Gene description for DYNC2H1
Gene name dynein, cytoplasmic 2, heavy chain 1
Gene symbol DYNC2H1
Other names/aliases ATD3
DHC1b
DHC2
DNCH2
DYH1B
SRPS2B
SRTD3
hdhc11
Species Homo sapiens
 Database cross references - DYNC2H1
ExoCarta ExoCarta_79659
Vesiclepedia VP_79659
Entrez Gene 79659
HGNC 2962
MIM 603297
UniProt Q8NCM8  
 DYNC2H1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Embryonic kidney cells 36706192    
Embryonic kidney cells 36706192    
Mesenchymal stem cells 36408942    
Urine 19056867    
 Gene ontology annotations for DYNC2H1
Molecular Function
    cytoskeletal motor activity GO:0003774 NAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    minus-end-directed microtubule motor activity GO:0008569 IDA
    ATP hydrolysis activity GO:0016887 IEA
    dynein intermediate chain binding GO:0045505 IBA
    dynein light intermediate chain binding GO:0051959 IBA
Biological Process
    kidney development GO:0001822 IEA
    Golgi organization GO:0007030 IDA
    determination of left/right symmetry GO:0007368 IEA
    dorsal/ventral pattern formation GO:0009953 IEA
    protein processing GO:0016485 IEA
    spinal cord motor neuron differentiation GO:0021522 IEA
    embryonic limb morphogenesis GO:0030326 IEA
    forebrain development GO:0030900 IEA
    intraciliary retrograde transport GO:0035721 IBA
    positive regulation of smoothened signaling pathway GO:0045880 IEA
    cilium assembly GO:0060271 IBA
    cilium movement involved in cell motility GO:0060294 IBA
    coronary vasculature development GO:0060976 IEA
    protein localization to cilium GO:0061512 IEA
    non-motile cilium assembly GO:1905515 IEA
Subcellular Localization
    Golgi apparatus GO:0005794 IDA
    cytoplasmic dynein complex GO:0005868 IBA
    cytoplasmic dynein complex GO:0005868 IDA
    microtubule GO:0005874 IDA
    plasma membrane GO:0005886 IEA
    cilium GO:0005929 TAS
    axoneme GO:0005930 IBA
    apical part of cell GO:0045177 IEA
    extracellular exosome GO:0070062 HDA
    ciliary tip GO:0097542 TAS
    9+2 motile cilium GO:0097729 IBA
 Experiment description of studies that identified DYNC2H1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 1275
MISEV standards
Biophysical techniques
✔
CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 1280
MISEV standards
Biophysical techniques
✔
TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T - Fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density 1.151 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
12
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for DYNC2H1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DNAJC7 7266
Proximity Label-MS Homo sapiens
2 DYNLL2 140735
Affinity Capture-MS Homo sapiens
3 ANLN 54443
Affinity Capture-MS Homo sapiens
4 GYPA  
Affinity Capture-MS Homo sapiens
5 ARMC5 79798
Affinity Capture-MS Homo sapiens
6 Evpl  
Affinity Capture-MS Mus musculus
7 IL17RA 23765
Affinity Capture-MS Homo sapiens
8 GAN 8139
Affinity Capture-MS Homo sapiens
9 Pxdc1  
Affinity Capture-MS Mus musculus
10 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
11 Tubg1 103733
Affinity Capture-MS Mus musculus
12 USP50  
Affinity Capture-MS Homo sapiens
13 LGI1  
Affinity Capture-MS Homo sapiens
14 HIST1H3A 8350
Cross-Linking-MS (XL-MS) Homo sapiens
15 DYNC2LI1 51626
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
16 KIF14 9928
Affinity Capture-MS Homo sapiens
17 IFT20 90410
Affinity Capture-MS Homo sapiens
18 NR3C1 2908
Proximity Label-MS Homo sapiens
19 RUVBL2 10856
Co-fractionation Homo sapiens
20 Wdr5  
Affinity Capture-MS Mus musculus
21 NPTN 27020
Affinity Capture-MS Homo sapiens
22 CRYL1 51084
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 VCPIP1 80124
Affinity Capture-MS Homo sapiens
24 SPC24 147841
Affinity Capture-MS Homo sapiens
25 KIF20A 10112
Affinity Capture-MS Homo sapiens
26 TIGD6  
Affinity Capture-MS Homo sapiens
27 C19orf38  
Affinity Capture-MS Homo sapiens
28 Vangl1  
Affinity Capture-MS Mus musculus
29 PPIA 5478
Cross-Linking-MS (XL-MS) Homo sapiens
30 MTNR1B  
Two-hybrid Homo sapiens
31 SEMA4C 54910
Affinity Capture-MS Homo sapiens
32 FCGRT 2217
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 DUSP16  
Affinity Capture-MS Homo sapiens
34 NONO 4841
Co-fractionation Homo sapiens
35 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
36 SF3A1 10291
Cross-Linking-MS (XL-MS) Homo sapiens
37 SNAPC4  
Affinity Capture-MS Homo sapiens
38 MKRN2 23609
Affinity Capture-RNA Homo sapiens
39 SPAG7  
Co-fractionation Homo sapiens
40 ARHGAP25  
Affinity Capture-MS Homo sapiens
41 PPP2R3C  
Affinity Capture-MS Homo sapiens
42 Cobll1 319876
Affinity Capture-MS Mus musculus
43 SS18L2  
Proximity Label-MS Homo sapiens
44 NAA11 84779
Affinity Capture-MS Homo sapiens
45 PRKY  
Affinity Capture-MS Homo sapiens
46 PEX14 5195
Co-purification Homo sapiens
47 GDI1 2664
Affinity Capture-MS Homo sapiens
48 C3orf18  
Affinity Capture-MS Homo sapiens
49 Nedd1  
Affinity Capture-MS Mus musculus
50 MOCS3 27304
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 HS1BP3 64342
Affinity Capture-MS Homo sapiens
52 Kif1c  
Affinity Capture-MS Mus musculus
53 CCT8L2  
Affinity Capture-MS Homo sapiens
54 EPHA1 2041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 DYNLL1 8655
Affinity Capture-MS Homo sapiens
56 Exosc1  
Affinity Capture-MS Mus musculus
57 FAM160B2 64760
Affinity Capture-MS Homo sapiens
58 KIAA1919  
Affinity Capture-MS Homo sapiens
59 PAXIP1  
Two-hybrid Homo sapiens
View the network image/svg+xml



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