Gene description for Gnao1
Gene name guanine nucleotide binding protein, alpha O
Gene symbol Gnao1
Other names/aliases AW050213
Galphao
Gnao
alphaO
Species Mus musculus
 Database cross references - Gnao1
ExoCarta ExoCarta_14681
Entrez Gene 14681
UniProt P18872  
 Gnao1 identified in exosomes derived from the following tissue/cell type
Embryonic fibroblasts 18494037    
Pancreatic cells 19351151    
 Gene ontology annotations for Gnao1
Molecular Function
    G-protein coupled serotonin receptor binding GO:0031821 ISO
    signal transducer activity GO:0004871 IBA
    corticotropin-releasing hormone receptor 1 binding GO:0051430 ISO
    protein complex binding GO:0032403 ISO
    GTP binding GO:0005525 ISO
    mu-type opioid receptor binding GO:0031852 ISO
    metal ion binding GO:0046872 IEA
    guanyl nucleotide binding GO:0019001 IEA
    nucleotide binding GO:0000166 IEA
    G-protein beta/gamma-subunit complex binding GO:0031683 IBA
    GTPase activity GO:0003924 TAS
    protein binding GO:0005515 IPI
    GTPase activating protein binding GO:0032794 ISO
Biological Process
    response to organonitrogen compound GO:0010243 ISO
    signal transduction GO:0007165 IEA
    regulation of heart contraction GO:0008016 IMP
    locomotory behavior GO:0007626 IMP
    positive regulation of GTPase activity GO:0043547 ISO
    G-protein coupled receptor signaling pathway GO:0007186 TAS
    adenylate cyclase-modulating G-protein coupled receptor signaling pathway GO:0007188 IBA
    metabolic process GO:0008152 ISO
    dopamine receptor signaling pathway GO:0007212 IMP
    negative regulation of calcium ion transport GO:0051926 ISO
    aging GO:0007568 ISO
    cellular process GO:0009987 IMP
Subcellular Localization
    neuron projection GO:0043005 ISO
    dendrite GO:0030425 IDA
    intracellular GO:0005622 IDA
    plasma membrane GO:0005886 IEA
    myelin sheath GO:0043209 ISO
    membrane GO:0016020 TAS
    heterotrimeric G-protein complex GO:0005834 TAS
    protein complex GO:0043234 ISO
 Experiment description of studies that identified Gnao1 in exosomes
1
Experiment ID 73
ISEV standards
EM
EV Biophysical techniques
HSP70|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
2
Experiment ID 188
ISEV standards
EM
EV Biophysical techniques
GAPDH|UCHL1|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Gnao1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Gnao1 is involved
PathwayEvidenceSource
Ca2+ pathway IEA Reactome
G-protein activation IEA Reactome
PLC beta mediated events IEA Reactome





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