Gene description for WIPF2
Gene name WAS/WASL interacting protein family, member 2
Gene symbol WIPF2
Other names/aliases WICH
WIRE
Species Homo sapiens
 Database cross references - WIPF2
ExoCarta ExoCarta_147179
Vesiclepedia VP_147179
Entrez Gene 147179
HGNC 30923
MIM 609692
UniProt Q8TF74  
 WIPF2 identified in exosomes derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for WIPF2
Molecular Function
    actin binding GO:0003779 IEA
    protein binding GO:0005515 IPI
Subcellular Localization
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IEA
 Experiment description of studies that identified WIPF2 in exosomes
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for WIPF2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPDL 9987
Co-fractionation Homo sapiens
2 ZFAND5 7763
Co-fractionation Homo sapiens
3 SLC4A1AP  
Co-fractionation Homo sapiens
4 EIF4A3 9775
Co-fractionation Homo sapiens
5 NCK1 4690
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 GRB2 2885
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 FBXL6  
Proximity Label-MS Homo sapiens
8 WWOX 51741
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
9 TCERG1 10915
Reconstituted Complex Homo sapiens
10 ACTC1 70
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
11 PACSIN2 11252
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 FGA 2243
Affinity Capture-MS Homo sapiens
13 APBB1  
Reconstituted Complex Homo sapiens
14 DDX58 23586
Affinity Capture-RNA Homo sapiens
15 NCK2 8440
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
16 WAS 7454
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
17 ITSN2 50618
Two-hybrid Homo sapiens
18 TXLNG  
Co-fractionation Homo sapiens
19 CDH1 999
Proximity Label-MS Homo sapiens
20 DNMBP 23268
Affinity Capture-MS Homo sapiens
21 SORBS3 10174
Two-hybrid Homo sapiens
22 ACTB 60
Proximity Label-MS Homo sapiens
23 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
24 TVP23A  
Two-hybrid Homo sapiens
25 ACTG1 71
Affinity Capture-MS Homo sapiens
26 ZNF830  
Co-fractionation Homo sapiens
27 WASL 8976
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here