Gene description for PRKCD
Gene name protein kinase C, delta
Gene symbol PRKCD
Other names/aliases ALPS3
CVID9
MAY1
PKCD
nPKC-delta
Species Homo sapiens
 Database cross references - PRKCD
ExoCarta ExoCarta_5580
Vesiclepedia VP_5580
Entrez Gene 5580
HGNC 9399
MIM 176977
UniProt Q05655  
 PRKCD identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Urine 19056867    
 Gene ontology annotations for PRKCD
Molecular Function
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    protein serine/threonine kinase activity GO:0004674 IDA
    ATP binding GO:0005524 IEA
    enzyme activator activity GO:0008047 IDA
    non-membrane spanning protein tyrosine kinase activity GO:0004715 IEA
    insulin receptor substrate binding GO:0043560 ISS
    protein kinase C activity GO:0004697 ISS
    enzyme binding GO:0019899 IPI
    calcium-independent protein kinase C activity GO:0004699 TAS
Biological Process
    programmed cell death GO:0012501 TAS
    innate immune response GO:0045087 TAS
    peptidyl-tyrosine phosphorylation GO:0018108 IEA
    positive regulation of ceramide biosynthetic process GO:2000304 IMP
    negative regulation of platelet aggregation GO:0090331 ISS
    regulation of actin cytoskeleton organization GO:0032956 IMP
    negative regulation of protein binding GO:0032091 TAS
    positive regulation of apoptotic signaling pathway GO:2001235 IEA
    negative regulation of peptidyl-tyrosine phosphorylation GO:0050732 ISS
    negative regulation of MAP kinase activity GO:0043407 IMP
    negative regulation of inflammatory response GO:0050728 IC
    interferon-gamma-mediated signaling pathway GO:0060333 TAS
    positive regulation of sphingomyelin catabolic process GO:2000755 IMP
    cellular component disassembly involved in execution phase of apoptosis GO:0006921 TAS
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
    gene expression GO:0010467 TAS
    cell cycle GO:0007049 IEA
    peptidyl-threonine phosphorylation GO:0018107 IDA
    stimulatory C-type lectin receptor signaling pathway GO:0002223 TAS
    positive regulation of protein dephosphorylation GO:0035307 IMP
    protein phosphorylation GO:0006468 IDA
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    immunoglobulin mediated immune response GO:0016064 IEA
    negative regulation of insulin receptor signaling pathway GO:0046627 TAS
    regulation of receptor activity GO:0010469 TAS
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    negative regulation of actin filament polymerization GO:0030837 ISS
    positive regulation of phospholipid scramblase activity GO:1900163 IMP
    negative regulation of glial cell apoptotic process GO:0034351 IMP
    Fc-gamma receptor signaling pathway involved in phagocytosis GO:0038096 TAS
    neutrophil activation GO:0042119 IDA
    signal transduction GO:0007165 TAS
    apoptotic process GO:0006915 TAS
    termination of signal transduction GO:0023021 IMP
    positive regulation of response to DNA damage stimulus GO:2001022 IMP
    interleukin-10 production GO:0032613 IEA
    cellular senescence GO:0090398 IMP
    activation of phospholipase C activity GO:0007202 TAS
    B cell proliferation GO:0042100 IEA
    defense response to bacterium GO:0042742 ISS
    blood coagulation GO:0007596 TAS
    platelet activation GO:0030168 TAS
    positive regulation of superoxide anion generation GO:0032930 IMP
    cytokine-mediated signaling pathway GO:0019221 TAS
    vascular endothelial growth factor receptor signaling pathway GO:0048010 TAS
    protein stabilization GO:0050821 NAS
    positive regulation of glucosylceramide catabolic process GO:2000753 IMP
    negative regulation of filopodium assembly GO:0051490 ISS
    intrinsic apoptotic signaling pathway in response to oxidative stress GO:0008631 TAS
    interleukin-12 production GO:0032615 IEA
Subcellular Localization
    nucleus GO:0005634 IDA
    nuclear matrix GO:0016363 IEA
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 TAS
    extracellular exosome GO:0070062 IDA
    plasma membrane GO:0005886 TAS
    perinuclear region of cytoplasm GO:0048471 IEA
    cell-cell junction GO:0005911 IEA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 IEA
    endoplasmic reticulum GO:0005783 IDA
 Experiment description of studies that identified PRKCD in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 231
MISEV standards
✘ EV Biophysical techniques
✔
Alix|CD63|CD9
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 232
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 233
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 63
MISEV standards
✘ EV Biophysical techniques
✔
AQP2
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for PRKCD
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GNA12 2768
Biochemical Activity Homo sapiens
2 RASGRP3  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
3 SHC1 6464
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 PTPN6 5777
Invivo Homo sapiens
Invitro Homo sapiens
5 LMNB1 4001
Invivo Homo sapiens
6 PPP2R5C 5527
Invitro Homo sapiens
Invivo Homo sapiens
7 INSR 3643
Affinity Capture-Western Homo sapiens
8 PDPK1 5170
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
9 RIPK4  
Affinity Capture-Western Homo sapiens
10 PLSCR3 57048
Biochemical Activity Homo sapiens
11 IL6ST 3572
Invivo Homo sapiens
12 GABRA1  
Invivo Homo sapiens
13 AFAP1 60312
Invitro Homo sapiens
14 STAT1 6772
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
15 MTOR 2475
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
16 ADAM9 8754
Affinity Capture-Western Homo sapiens
17 PTK2B 2185
Affinity Capture-Western Homo sapiens
18 PDP2  
Affinity Capture-Western Homo sapiens
19 PIK3CA 5290
Invitro Homo sapiens
20 C1QBP 708
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
21 MUC1 4582
Invitro Homo sapiens
Invivo Homo sapiens
22 GNA13 10672
Biochemical Activity Homo sapiens
23 YWHAG 7532
Affinity Capture-Western Homo sapiens
24 INPP5D 3635
Affinity Capture-Western Homo sapiens
25 TIAM1  
Invitro Homo sapiens
26 PDP1 54704
Affinity Capture-Western Homo sapiens
27 IRS1 3667
Invivo Homo sapiens
28 PLD2 5338
Invivo Homo sapiens
Invitro Homo sapiens
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