Gene description for EBI3
Gene name Epstein-Barr virus induced 3
Gene symbol EBI3
Other names/aliases IL-27B
IL27B
Species Homo sapiens
 Database cross references - EBI3
ExoCarta ExoCarta_10148
Vesiclepedia VP_10148
Entrez Gene 10148
HGNC 3129
MIM 605816
UniProt Q14213  
 EBI3 identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
 Gene ontology annotations for EBI3
Molecular Function
    cytokine receptor activity GO:0004896 IPI
    cytokine activity GO:0005125 IEA
    protein binding GO:0005515 IPI
    interleukin-27 receptor binding GO:0045523 IEA
Biological Process
    humoral immune response GO:0006959 TAS
    positive regulation of type II interferon production GO:0032729 TAS
    T-helper 1 type immune response GO:0042088 TAS
    T cell proliferation GO:0042098 IEA
    positive regulation of alpha-beta T cell proliferation GO:0046641 TAS
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IEA
    endoplasmic reticulum lumen GO:0005788 TAS
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified EBI3 in sEVs
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for EBI3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 IL27RA 9466
Affinity Capture-Western Homo sapiens
2 HECTD3 79654
Affinity Capture-MS Homo sapiens
3 MAEA 10296
Affinity Capture-MS Homo sapiens
4 ZMYND19  
Affinity Capture-MS Homo sapiens
5 ZZEF1 23140
Affinity Capture-MS Homo sapiens
6 MDFI  
Two-hybrid Homo sapiens
7 CANX 821
Affinity Capture-Western Homo sapiens
8 PRKCD 5580
Affinity Capture-MS Homo sapiens
9 SMAD3 4088
Two-hybrid Homo sapiens
10 ARMC8 25852
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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