Gene description for MOCS3
Gene name molybdenum cofactor synthesis 3
Gene symbol MOCS3
Other names/aliases UBA4
Species Homo sapiens
 Database cross references - MOCS3
ExoCarta ExoCarta_27304
Vesiclepedia VP_27304
Entrez Gene 27304
HGNC 15765
MIM 609277
UniProt O95396  
 MOCS3 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
Thymus 23844026    
 Gene ontology annotations for MOCS3
Molecular Function
    protein adenylyltransferase activity GO:0070733 IBA
    URM1 activating enzyme activity GO:0042292 IDA
    metal ion binding GO:0046872 IEA
    ATP binding GO:0005524 IEA
    molybdopterin-synthase adenylyltransferase activity GO:0061605 IEA
    sulfurtransferase activity GO:0016783 TAS
    nucleotidyltransferase activity GO:0016779 IDA
    protein binding GO:0005515 IPI
    thiosulfate sulfurtransferase activity GO:0004792 IMP
    molybdopterin-synthase sulfurtransferase activity GO:0061604 IEA
Biological Process
    enzyme active site formation via L-cysteine persulfide GO:0018192 IDA
    tRNA wobble uridine modification GO:0002098 IDA
    small molecule metabolic process GO:0044281 TAS
    water-soluble vitamin metabolic process GO:0006767 TAS
    protein urmylation GO:0032447 IBA
    molybdopterin cofactor biosynthetic process GO:0032324 TAS
    vitamin metabolic process GO:0006766 TAS
    protein adenylylation GO:0018117 IBA
    Mo-molybdopterin cofactor biosynthetic process GO:0006777 IDA
    tRNA thio-modification GO:0034227 IDA
    tRNA wobble position uridine thiolation GO:0002143 IBA
Subcellular Localization
    cytosol GO:0005829 TAS
 Experiment description of studies that identified MOCS3 in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 275
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
EV Enriched markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
3
Experiment ID 274
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
EV Enriched markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
4
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MOCS3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 XRN2 22803
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
2 EXOSC6 118460
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which MOCS3 is involved
PathwayEvidenceSource
Molybdenum cofactor biosynthesis TAS Reactome





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