Gene description for CASP9
Gene name caspase 9, apoptosis-related cysteine peptidase
Gene symbol CASP9
Other names/aliases APAF-3
APAF3
ICE-LAP6
MCH6
PPP1R56
Species Homo sapiens
 Database cross references - CASP9
ExoCarta ExoCarta_842
Vesiclepedia VP_842
Entrez Gene 842
HGNC 1511
MIM 602234
UniProt P55211  
 CASP9 identified in exosomes derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for CASP9
Molecular Function
    cysteine-type endopeptidase activity GO:0004197 TAS
    protein binding GO:0005515 IPI
    cysteine-type endopeptidase activity involved in apoptotic signaling pathway GO:0097199 IBA
    peptidase activity GO:0008233 IDA
    protein kinase binding GO:0019901 IDA
    SH3 domain binding GO:0017124 IDA
    enzyme activator activity GO:0008047 TAS
Biological Process
    intrinsic apoptotic signaling pathway in response to DNA damage GO:0008630 IMP
    intrinsic apoptotic signaling pathway GO:0097193 TAS
    response to cobalt ion GO:0032025 IEA
    programmed cell death GO:0012501 TAS
    response to estradiol GO:0032355 IEA
    cellular response to DNA damage stimulus GO:0006974 IDA
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    extrinsic apoptotic signaling pathway GO:0097191 TAS
    aging GO:0007568 IEA
    signal transduction in response to DNA damage GO:0042770 IDA
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    cellular response to UV GO:0034644 IDA
    glial cell apoptotic process GO:0034349 IEA
    positive regulation of neuron apoptotic process GO:0043525 IEA
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
    response to antibiotic GO:0046677 IEA
    phosphatidylinositol-mediated signaling GO:0048015 TAS
    response to lipopolysaccharide GO:0032496 IEA
    cellular response to dexamethasone stimulus GO:0071549 IEA
    extrinsic apoptotic signaling pathway in absence of ligand GO:0097192 TAS
    regulation of response to DNA damage stimulus GO:2001020 IMP
    innate immune response GO:0045087 TAS
    platelet formation GO:0030220 TAS
    apoptotic process GO:0006915 TAS
    activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c GO:0008635 TAS
Subcellular Localization
    mitochondrion GO:0005739 IEA
    cytosol GO:0005829 TAS
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IBA
    apoptosome GO:0043293 IDA
 Experiment description of studies that identified CASP9 in exosomes
1
Experiment ID 80
MISEV standards
✘ EV Biophysical techniques
✔
CD81|MHCII
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 81
MISEV standards
✘ EV Biophysical techniques
✔
CD81|MHCII
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for CASP9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MAPK1 5594
Biochemical Activity Homo sapiens
2 DCC  
Affinity Capture-Western Homo sapiens
3 NOD1 10392
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
4 GZMB  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
5 BIRC2  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
6 CYCS 54205
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
7 BIRC7  
Affinity Capture-Western Homo sapiens
8 XIAP  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
Reconstituted Complex Homo sapiens
9 CASP9 842
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 BCL2L1 598
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
11 APAF1 317
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
12 BIRC1  
Far Western Homo sapiens
13 NALP1  
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
14 BIRC8  
Reconstituted Complex Homo sapiens
15 BIRC5  
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
16 CASP10  
Invitro Homo sapiens
17 BIRC3 330
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
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