Gene description for Kif5b
Gene name kinesin family member 5B
Gene symbol Kif5b
Other names/aliases AL022807
Khc
Khcs
Kns1
Ukhc
Species Mus musculus
 Database cross references - Kif5b
ExoCarta ExoCarta_16573
Vesiclepedia VP_16573
Entrez Gene 16573
UniProt Q61768  
 Kif5b identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Kif5b
Molecular Function
    microtubule motor activity GO:0003777 ISO
    microtubule motor activity GO:0003777 ISS
    microtubule motor activity GO:0003777 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    microtubule binding GO:0008017 IBA
    microtubule binding GO:0008017 ISO
    microtubule binding GO:0008017 ISS
    JUN kinase binding GO:0008432 ISO
    plus-end-directed microtubule motor activity GO:0008574 IBA
    ATP hydrolysis activity GO:0016887 IBA
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    protein-containing complex binding GO:0044877 IDA
    microtubule lateral binding GO:0099609 ISO
Biological Process
    mitochondrial transport GO:0006839 TAS
    microtubule-based process GO:0007017 TAS
    cytoplasm organization GO:0007028 IMP
    axon guidance GO:0007411 IBA
    positive regulation of vesicle fusion GO:0031340 ISO
    positive regulation of synaptic transmission, GABAergic GO:0032230 IDA
    lysosome localization GO:0032418 ISO
    lysosome localization GO:0032418 ISS
    stress granule disassembly GO:0035617 IMP
    positive regulation of insulin secretion involved in cellular response to glucose stimulus GO:0035774 ISO
    natural killer cell mediated cytotoxicity GO:0042267 ISO
    natural killer cell mediated cytotoxicity GO:0042267 ISS
    regulation of membrane potential GO:0042391 IEA
    regulation of membrane potential GO:0042391 ISO
    positive regulation of potassium ion transport GO:0043268 IEA
    positive regulation of potassium ion transport GO:0043268 ISO
    vesicle transport along microtubule GO:0047496 IGI
    vesicle transport along microtubule GO:0047496 ISO
    vesicle transport along microtubule GO:0047496 ISS
    mitochondrion transport along microtubule GO:0047497 IMP
    synaptic vesicle transport GO:0048489 IBA
    centrosome localization GO:0051642 ISO
    centrosome localization GO:0051642 ISS
    cellular response to type II interferon GO:0071346 IDA
    plus-end-directed vesicle transport along microtubule GO:0072383 IMP
    positive regulation of intracellular protein transport GO:0090316 ISO
    anterograde dendritic transport of neurotransmitter receptor complex GO:0098971 IBA
    regulation of modification of synapse structure, modulating synaptic transmission GO:0098987 IDA
    regulation of modification of synapse structure, modulating synaptic transmission GO:0098987 IMP
    anterograde axonal protein transport GO:0099641 ISO
    anterograde axonal protein transport GO:0099641 ISS
    mitocytosis GO:0160040 IMP
    positive regulation of protein localization to plasma membrane GO:1903078 IEA
    positive regulation of protein localization to plasma membrane GO:1903078 ISO
    retrograde neuronal dense core vesicle transport GO:1990049 ISO
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 IDA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    kinesin complex GO:0005871 IBA
    kinesin complex GO:0005871 TAS
    microtubule GO:0005874 IBA
    microtubule GO:0005874 IEA
    microtubule cytoskeleton GO:0015630 ISO
    endocytic vesicle GO:0030139 IDA
    vesicle GO:0031982 ISO
    vesicle GO:0031982 ISS
    centriolar satellite GO:0034451 IEA
    centriolar satellite GO:0034451 ISO
    ciliary rootlet GO:0035253 IDA
    neuron projection GO:0043005 IDA
    membrane-bounded organelle GO:0043227 IDA
    axonal growth cone GO:0044295 ISO
    phagocytic vesicle GO:0045335 IDA
    perinuclear region of cytoplasm GO:0048471 ISO
    perinuclear region of cytoplasm GO:0048471 ISS
    postsynaptic cytosol GO:0099524 IDA
    postsynaptic cytosol GO:0099524 IEP
    postsynaptic cytosol GO:0099524 IMP
    cytolytic granule membrane GO:0101004 IEA
    axon cytoplasm GO:1904115 IEA
 Experiment description of studies that identified Kif5b in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Kif5b
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tecpr1  
Co-fractionation Mus musculus
2 Tnik  
Affinity Capture-MS Mus musculus
3 Slc2a4  
Affinity Capture-Western Mus musculus
4 KLC2 64837
Affinity Capture-MS Homo sapiens
5 COPS2 9318
Affinity Capture-MS Homo sapiens
6 Stau1  
Co-fractionation Mus musculus
7 KIDINS220 57498
Affinity Capture-MS Homo sapiens
8 Daxx  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
9 FMR1 2332
Affinity Capture-MS Homo sapiens
10 KLC3  
Affinity Capture-MS Homo sapiens
11 XPR1 9213
Affinity Capture-MS Homo sapiens
12 Fmr1  
Affinity Capture-MS Mus musculus
13 Kctd13  
Affinity Capture-MS Mus musculus
14 MTNR1A  
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
15 Snap25 20614
Affinity Capture-MS Mus musculus
16 KLC1 3831
Affinity Capture-MS Homo sapiens
17 Kcnc1  
FRET Mus musculus
18 LYSMD2  
Affinity Capture-MS Homo sapiens
19 Ywhaz 22631
Affinity Capture-MS Mus musculus
20 KLC4 89953
Affinity Capture-MS Homo sapiens
21 Kif5a  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
22 Herc2  
Affinity Capture-MS Mus musculus
23 Tmem173  
Proximity Label-MS Mus musculus
24 Clcn7  
Affinity Capture-Western Mus musculus
25 Kif5b 16573
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
26 SUN1 23353
Affinity Capture-MS Homo sapiens
27 Kif5c  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
28 ARHGAP11A  
Affinity Capture-MS Homo sapiens
29 Eed  
Affinity Capture-MS Mus musculus
30 Ptpn23 104831
Co-fractionation Mus musculus
31 Ywhae 22627
Affinity Capture-MS Mus musculus
32 VPS8 23355
Affinity Capture-MS Homo sapiens
33 Fancd2  
Affinity Capture-MS Mus musculus
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