ExoCarta: Gene summary

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Gene description for Stx4
Gene name	syntaxin 4
Gene symbol	Stx4
Other names/aliases	Stx4a
Species	Rattus norvegicus
Database cross references - Stx4
ExoCarta	ExoCarta_81803
Entrez Gene	81803
UniProt	Q08850
Stx4 identified in exosomes derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Stx4

Molecular Function
	protein binding	GO:0005515	IPI
	SNAP receptor activity	GO:0005484	IEA
	sphingomyelin phosphodiesterase activator activity	GO:0016230	ISO
	Rab GTPase binding	GO:0017137	IPI
	SNARE binding	GO:0000149	ISO
	spectrin binding	GO:0030507	IPI
Biological Process
	response to hydroperoxide	GO:0033194	ISO
	positive regulation of eosinophil degranulation	GO:0043311	ISO
	positive regulation of cell migration	GO:0030335	ISO
	protein complex assembly	GO:0006461	IDA
	positive regulation of catalytic activity	GO:0043085	ISO
	regulation of exocytosis	GO:0017157	ISO
	positive regulation of cell proliferation	GO:0008284	ISO
	positive regulation of establishment of protein localization to plasma membrane	GO:0090004	ISO
	long-term synaptic potentiation	GO:0060291	ISO
	intracellular protein transport	GO:0006886	IEA
	positive regulation of insulin secretion involved in cellular response to glucose stimulus	GO:0035774	ISO
	SNARE complex assembly	GO:0035493	ISO
	exocytosis	GO:0006887	IBA
	organelle fusion	GO:0048284	ISO
	positive regulation of cell adhesion	GO:0045785	ISO
	positive regulation of protein localization to cell surface	GO:2000010	ISO
	synaptic vesicle docking	GO:0016081	IDA
	membrane fusion	GO:0061025	IDA
	positive regulation of protein localization to plasma membrane	GO:1903078	ISO
	positive regulation of chemotaxis	GO:0050921	ISO
	positive regulation of immunoglobulin secretion	GO:0051024	ISO
	intracellular transport	GO:0046907	NAS
	synaptic vesicle fusion to presynaptic membrane	GO:0031629	IBA
	regulation of extrinsic apoptotic signaling pathway via death domain receptors	GO:1902041	ISO
Subcellular Localization
	storage vacuole	GO:0000322	ISO
	myelin sheath adaxonal region	GO:0035749	ISO
	specific granule	GO:0042581	ISO
	lateral loop	GO:0043219	ISO
	sarcolemma	GO:0042383	IDA
	SNARE complex	GO:0031201	ISO
	somatodendritic compartment	GO:0036477	ISO
	endomembrane system	GO:0012505	IBA
	perinuclear region of cytoplasm	GO:0048471	ISO
	basolateral plasma membrane	GO:0016323	ISO
	plasma membrane	GO:0005886	ISO
	lamellipodium	GO:0030027	ISO
	trans-Golgi network	GO:0005802	ISO
	cytoplasm	GO:0005737	ISO
	membrane	GO:0016020	ISO
	synaptic vesicle	GO:0008021	IBA
	synapse	GO:0045202	ISO
	apical plasma membrane	GO:0016324	IDA
	integral component of membrane	GO:0016021	IBA
	intracellular	GO:0005622	ISO
	extracellular space	GO:0005615	ISO
	cell surface	GO:0009986	ISO
	endosome	GO:0005768	ISO
	extracellular exosome	GO:0070062	ISO
	dendritic spine	GO:0043197	ISO

Experiment description of studies that identified Stx4 in exosomes

Experiment ID

ISEV standards
✔ EM	EV Biophysical techniques
✔ TSG101\|HSP90\|HSC70	EV Cytosolic markers
✔ MHCI	EV Membrane markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D2 Rep 2

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.16-1.21 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting