Homo sapiens
22 KLHL42  
Affinity Capture-MS Homo sapiens
23 C3AR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 ACKR2  
Affinity Capture-MS Homo sapiens
25 CRELD1 78987
Affinity Capture-MS Homo sapiens
26 IFT57 55081
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 SLC1A5 6510
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 TSPAN15 23555
Affinity Capture-MS Homo sapiens
29 CHCHD2  
Affinity Capture-MS Homo sapiens
30 ATP2B4 493
Co-fractionation Homo sapiens
31 B4GAT1 11041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 VAMP3 9341
Affinity Capture-MS Homo sapiens
33 COX6B1 1340
Affinity Capture-MS Homo sapiens
34 ATP9B 374868
Affinity Capture-MS Homo sapiens
35 TMEM74  
Affinity Capture-MS Homo sapiens
36 LPAR1 1902
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 P2RY2 5029
Affinity Capture-MS Homo sapiens
38 ESR1  
Affinity Capture-MS Homo sapiens
39 RAB4A 5867
Proximity Label-MS Homo sapiens
40 SYP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 RAB3B 5865
Proximity Label-MS Homo sapiens
42 RELL2  
Affinity Capture-MS Homo sapiens
43 FAM134C 162427
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 PBXIP1 57326
Affinity Capture-MS Homo sapiens
45 ATP2A1 487
Affinity Capture-MS Homo sapiens
46 SPPL2B 56928
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 SH3GLB1 51100
Affinity Capture-MS Homo sapiens
48 TMED7 51014
Affinity Capture-MS Homo sapiens
49 ATP2B2 491
Affinity Capture-MS Homo sapiens
50 COPS6 10980
Affinity Capture-MS Homo sapiens
51 ACADM 34
Co-fractionation Homo sapiens
52 LPAR2 9170
Affinity Capture-MS Homo sapiens
53 ABCC3 8714
Co-fractionation Homo sapiens
54 PLK4  
Affinity Capture-MS Homo sapiens
55 IPPK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 TMED2 10959
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 STX7 8417
Proximity Label-MS Homo sapiens
58 KCNA2  
Affinity Capture-MS Homo sapiens
59 NIPAL1  
Affinity Capture-MS Homo sapiens
60 YIPF3 25844
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 CCR6  
Affinity Capture-MS Homo sapiens
62 CTR9 9646
Affinity Capture-MS Homo sapiens
63 CMKLR1 1240
Affinity Capture-MS Homo sapiens
64 RANBP6 26953
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 SCN3B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 TSPAN10 83882
Affinity Capture-MS Homo sapiens
67 FPR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 SLC22A9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 DGCR2 9993
Affinity Capture-MS Homo sapiens
70 RAB11A 8766
Proximity Label-MS Homo sapiens
71 STX12 23673
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ATP9A is involved
PathwayEvidenceSource
Ion channel transport TAS Reactome
Ion transport by P-type ATPases TAS Reactome
Transport of small molecules TAS Reactome





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