Gene description for ABLIM3
Gene name actin binding LIM protein family, member 3
Gene symbol ABLIM3
Other names/aliases HMFN1661
Species Homo sapiens
 Database cross references - ABLIM3
ExoCarta ExoCarta_22885
Vesiclepedia VP_22885
Entrez Gene 22885
HGNC 29132
MIM 611305
UniProt O94929  
 ABLIM3 identified in sEVs derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for ABLIM3
Molecular Function
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    actin filament binding GO:0051015 IBA
Biological Process
    transcription by RNA polymerase II GO:0006366 IEA
    cytoskeleton organization GO:0007010 IEA
    lamellipodium assembly GO:0030032 IBA
    lamellipodium assembly GO:0030032 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 IEA
    cilium assembly GO:0060271 IBA
    cilium assembly GO:0060271 IMP
    positive regulation of protein targeting to mitochondrion GO:1903955 HMP
Subcellular Localization
    stress fiber GO:0001725 IBA
    stress fiber GO:0001725 IDA
    cytoplasm GO:0005737 IEA
    actin cytoskeleton GO:0015629 IBA
    lamellipodium GO:0030027 IDA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified ABLIM3 in sEVs
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ABLIM3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PDLIM7 9260
Affinity Capture-MS Homo sapiens
2 CCNH  
Affinity Capture-MS Homo sapiens
3 TARBP1 6894
Affinity Capture-MS Homo sapiens
4 ABRA  
Affinity Capture-Western Homo sapiens
5 IKZF3  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
6 BANP  
Two-hybrid Homo sapiens
7 ACTC1 70
Affinity Capture-Western Homo sapiens
8 BRCA1 672
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
9 AGMAT  
Affinity Capture-MS Homo sapiens
10 CWF19L2  
Two-hybrid Homo sapiens
11 C11orf73 51501
Two-hybrid Homo sapiens
12 CCDC33 80125
Two-hybrid Homo sapiens
13 SOX2  
Affinity Capture-MS Homo sapiens
14 ZNF426  
Affinity Capture-MS Homo sapiens
15 APEX1 328
Affinity Capture-RNA Homo sapiens
16 ACTA1 58
Co-purification Homo sapiens
17 ABI2 10152
Two-hybrid Homo sapiens
18 ZNF620  
Two-hybrid Homo sapiens
19 CDH1 999
Proximity Label-MS Homo sapiens
20 MLLT4 4301
Affinity Capture-MS Homo sapiens
21 BBS9 27241
Affinity Capture-MS Homo sapiens
22 CBX8 57332
Two-hybrid Homo sapiens
23 LIMD1 8994
Affinity Capture-MS Homo sapiens
24 SNW1 22938
Two-hybrid Homo sapiens
25 MNAT1  
Affinity Capture-MS Homo sapiens
26 BBS2 583
Affinity Capture-MS Homo sapiens
27 GNPDA1 10007
Affinity Capture-MS Homo sapiens
28 ATRX 546
Affinity Capture-MS Homo sapiens
29 CATSPER1  
Two-hybrid Homo sapiens
30 SSX2IP  
Two-hybrid Homo sapiens
View the network image/svg+xml



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