Gene description for PHACTR1
Gene name phosphatase and actin regulator 1
Gene symbol PHACTR1
Other names/aliases RPEL
RPEL1
dJ257A7.2
Species Homo sapiens
 Database cross references - PHACTR1
ExoCarta ExoCarta_221692
Vesiclepedia VP_221692
Entrez Gene 221692
HGNC 20990
MIM 608723
UniProt Q9C0D0  
 PHACTR1 identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for PHACTR1
Molecular Function
    actin binding GO:0003779 IBA
    actin binding GO:0003779 ISS
    protein phosphatase inhibitor activity GO:0004864 IEA
    protein binding GO:0005515 IPI
Biological Process
    cerebral cortex development GO:0021987 ISS
    actin cytoskeleton organization GO:0030036 ISS
    actomyosin structure organization GO:0031032 IMP
    stress fiber assembly GO:0043149 IBA
    stress fiber assembly GO:0043149 IMP
    cell motility GO:0048870 IBA
    cell motility GO:0048870 IMP
    dendrite arborization GO:0140059 ISS
    regulation of neuron migration GO:2001222 ISS
Subcellular Localization
    nucleus GO:0005634 ISS
    cytosol GO:0005829 ISS
    synapse GO:0045202 IEA
 Experiment description of studies that identified PHACTR1 in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for PHACTR1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 C14orf119  
Two-hybrid Homo sapiens
2 CBX8 57332
Two-hybrid Homo sapiens
3 ACTA1 58
Affinity Capture-Western Homo sapiens
4 LINC00839  
Protein-RNA Homo sapiens
5 PPP1CA 5499
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which PHACTR1 is involved
No pathways found





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