Gene description for ITGB2
Gene name integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
Gene symbol ITGB2
Other names/aliases CD18
LAD
LCAMB
LFA-1
MAC-1
MF17
MFI7
Species Homo sapiens
 Database cross references - ITGB2
ExoCarta ExoCarta_3689
Vesiclepedia VP_3689
Entrez Gene 3689
HGNC 6155
MIM 600065
UniProt P05107  
 ITGB2 identified in exosomes derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
T cells 11907077    
Thymus 23844026    
 Gene ontology annotations for ITGB2
Molecular Function
    protein kinase binding GO:0019901 IPI
    cell adhesion molecule binding GO:0050839 IMP
    glycoprotein binding GO:0001948 IPI
    metal ion binding GO:0046872 IEA
    ICAM-3 receptor activity GO:0030369 IMP
    protein heterodimerization activity GO:0046982 IEA
    protein binding GO:0005515 IPI
    protein complex binding GO:0032403 IEA
Biological Process
    integrin-mediated signaling pathway GO:0007229 NAS
    positive regulation of angiogenesis GO:0045766 IEA
    innate immune response GO:0045087 TAS
    apoptotic process GO:0006915 NAS
    regulation of cell shape GO:0008360 NAS
    leukocyte migration GO:0050900 TAS
    cell-cell signaling GO:0007267 NAS
    neutrophil chemotaxis GO:0030593 IDA
    regulation of immune response GO:0050776 TAS
    inflammatory response GO:0006954 NAS
    cell adhesion GO:0007155 TAS
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IEA
    activated T cell proliferation GO:0050798 IEA
    blood coagulation GO:0007596 TAS
    regulation of peptidyl-tyrosine phosphorylation GO:0050730 IDA
    aging GO:0007568 IEA
    toll-like receptor signaling pathway GO:0002224 TAS
    cell-matrix adhesion GO:0007160 IMP
    extracellular matrix organization GO:0030198 TAS
    heterotypic cell-cell adhesion GO:0034113 IMP
    endodermal cell differentiation GO:0035987 IEP
    natural killer cell activation GO:0030101 IEA
    toll-like receptor 4 signaling pathway GO:0034142 TAS
    leukocyte migration involved in inflammatory response GO:0002523 IEA
    leukocyte cell-cell adhesion GO:0007159 IDA
    receptor clustering GO:0043113 IMP
    cellular extravasation GO:0045123 IEA
    positive regulation of nitric oxide biosynthetic process GO:0045429 IEA
    endothelial cell migration GO:0043542 IEA
Subcellular Localization
    membrane GO:0016020 IDA
    plasma membrane GO:0005886 TAS
    receptor complex GO:0043235 IDA
    extracellular vesicle GO:1903561 IDA
    cell surface GO:0009986 IDA
    extracellular exosome GO:0070062 IDA
    integrin alphaL-beta2 complex GO:0034687 IDA
    integrin complex GO:0008305 NAS
    membrane raft GO:0045121 IEA
 Experiment description of studies that identified ITGB2 in exosomes
1
Experiment ID 79
MISEV standards
✘ EV Biophysical techniques
✔
CD81|MHCII
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
MISEV standards
✘ EV Biophysical techniques
✔
CD81|MHCII
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
MISEV standards
✘ EV Biophysical techniques
✔
CD81|MHCII
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 231
MISEV standards
✘ EV Biophysical techniques
✔
Alix|CD63|CD9
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 232
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 233
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 18
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 11907077    
Organism Homo sapiens
Experiment description TCR activation of human T cells induces the production of exosomes bearing the TCR/CD3/zeta complex.
Authors Blanchard N, Lankar D, Faure F, Regnault A, Dumont C, Raposo G, Hivroz C
Journal name JIMMU
Publication year 2002
Sample T cells
Sample name Jurkat
T cell blasts
Isolation/purification methods Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
12
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ITGB2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD14 929
Affinity Capture-MS Homo sapiens
2 VNN2  
Invitro Homo sapiens
3 ITGAM 3684
Invivo Homo sapiens
4 P2rx7  
Affinity Capture-MS Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
5 ICAM2 3384
Affinity Capture-MS Homo sapiens
6 ICAM1 3383
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
7 PLAUR 5329
Affinity Capture-Western Homo sapiens
8 ITGAL 3683
Affinity Capture-Western Homo sapiens
9 CYTH1 9267
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
10 PTK2 5747
Affinity Capture-MS Homo sapiens
11 ITGAD  
Affinity Capture-Western Homo sapiens
12 ITGAX 3687
Invivo Homo sapiens
13 CD226 10666
Invivo Homo sapiens
14 ESM1 11082
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
15 HP 3240
Invivo Homo sapiens
16 PTK2B 2185
Affinity Capture-Western Homo sapiens
17 KNG1 3827
Invivo Homo sapiens
Invitro Homo sapiens
18 ICAM3 3385
Invivo Homo sapiens
Invitro Homo sapiens
19 COPS3 8533
Affinity Capture-MS Homo sapiens
20 GNB2L1 10399
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
21 ILK 3611
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
22 SYK 6850
Affinity Capture-Western Homo sapiens
23 ICAM5 7087
Invitro Homo sapiens
24 FHL2 2274
Invitro Homo sapiens
Two-hybrid Homo sapiens
25 CD82 3732
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
26 ICAM4  
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
27 FUT4  
Invivo Homo sapiens
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