Gene description for CEPT1
Gene name choline/ethanolamine phosphotransferase 1
Gene symbol CEPT1
Other names/aliases -
Species Homo sapiens
 Database cross references - CEPT1
ExoCarta ExoCarta_10390
Vesiclepedia VP_10390
Entrez Gene 10390
HGNC 24289
UniProt Q9Y6K0  
 CEPT1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for CEPT1
Molecular Function
    diacylglycerol cholinephosphotransferase activity GO:0004142 IBA
    diacylglycerol cholinephosphotransferase activity GO:0004142 IDA
    ethanolaminephosphotransferase activity GO:0004307 IBA
    ethanolaminephosphotransferase activity GO:0004307 IDA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    1-alkenyl-2-acylglycerol choline phosphotransferase activity GO:0047359 IEA
Biological Process
    lipid metabolic process GO:0006629 TAS
    phosphatidylethanolamine biosynthetic process GO:0006646 IBA
    phosphatidylethanolamine biosynthetic process GO:0006646 IDA
    phosphatidylethanolamine biosynthetic process GO:0006646 IEA
    phosphatidylcholine biosynthetic process GO:0006656 IDA
    CDP-choline pathway GO:0006657 IEA
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 IBA
    endoplasmic reticulum membrane GO:0005789 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
    Golgi apparatus GO:0005794 IBA
    membrane GO:0016020 TAS
    nuclear membrane GO:0031965 IEA
 Experiment description of studies that identified CEPT1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for CEPT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KDF1  
Affinity Capture-MS Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 TOMM40 10452
Affinity Capture-MS Homo sapiens
4 Tor1aip1  
Affinity Capture-MS Mus musculus
5 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
6 FASN 2194
Negative Genetic Homo sapiens
7 TP53 7157
Affinity Capture-MS Homo sapiens
8 Mtx2  
Affinity Capture-MS Mus musculus
9 VAPA 9218
Affinity Capture-MS Homo sapiens
10 DCP1B  
Affinity Capture-MS Homo sapiens
11 PGRMC1 10857
Affinity Capture-MS Homo sapiens
12 UGGT1 56886
Affinity Capture-MS Homo sapiens
13 Kifc1  
Affinity Capture-MS Mus musculus
14 KRT8 3856
Proximity Label-MS Homo sapiens
15 ARFGAP2 84364
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 MYH14 79784
Affinity Capture-MS Homo sapiens
17 TMEM14B  
Two-hybrid Homo sapiens
18 SMC6  
Affinity Capture-MS Homo sapiens
19 EMD 2010
Proximity Label-MS Homo sapiens
20 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
21 TMEM263 90488
Affinity Capture-MS Homo sapiens
22 KLRB1  
Affinity Capture-MS Homo sapiens
23 RBM6 10180
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 Rock1  
Affinity Capture-MS Mus musculus
25 YIPF5 81555
Affinity Capture-MS Homo sapiens
26 SOX2  
Affinity Capture-MS Homo sapiens
27 PSMC1 5700
Affinity Capture-MS Homo sapiens
28 RPL5 6125
Affinity Capture-MS Homo sapiens
29 CDC42 998
Affinity Capture-MS Homo sapiens
30 WDR62  
Affinity Capture-MS Homo sapiens
31 CAPZB 832
Affinity Capture-MS Homo sapiens
32 MAPK6  
Affinity Capture-MS Homo sapiens
33 QSOX2 169714
Affinity Capture-MS Homo sapiens
34 MYC  
Affinity Capture-MS Homo sapiens
35 KIF22  
Affinity Capture-MS Homo sapiens
36 CYLD  
Affinity Capture-MS Homo sapiens
37 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
38 RAB9A 9367
Proximity Label-MS Homo sapiens
39 Chmp4c  
Affinity Capture-MS Mus musculus
40 CHST15 51363
Affinity Capture-MS Homo sapiens
41 ACAD9 28976
Proximity Label-MS Homo sapiens
42 IQGAP1 8826
Affinity Capture-MS Homo sapiens
43 Prmt6  
Affinity Capture-MS Mus musculus
44 OAZ1 4946
Affinity Capture-MS Homo sapiens
45 C9orf72  
Affinity Capture-MS Homo sapiens
46 XRCC3  
Affinity Capture-MS Homo sapiens
47 MLLT4 4301
Affinity Capture-MS Homo sapiens
48 MARCH5  
Proximity Label-MS Homo sapiens
49 PEX3 8504
Proximity Label-MS Homo sapiens
50 AKAP1 8165
Proximity Label-MS Homo sapiens
51 SEC61B 10952
Proximity Label-MS Homo sapiens
52 SLC33A1 9197
Affinity Capture-MS Homo sapiens
53 EXD2  
Proximity Label-MS Homo sapiens
54 Cdc26  
Affinity Capture-MS Mus musculus
55 CCDC47 57003
Affinity Capture-MS Homo sapiens
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