Gene description for PRKCH
Gene name protein kinase C, eta
Gene symbol PRKCH
Other names/aliases PKC-L
PKCL
PRKCL
nPKC-eta
Species Homo sapiens
 Database cross references - PRKCH
ExoCarta ExoCarta_5583
Entrez Gene 5583
HGNC 9403
MIM 605437
UniProt P24723  
 PRKCH identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Urine 19056867    
 Gene ontology annotations for PRKCH
Molecular Function
    metal ion binding GO:0046872 IEA
    enzyme binding GO:0019899 IPI
    ATP binding GO:0005524 IEA
    Ral GTPase binding GO:0017160 IEA
    protein kinase C activity GO:0004697 TAS
    calcium-independent protein kinase C activity GO:0004699 IEA
Biological Process
    platelet activation GO:0030168 TAS
    positive regulation of keratinocyte differentiation GO:0045618 ISS
    regulation of bicellular tight junction assembly GO:2000810 IMP
    blood coagulation GO:0007596 TAS
    positive regulation of glial cell proliferation GO:0060252 IMP
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IMP
    protein phosphorylation GO:0006468 IMP
    positive regulation of B cell receptor signaling pathway GO:0050861 IMP
    signal transduction GO:0007165 TAS
    protein kinase C signaling GO:0070528 IEA
    positive regulation of macrophage derived foam cell differentiation GO:0010744 ISS
    negative regulation of glial cell apoptotic process GO:0034351 IMP
Subcellular Localization
    cytoplasm GO:0005737 IDA
    extracellular exosome GO:0070062 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    cell-cell junction GO:0005911 IEA
 Experiment description of studies that identified PRKCH in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for PRKCH
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDK2 1017
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
View the network image/svg+xml
 Pathways in which PRKCH is involved
PathwayEvidenceSource
Effects of PIP2 hydrolysis TAS Reactome
G alpha (z) signalling events TAS Reactome





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