Gene description for INPP5B
Gene name inositol polyphosphate-5-phosphatase, 75kDa
Gene symbol INPP5B
Other names/aliases 5PTase
Species Homo sapiens
 Database cross references - INPP5B
ExoCarta ExoCarta_3633
Vesiclepedia VP_3633
Entrez Gene 3633
HGNC 6077
MIM 147264
UniProt P32019  
 INPP5B identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for INPP5B
Molecular Function
    phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity GO:0004439 IBA
    phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity GO:0004439 IDA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    inositol-1,4,5-trisphosphate 5-phosphatase activity GO:0052658 IBA
    inositol-1,4,5-trisphosphate 5-phosphatase activity GO:0052658 IDA
    inositol-1,4,5-trisphosphate 5-phosphatase activity GO:0052658 TAS
    inositol-1,3,4,5-tetrakisphosphate 5-phosphatase activity GO:0052659 TAS
Biological Process
    in utero embryonic development GO:0001701 IEA
    signal transduction GO:0007165 IEA
    spermatogenesis GO:0007283 IEA
    flagellated sperm motility GO:0030317 IEA
    inositol phosphate metabolic process GO:0043647 TAS
    phosphatidylinositol dephosphorylation GO:0046856 IDA
    regulation of protein processing GO:0070613 IEA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 IEA
    Golgi apparatus GO:0005794 IEA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 IBA
    membrane GO:0016020 IDA
    phagocytic vesicle membrane GO:0030670 IEA
    early endosome membrane GO:0031901 IEA
    neuron projection GO:0043005 IBA
 Experiment description of studies that identified INPP5B in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for INPP5B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NUF2  
Affinity Capture-MS Homo sapiens
2 FASN 2194
Negative Genetic Homo sapiens
3 DNAJC11 55735
Affinity Capture-MS Homo sapiens
4 FAM109B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 RAB5A 5868
Proximity Label-MS Homo sapiens
6 Dync1li1 235661
Affinity Capture-MS Mus musculus
7 ATG9A 79065
Proximity Label-MS Homo sapiens
8 Rmdn3  
Affinity Capture-MS Mus musculus
9 PRKCZ 5590
Co-fractionation Homo sapiens
10 KIF20A 10112
Affinity Capture-MS Homo sapiens
11 Shoc2  
Affinity Capture-MS Mus musculus
12 PARK2  
Affinity Capture-MS Homo sapiens
13 FAM109A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 APP 351
Reconstituted Complex Homo sapiens
15 OCRL 4952
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 Tuba3a 22144
Affinity Capture-MS Mus musculus
17 JUN 3725
Affinity Capture-MS Homo sapiens
18 JAK3 3718
Affinity Capture-MS Homo sapiens
19 RNASEH2A 10535
Affinity Capture-MS Homo sapiens
20 PARD6B 84612
Affinity Capture-MS Homo sapiens
21 RAB9A 9367
Proximity Label-MS Homo sapiens
22 Setdb1  
Affinity Capture-MS Mus musculus
23 Clspn  
Affinity Capture-MS Mus musculus
24 BCAT1 586
Co-fractionation Homo sapiens
25 FGFR1OP  
Affinity Capture-MS Homo sapiens
26 Cbx4  
Affinity Capture-MS Mus musculus
27 Cdc26  
Affinity Capture-MS Mus musculus
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