Gene description for CASP8
Gene name caspase 8, apoptosis-related cysteine peptidase
Gene symbol CASP8
Other names/aliases ALPS2B
CAP4
Casp-8
FLICE
MACH
MCH5
Species Homo sapiens
 Database cross references - CASP8
ExoCarta ExoCarta_841
Vesiclepedia VP_841
Entrez Gene 841
HGNC 1509
MIM 601763
UniProt Q14790  
 CASP8 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for CASP8
Molecular Function
    protein heterodimerization activity GO:0046982 IEA
    protein complex binding GO:0032403 IEA
    scaffold protein binding GO:0097110 IPI
    death receptor binding GO:0005123 IEA
    protein binding GO:0005515 IPI
    cysteine-type endopeptidase activity GO:0004197 TAS
    peptidase activity GO:0008233 IMP
    tumor necrosis factor receptor binding GO:0005164 IEA
    death effector domain binding GO:0035877 IPI
    ubiquitin protein ligase binding GO:0031625 IPI
    cysteine-type peptidase activity GO:0008234 TAS
    cysteine-type endopeptidase activity involved in apoptotic signaling pathway GO:0097199 IMP
    cysteine-type endopeptidase activity involved in apoptotic process GO:0097153 IMP
Biological Process
    death-inducing signaling complex assembly GO:0071550 TAS
    toll-like receptor 3 signaling pathway GO:0034138 TAS
    cellular response to mechanical stimulus GO:0071260 IEP
    cellular response to organic cyclic compound GO:0071407 IEA
    negative regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043154 TAS
    response to tumor necrosis factor GO:0034612 IMP
    response to estradiol GO:0032355 IEA
    toll-like receptor signaling pathway GO:0002224 TAS
    natural killer cell activation GO:0030101 TAS
    execution phase of apoptosis GO:0097194 IMP
    proteolysis involved in cellular protein catabolic process GO:0051603 IMP
    nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway GO:0035872 TAS
    regulation of necrotic cell death GO:0010939 TAS
    positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway GO:1900740 TAS
    viral process GO:0016032 IEA
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 IMP
    T cell activation GO:0042110 TAS
    positive regulation of macrophage differentiation GO:0045651 IMP
    hepatocyte apoptotic process GO:0097284 IEA
    neural tube formation GO:0001841 IEA
    response to lipopolysaccharide GO:0032496 IEA
    regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902041 TAS
    negative regulation of necroptotic process GO:0060546 IEA
    apoptotic process GO:0006915 TAS
    necroptotic process GO:0070266 TAS
    cell surface receptor signaling pathway GO:0007166 TAS
    angiogenesis GO:0001525 IEA
    programmed necrotic cell death GO:0097300 TAS
    MyD88-independent toll-like receptor signaling pathway GO:0002756 TAS
    intrinsic apoptotic signaling pathway GO:0097193 TAS
    response to antibiotic GO:0046677 IEA
    positive regulation of extrinsic apoptotic signaling pathway GO:2001238 IEA
    response to ethanol GO:0045471 IEA
    protein heterooligomerization GO:0051291 IEA
    proteolysis GO:0006508 IDA
    regulation of thymocyte apoptotic process GO:0070243 IEA
    TRIF-dependent toll-like receptor signaling pathway GO:0035666 TAS
    activation of cysteine-type endopeptidase activity GO:0097202 IDA
    cellular component disassembly involved in execution phase of apoptosis GO:0006921 TAS
    activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway GO:0097296 TAS
    positive regulation of proteolysis GO:0045862 IDA
    response to cobalt ion GO:0032025 IEA
    negative regulation of I-kappaB kinase/NF-kappaB signaling GO:0043124 IMP
    tumor necrosis factor-mediated signaling pathway GO:0033209 TAS
    activation of cysteine-type endopeptidase activity involved in apoptotic process GO:0006919 TAS
    heart development GO:0007507 IEA
    programmed cell death GO:0012501 TAS
    TRAIL-activated apoptotic signaling pathway GO:0036462 IDA
