Gene description for HBA2
Gene name hemoglobin, alpha 2
Gene symbol HBA2
Other names/aliases HBA-T2
HBH
Species Homo sapiens
 Database cross references - HBA2
ExoCarta ExoCarta_3040
Vesiclepedia VP_3040
Entrez Gene 3040
HGNC 4824
MIM 141850
UniProt P69905  
 HBA2 identified in exosomes derived from the following tissue/cell type
Bladder cancer cells 20224111    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Plasma 19028452    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Thymus 23844026    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for HBA2
Molecular Function
    G protein-coupled receptor binding GO:0001664 IDA
    peroxidase activity GO:0004601 IBA
    peroxidase activity GO:0004601 IDA
    oxygen carrier activity GO:0005344 IBA
    iron ion binding GO:0005506 IEA
    protein binding GO:0005515 IPI
    oxygen binding GO:0019825 IBA
    heme binding GO:0020037 IBA
    haptoglobin binding GO:0031720 IBA
    haptoglobin binding GO:0031720 IDA
Biological Process
    carbon dioxide transport GO:0015670 NAS
    oxygen transport GO:0015671 IDA
    oxygen transport GO:0015671 IMP
    oxygen transport GO:0015671 NAS
    oxygen transport GO:0015671 TAS
    nitric oxide transport GO:0030185 IDA
    response to hydrogen peroxide GO:0042542 IDA
    hydrogen peroxide catabolic process GO:0042744 IBA
    hydrogen peroxide catabolic process GO:0042744 IDA
    cellular oxidant detoxification GO:0098869 IEA
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IDA
    cytosol GO:0005829 TAS
    hemoglobin complex GO:0005833 IBA
    hemoglobin complex GO:0005833 IDA
    hemoglobin complex GO:0005833 IPI
    hemoglobin complex GO:0005833 TAS
    membrane GO:0016020 HDA
    haptoglobin-hemoglobin complex GO:0031838 IBA
    haptoglobin-hemoglobin complex GO:0031838 IDA
    extracellular exosome GO:0070062 HDA
    endocytic vesicle lumen GO:0071682 TAS
    blood microparticle GO:0072562 HDA
 Experiment description of studies that identified HBA2 in exosomes
1
Experiment ID 76
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
2
Experiment ID 282
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
3
Experiment ID 283
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
4
Experiment ID 285
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 44
MISEV standards
✔
IEM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19028452    
Organism Homo sapiens
Experiment description Proteomic profiling of human plasma exosomes identifies PPARgamma as an exosome-associated protein.
Authors "Looze C, Yui D, Leung L, Ingham M, Kaler M, Yao X, Wu WW, Shen RF, Daniels MP, Levine SJ"
Journal name BBRC
Publication year 2009
Sample Plasma
Sample name Plasma
Isolation/purification methods Filtration
Size exclusion
Sucrose density gradient
Flotation density 1.15-1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
11
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
14
Experiment ID 196
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
15
Experiment ID 197
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for HBA2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TRAF3IP3  
Affinity Capture-MS Homo sapiens
2 CSTL1  
Affinity Capture-MS Homo sapiens
3 HBB 3043
Two-hybrid Homo sapiens
4 HBD 3045
Two-hybrid Homo sapiens
5 MACROD1 28992
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 SSR2  
Affinity Capture-MS Homo sapiens
7 HBE1 3046
Two-hybrid Homo sapiens
8 UGT2A3  
Affinity Capture-MS Homo sapiens
9 ACAA1 30
Affinity Capture-MS Homo sapiens
10 LPAR6  
Affinity Capture-MS Homo sapiens
11 ALB 213
Affinity Capture-MS Homo sapiens
12 FGF4  
Affinity Capture-MS Homo sapiens
13 HIST1H2BJ 8970
Affinity Capture-MS Homo sapiens
14 AGA 175
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 RIC8A 60626
Affinity Capture-MS Homo sapiens
