Gene description for Snap25
Gene name synaptosomal-associated protein 25
Gene symbol Snap25
Other names/aliases Bdr
GENA70
SNAP-25
SUP
sp
Species Mus musculus
 Database cross references - Snap25
ExoCarta ExoCarta_20614
Vesiclepedia VP_20614
Entrez Gene 20614
UniProt P60879  
 Snap25 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Snap25
Molecular Function
    SNARE binding GO:0000149 IDA
    SNARE binding GO:0000149 ISO
    voltage-gated potassium channel activity GO:0005249 IEA
    voltage-gated potassium channel activity GO:0005249 ISO
    SNAP receptor activity GO:0005484 IBA
    SNAP receptor activity GO:0005484 IDA
    protein binding GO:0005515 IPI
    lipid binding GO:0008289 ISO
    myosin binding GO:0017022 IEA
    myosin binding GO:0017022 ISO
    syntaxin-1 binding GO:0017075 IBA
    syntaxin-1 binding GO:0017075 IDA
    syntaxin-1 binding GO:0017075 ISO
    protein domain specific binding GO:0019904 IEA
    protein domain specific binding GO:0019904 ISO
    transmembrane transporter binding GO:0044325 IEA
    transmembrane transporter binding GO:0044325 ISO
    calcium-dependent protein binding GO:0048306 ISO
    calcium-dependent protein binding GO:0048306 ISS
Biological Process
    exocytosis GO:0006887 IBA
    neurotransmitter secretion GO:0007269 IMP
    axonogenesis GO:0007409 IEA
    long-term memory GO:0007616 IEA
    long-term memory GO:0007616 ISO
    locomotory behavior GO:0007626 IMP
    associative learning GO:0008306 IMP
    regulation of neuron projection development GO:0010975 IGI
    regulation of neuron projection development GO:0010975 ISO
    synaptic vesicle exocytosis GO:0016079 IDA
    synaptic vesicle exocytosis GO:0016079 IMP
    synaptic vesicle exocytosis GO:0016079 ISO
    synaptic vesicle priming GO:0016082 IBA
    endosomal transport GO:0016197 IEA
    endosomal transport GO:0016197 ISO
    insulin secretion GO:0030073 ISO
    synaptic vesicle fusion to presynaptic active zone membrane GO:0031629 IBA
    positive regulation of insulin secretion GO:0032024 IEA
    SNARE complex assembly GO:0035493 IEA
    SNARE complex assembly GO:0035493 ISO
    positive regulation of hormone secretion GO:0046887 ISO
    calcium ion-regulated exocytosis of neurotransmitter GO:0048791 ISO
    establishment of localization in cell GO:0051649 ISO
    regulation of synapse assembly GO:0051963 IEA
    long-term synaptic potentiation GO:0060291 IMP
    regulation of establishment of protein localization GO:0070201 IMP
    potassium ion transmembrane transport GO:0071805 IEA
    presynaptic dense core vesicle exocytosis GO:0099525 IDA
    presynaptic dense core vesicle exocytosis GO:0099525 IEP
    presynaptic dense core vesicle exocytosis GO:0099525 IMP
Subcellular Localization
    photoreceptor inner segment GO:0001917 IEA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    endosome GO:0005768 IEA
    endosome GO:0005768 ISO
    trans-Golgi network GO:0005802 IDA
    cytoskeleton GO:0005856 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 TAS
    cell cortex GO:0005938 IDA
    synaptic vesicle GO:0008021 IDA
    voltage-gated potassium channel complex GO:0008076 ISO
    voltage-gated potassium channel complex GO:0008076 ISS
    actin cytoskeleton GO:0015629 IEA
    actin cytoskeleton GO:0015629 ISO
    membrane GO:0016020 IDA
    lamellipodium GO:0030027 IEA
    lamellipodium GO:0030027 ISO
    filopodium GO:0030175 IEA
    filopodium GO:0030175 ISO
    axon GO:0030424 IDA
    axon GO:0030424 ISO
    growth cone GO:0030426 ISO
    BLOC-1 complex GO:0031083 ISO
    BLOC-1 complex GO:0031083 ISS
    SNARE complex GO:0031201 ISO
    SNARE complex GO:0031201 ISS
    vesicle GO:0031982 ISO
    somatodendritic compartment GO:0036477 IDA
    presynaptic membrane GO:0042734 IDA
    presynaptic membrane GO:0042734 ISO
    neuron projection GO:0043005 IDA
    neuron projection GO:0043005 ISO
    neuronal cell body GO:0043025 IEA
    neuronal cell body GO:0043025 ISO
    myelin sheath GO:0043209 HDA
    axonal growth cone GO:0044295 ISO
    synapse GO:0045202 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    presynaptic active zone membrane GO:0048787 ISO
    synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex GO:0070032 IBA
    synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex GO:0070032 IDA
    synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex GO:0070032 ISO
    synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex GO:0070033 IEA
    synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex GO:0070033 ISO
    synaptobrevin 2-SNAP-25-syntaxin-1a complex GO:0070044 ISO
    ribbon synapse GO:0097470 IDA
    extrinsic component of presynaptic membrane GO:0098888 IEA
    extrinsic component of presynaptic membrane GO:0098888 ISO
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Snap25 in exosomes
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Snap25
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tubb2a  
Affinity Capture-MS Mus musculus
2 Tuba4a 22145
Affinity Capture-MS Mus musculus
3 Snap47  
Co-fractionation Mus musculus
4 Scamp1  
Co-fractionation Mus musculus
5 Stx2 25130
Two-hybrid Rattus norvegicus
Two-hybrid Rattus norvegicus
6 Cox6b1  
Co-fractionation Mus musculus
7 Stx1a  
Two-hybrid Rattus norvegicus
Two-hybrid Rattus norvegicus
8 Dnajc5 13002
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
9 Pcsk2  
Co-fractionation Mus musculus
10 Hspa8 15481
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
11 Aldoc 11676
Co-fractionation Mus musculus
12 Snapin  
Co-fractionation Mus musculus
13 Tubb5 22154
Affinity Capture-MS Mus musculus
14 Stx4 81803
Two-hybrid Rattus norvegicus
Two-hybrid Rattus norvegicus
15 Gpm6a  
Co-fractionation Mus musculus
16 Cntn1 12805
Co-fractionation Mus musculus
17 Stx1a  
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
18 Atp1a1 11928
Affinity Capture-MS Mus musculus
19 Gapdh 14433
Affinity Capture-MS Mus musculus
20 Uqcrh  
Co-fractionation Mus musculus
21 Map6  
Affinity Capture-MS Mus musculus
22 Stx3  
Two-hybrid Rattus norvegicus
Two-hybrid Rattus norvegicus
23 Pacs1 107975
Affinity Capture-MS Mus musculus
24 Ap2b1 71770
Affinity Capture-MS Mus musculus
25 Stx12  
Co-fractionation Mus musculus
26 Zfhx3 11906
Affinity Capture-MS Mus musculus
27 Tuba1a 22142
Affinity Capture-MS Mus musculus
28 MTNR1A  
Reconstituted Complex Homo sapiens
29 Htt  
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
30 Sgta 52551
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
31 Napb  
Co-fractionation Mus musculus
32 Nckap1 50884
Affinity Capture-MS Mus musculus
33 Atp6v0d1 11972
Affinity Capture-MS Mus musculus
34 Hspg2 15530
Affinity Capture-MS Mus musculus
35 Gnao1 14681
Co-fractionation Mus musculus
36 Kif5b 16573
Affinity Capture-MS Mus musculus
37 Usp48  
Affinity Capture-MS Mus musculus
38 MAPT  
Reconstituted Complex Homo sapiens
39 Cenpf  
Affinity Capture-Western Mus musculus
40 Plp1 18823
Co-fractionation Mus musculus
41 Syn1  
Co-fractionation Mus musculus
42 Kif5c  
Affinity Capture-MS Mus musculus
43 Hgs 15239
Reconstituted Complex Mus musculus
44 Srcin1  
Co-fractionation Mus musculus
45 Ap2a1 11771
Affinity Capture-MS Mus musculus
46 Syp  
Affinity Capture-MS Mus musculus
Co-fractionation Mus musculus
47 Vamp2  
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
48 Snca  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
49 Sstr3  
Proximity Label-MS Mus musculus
50 Prrt2  
Co-fractionation Mus musculus
Affinity Capture-Western Mus musculus
51 Mapt  
Affinity Capture-MS Mus musculus
52 Tia1  
Affinity Capture-MS Mus musculus
53 Slc6a1  
Co-fractionation Mus musculus
54 Syt1  
Co-fractionation Mus musculus
55 Syt2  
Co-fractionation Mus musculus
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