Gene description for ABCC3
Gene name ATP-binding cassette, sub-family C (CFTR/MRP), member 3
Gene symbol ABCC3
Other names/aliases ABC31
EST90757
MLP2
MOAT-D
MRP3
cMOAT2
Species Homo sapiens
 Database cross references - ABCC3
ExoCarta ExoCarta_8714
Vesiclepedia VP_8714
Entrez Gene 8714
HGNC 54
MIM 604323
UniProt O15438  
 ABCC3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
 Gene ontology annotations for ABCC3
Molecular Function
    ATP binding GO:0005524 IEA
    ABC-type xenobiotic transporter activity GO:0008559 IDA
    ABC-type xenobiotic transporter activity GO:0008559 IMP
    glucuronoside transmembrane transporter activity GO:0015164 IDA
    ABC-type glutathione S-conjugate transporter activity GO:0015431 IDA
    ABC-type bile acid transporter activity GO:0015432 ISS
    ABC-type bile acid transporter activity GO:0015432 TAS
    ATP hydrolysis activity GO:0016887 IEA
    ATPase-coupled transmembrane transporter activity GO:0042626 IBA
    ATPase-coupled transmembrane transporter activity GO:0042626 IDA
    ATPase-coupled transmembrane transporter activity GO:0042626 TAS
    xenobiotic transmembrane transporter activity GO:0042910 IDA
    ATPase-coupled inorganic anion transmembrane transporter activity GO:0043225 TAS
    icosanoid transmembrane transporter activity GO:0071714 IDA
    ABC-type transporter activity GO:0140359 TAS
Biological Process
    xenobiotic metabolic process GO:0006805 TAS
    xenobiotic transmembrane transport GO:0006855 IMP
    bile acid and bile salt transport GO:0015721 ISS
    bile acid and bile salt transport GO:0015721 TAS
    glucuronoside transport GO:0015779 IEA
    xenobiotic transport GO:0042908 IDA
    transmembrane transport GO:0055085 IBA
    transmembrane transport GO:0055085 TAS
    leukotriene transport GO:0071716 IMP
    monoatomic anion transmembrane transport GO:0098656 IEA
    transport across blood-brain barrier GO:0150104 NAS
Subcellular Localization
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    basal plasma membrane GO:0009925 IDA
    membrane GO:0016020 IBA
    basolateral plasma membrane GO:0016323 IDA
 Experiment description of studies that identified ABCC3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
 Protein-protein interactions for ABCC3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 IDH3A 3419
Co-fractionation Homo sapiens
2 ATP9A 10079
Co-fractionation Homo sapiens
3 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
4 VDAC1 7416
Co-fractionation Homo sapiens
5 CRELD1 78987
Affinity Capture-MS Homo sapiens
6 VDAC3 7419
Co-fractionation Homo sapiens
7 NR3C1 2908
Proximity Label-MS Homo sapiens
8 SREBF2 6721
Negative Genetic Homo sapiens
9 MLH1 4292
Two-hybrid Homo sapiens
10 CANX 821
Co-fractionation Homo sapiens
11 EEF2 1938
Co-fractionation Homo sapiens
12 VDAC2 7417
Co-fractionation Homo sapiens
13 VCP 7415
Co-fractionation Homo sapiens
View the network image/svg+xml



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