16 GBP2  
Affinity Capture-MS Homo sapiens
17 C9orf85  
Affinity Capture-MS Homo sapiens
18 IGSF8 93185
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 NUFIP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 KRT40  
Two-hybrid Homo sapiens
21 OBSL1 23363
Affinity Capture-MS Homo sapiens
22 RER1 11079
Affinity Capture-MS Homo sapiens
23 ALK 238
Affinity Capture-MS Homo sapiens
24 ITGA4 3676
Affinity Capture-MS Homo sapiens
25 DGCR2 9993
Affinity Capture-MS Homo sapiens
26 UCHL5 51377
Reconstituted Complex Homo sapiens
27 MSL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 VCAM1 7412
Affinity Capture-MS Homo sapiens
29 FCGR1A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 SDCBP 6386
Affinity Capture-MS Homo sapiens
31 HSPA8 3312
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 UBE2V1 7335
Affinity Capture-MS Homo sapiens
33 KRT34  
Two-hybrid Homo sapiens
34 MANSC1 54682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 C1orf228  
Affinity Capture-MS Homo sapiens
36 TSPAN33 340348
Affinity Capture-MS Homo sapiens
37 PLXNB1 5364
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 PARK2  
Affinity Capture-MS Homo sapiens
39 PNKP 11284
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 DHRS2 10202
Affinity Capture-MS Homo sapiens
41 NDUFAF5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 TAF12  
Affinity Capture-MS Homo sapiens
43 NXPH2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 GPR182  
Affinity Capture-MS Homo sapiens
45 RLIM 51132
Affinity Capture-MS Homo sapiens
46 DNAJA1 3301
Affinity Capture-MS Homo sapiens
47 ARHGEF6 9459
Affinity Capture-MS Homo sapiens
48 KLK10 5655
Affinity Capture-MS Homo sapiens
49 GIP  
Affinity Capture-MS Homo sapiens
50 DKK2  
Affinity Capture-MS Homo sapiens
51 SDHD  
Affinity Capture-MS Homo sapiens
52 NOTCH2NL 388677
Two-hybrid Homo sapiens
53 RPL32 6161
Affinity Capture-MS Homo sapiens
54 HBG2 3048
Two-hybrid Homo sapiens
55 HMGCLL1  
Affinity Capture-MS Homo sapiens
56 TNFSF8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 CCDC57 284001
Two-hybrid Homo sapiens
58 IQCB1  
Affinity Capture-MS Homo sapiens
59 GADD45B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 CD28  
Affinity Capture-MS Homo sapiens
61 EGFR 1956
Affinity Capture-MS Homo sapiens
62 HSD17B10 3028
Affinity Capture-MS Homo sapiens
63 UPF3A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 PLLP  
Affinity Capture-MS Homo sapiens
65 MAPK6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 OPTC  
Affinity Capture-MS Homo sapiens
67 SLC2A14 144195
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 DLST 1743
Affinity Capture-MS Homo sapiens
69 PXK 54899
Affinity Capture-MS Homo sapiens
70 GSTT1 2952
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 FN1 2335
Affinity Capture-MS Homo sapiens
72 SCGB1D1  
Affinity Capture-MS Homo sapiens
73 FCGRT 2217
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 SPP1 6696
Two-hybrid Homo sapiens
75 PGPEP1 54858
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 GDPD1 284161
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 NBPF19  
Two-hybrid Homo sapiens
78 LATS1  
Affinity Capture-MS Homo sapiens
79 FBLN7  
Affinity Capture-MS Homo sapiens
80 HLA-DPA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 STMN3  
Affinity Capture-MS Homo sapiens
82 KLHL22 84861
Affinity Capture-MS Homo sapiens
83 CUL7 9820
Affinity Capture-MS Homo sapiens
84 DTWD1  
Affinity Capture-MS Homo sapiens
85 NUP210P1  
Affinity Capture-MS Homo sapiens
86 MATN2 4147
Affinity Capture-MS Homo sapiens
87 C1QL3  
Affinity Capture-MS Homo sapiens
88 P2RX6  
Affinity Capture-MS Homo sapiens
89 CXCL16 58191
Affinity Capture-MS Homo sapiens
90 DKKL1  
Affinity Capture-MS Homo sapiens
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