Gene description for Stx4
Gene name syntaxin 4
Gene symbol Stx4
Other names/aliases Stx4a
Species Rattus norvegicus
 Database cross references - Stx4
ExoCarta ExoCarta_81803
Vesiclepedia VP_81803
Entrez Gene 81803
UniProt Q08850  
 Stx4 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Stx4
Molecular Function
    SNARE binding GO:0000149 IBA
    SNARE binding GO:0000149 IPI
    SNARE binding GO:0000149 ISO
    SNAP receptor activity GO:0005484 IBA
    SNAP receptor activity GO:0005484 IDA
    SNAP receptor activity GO:0005484 IEA
    protein binding GO:0005515 IPI
    sphingomyelin phosphodiesterase activator activity GO:0016230 ISO
    small GTPase binding GO:0031267 IPI
Biological Process
    positive regulation of immunoglobulin production GO:0002639 ISO
    intracellular protein transport GO:0006886 IBA
    intracellular protein transport GO:0006886 IEA
    exocytosis GO:0006887 IBA
    vesicle fusion GO:0006906 IBA
    sensory perception of sound GO:0007605 ISO
    sensory perception of sound GO:0007605 ISS
    positive regulation of cell population proliferation GO:0008284 ISO
    synaptic vesicle docking GO:0016081 IDA
    vesicle-mediated transport GO:0016192 IEA
    regulation of exocytosis GO:0017157 ISO
    positive regulation of cell migration GO:0030335 ISO
    protein localization to cell surface GO:0034394 IMP
    cellular response to oxidative stress GO:0034599 ISO
    SNARE complex assembly GO:0035493 ISO
    positive regulation of insulin secretion involved in cellular response to glucose stimulus GO:0035774 ISO
    positive regulation of catalytic activity GO:0043085 ISO
    positive regulation of eosinophil degranulation GO:0043311 ISO
    positive regulation of cell adhesion GO:0045785 ISO
    intracellular transport GO:0046907 NAS
    vesicle docking GO:0048278 IBA
    organelle fusion GO:0048284 ISO
    positive regulation of chemotaxis GO:0050921 ISO
    long-term synaptic potentiation GO:0060291 ISO
    membrane fusion GO:0061025 IDA
    membrane fusion GO:0061025 IEA
    protein-containing complex assembly GO:0065003 IDA
    cellular response to type II interferon GO:0071346 ISO
    exocytic insertion of neurotransmitter receptor to postsynaptic membrane GO:0098967 IDA
    exocytic insertion of neurotransmitter receptor to postsynaptic membrane GO:0098967 IMP
    vesicle-mediated transport in synapse GO:0099003 IDA
    vesicle-mediated transport in synapse GO:0099003 IMP
    regulation of postsynaptic membrane neurotransmitter receptor levels GO:0099072 ISO
    regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902041 ISO
    positive regulation of protein localization to plasma membrane GO:1903078 ISO
    cornified envelope assembly GO:1903575 ISO
    positive regulation of protein localization to cell surface GO:2000010 ISO
Subcellular Localization
    storage vacuole GO:0000322 ISO
    extracellular space GO:0005615 ISO
    cytoplasm GO:0005737 ISO
    endosome GO:0005768 ISO
    trans-Golgi network GO:0005802 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISO
    cell surface GO:0009986 ISO
    endomembrane system GO:0012505 IBA
    membrane GO:0016020 IEA
    membrane GO:0016020 ISO
    basolateral plasma membrane GO:0016323 IDA
    basolateral plasma membrane GO:0016323 ISO
    apical plasma membrane GO:0016324 IDA
    lamellipodium GO:0030027 ISO
    SNARE complex GO:0031201 IBA
    SNARE complex GO:0031201 IDA
    SNARE complex GO:0031201 ISO
    stereocilium GO:0032420 ISO
    stereocilium GO:0032420 ISS
    neuron projection membrane GO:0032589 IDA
    myelin sheath adaxonal region GO:0035749 ISO
    somatodendritic compartment GO:0036477 ISO
    sarcolemma GO:0042383 IDA
    specific granule GO:0042581 ISO
    dendritic spine GO:0043197 ISO
    lateral loop GO:0043219 ISO
    synapse GO:0045202 ISO
    postsynaptic membrane GO:0045211 IDA
    phagocytic vesicle GO:0045335 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    Schaffer collateral - CA1 synapse GO:0098685 IDA
    Schaffer collateral - CA1 synapse GO:0098685 IMP
    Schaffer collateral - CA1 synapse GO:0098685 ISO
    presynapse GO:0098793 IEA
    postsynapse GO:0098794 ISO
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Stx4 in exosomes
1
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Stx4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Snap25 20614
Two-hybrid Mus musculus
Two-hybrid Mus musculus
2 Stxbp1 25558
Reconstituted Complex Rattus norvegicus
View the network image/svg+xml



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