    syncytiotrophoblast cell differentiation involved in labyrinthine layer development GO:0060715 TAS
    B cell activation GO:0042113 TAS
    macrophage differentiation GO:0030225 TAS
    toll-like receptor 4 signaling pathway GO:0034142 TAS
    extrinsic apoptotic signaling pathway GO:0097191 TAS
    stimulatory C-type lectin receptor signaling pathway GO:0002223 TAS
    response to cold GO:0009409 IEA
    nucleotide-binding oligomerization domain containing signaling pathway GO:0070423 TAS
    innate immune response GO:0045087 TAS
    apoptotic signaling pathway GO:0097190 TAS
Subcellular Localization
    microtubule organizing center GO:0005815 IDA
    mitochondrion GO:0005739 IDA
    cytoskeleton GO:0005856 TAS
    mitochondrial outer membrane GO:0005741 TAS
    nucleoplasm GO:0005654 IDA
    ripoptosome GO:0097342 IDA
    death-inducing signaling complex GO:0031264 IDA
    membrane raft GO:0045121 IEA
    CD95 death-inducing signaling complex GO:0031265 IDA
    neuron projection GO:0043005 IEA
    cytoplasm GO:0005737 IDA
    cell body GO:0044297 IEA
    Noc1p-Noc2p complex GO:0030690 IEA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified CASP8 in exosomes
1
Experiment ID 489
ISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
ISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
ISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
ISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
ISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for CASP8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 APAF1 317
Affinity Capture-Western Homo sapiens
2 BIRC2  
Affinity Capture-Western Homo sapiens
3 CASP8 841
Invitro Homo sapiens
Two-hybrid Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
4 MAP3K14  
Invivo Homo sapiens
5 TFCP2 7024
Two-hybrid Homo sapiens
6 CHD3 1107
Two-hybrid Homo sapiens
7 TRAF2 7186
Affinity Capture-Western Homo sapiens
8 TRAF1 7185
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
9 CFLAR  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
10 FAS 355
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
11 RALBP1 10928
Affinity Capture-Western Homo sapiens
12 BID  
Co-purification Homo sapiens
13 CTNNB1 1499
Invivo Homo sapiens
14 EZR 7430
Co-purification Homo sapiens
15 FASLG 356
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
16 BCAP31 10134
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
17 IKBKB 3551
Invivo Homo sapiens
18 TRADD 8717
Affinity Capture-Western Homo sapiens
19 CDH3 1001
Invivo Homo sapiens
Invitro Homo sapiens
20 MAP4K4 9448
Invivo Homo sapiens
21 PICALM 8301
Invitro Homo sapiens
22 RIPK1 8737
Invivo Homo sapiens
23 CASP14 23581
Invivo Homo sapiens
24 APP 351
Invitro Homo sapiens
25 FADD 8772
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
26 ARHGDIA 396
Co-purification Homo sapiens
27 TNFRSF10B 8795
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
28 NR1H4  
Reconstituted Complex Homo sapiens
29 CDH1 999
Invivo Homo sapiens
30 BCL2  
Invivo Homo sapiens
31 DEDD2  
Invitro Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
32 NOD1 10392
Affinity Capture-Western Homo sapiens
33 CASP6 839
Invivo Homo sapiens
Invitro Homo sapiens
34 CASP10  
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
35 HIP1 3092
Invivo Homo sapiens
36 CRADD  
Invivo Homo sapiens
37 CASP8AP2  
Invivo Homo sapiens
Invivo Homo sapiens
38 MSN 4478
Co-purification Homo sapiens
39 VIM 7431
Two-hybrid Homo sapiens
40 RHOA 387
Co-purification Homo sapiens
41 MAPK8 5599
Co-purification Homo sapiens
42 NOL3 8996
Invivo Homo sapiens
Invitro Homo sapiens
43 CHUK 1147
Invivo Homo sapiens
44 PEA15 8682
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
45 DEDD  
Invitro Homo sapiens
46 IFT57 55081
Invivo Homo sapiens
47 TNFRSF10A 8797
Affinity Capture-Western Homo sapiens
48 TNFRSF1A 7132
Co-purification Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here