Gene description for TRADD
Gene name TNFRSF1A-associated via death domain
Gene symbol TRADD
Other names/aliases Hs.89862
Species Homo sapiens
 Database cross references - TRADD
ExoCarta ExoCarta_8717
Vesiclepedia VP_8717
Entrez Gene 8717
HGNC 12030
MIM 603500
UniProt Q15628  
 TRADD identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for TRADD
Molecular Function
    kinase binding GO:0019900 IPI
    tumor necrosis factor receptor binding GO:0005164 IEA
    protein complex binding GO:0032403 IEA
    identical protein binding GO:0042802 IPI
    signal transducer activity GO:0004871 IEA
    death domain binding GO:0070513 IPI
    protein binding GO:0005515 IPI
    binding, bridging GO:0060090 IPI
Biological Process
    extrinsic apoptotic signaling pathway via death domain receptors GO:0008625 TAS
    apoptotic process GO:0006915 TAS
    tumor necrosis factor-mediated signaling pathway GO:0033209 TAS
    signal transduction GO:0007165 TAS
    necroptotic process GO:0070266 TAS
    negative regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043154 TAS
    death-inducing signaling complex assembly GO:0071550 TAS
    positive regulation of apoptotic process GO:0043065 TAS
    regulation of necrotic cell death GO:0010939 TAS
    activation of cysteine-type endopeptidase activity involved in apoptotic process GO:0006919 TAS
    programmed cell death GO:0012501 TAS
    cell surface receptor signaling pathway GO:0007166 TAS
    programmed necrotic cell death GO:0097300 TAS
    positive regulation of NF-kappaB transcription factor activity GO:0051092 TAS
    extrinsic apoptotic signaling pathway GO:0097191 TAS
    activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway GO:0097296 TAS
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 IEP
    protein heterooligomerization GO:0051291 IEA
    positive regulation of hair follicle development GO:0051798 IEA
    regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902041 TAS
Subcellular Localization
    receptor complex GO:0043235 IDA
    nucleus GO:0005634 IEA
    cytoskeleton GO:0005856 IEA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    membrane raft GO:0045121 IEA
    death-inducing signaling complex GO:0031264 TAS
 Experiment description of studies that identified TRADD in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TRADD
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KRT18 3875
Invivo Homo sapiens
Invitro Homo sapiens
2 TNF  
Affinity Capture-Western Homo sapiens
3 BIRC2  
Invivo Homo sapiens
4 TNFRSF1A 7132
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
5 FADD 8772
Invivo Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
6 TRAF2 7186
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
7 TNFRSF25  
Two-hybrid Homo sapiens
8 TES 26136
Invivo Homo sapiens
Two-hybrid Homo sapiens
9 FAS 355
Invivo Homo sapiens
10 CASP8 841
Affinity Capture-Western Homo sapiens
11 BCL10  
Affinity Capture-Western Homo sapiens
12 RIPK1 8737
Invivo Homo sapiens
Invitro Homo sapiens
13 TRAF3 7187
Invivo Homo sapiens
Two-hybrid Homo sapiens
14 TNFRSF10B 8795
Affinity Capture-Western Homo sapiens
15 STAT1 6772
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
16 TNFRSF21 27242
Affinity Capture-Western Homo sapiens
17 NGFR 4804
Affinity Capture-Western Homo sapiens
18 KRT14 3861
Invitro Homo sapiens
19 HIPK2 28996
Invitro Homo sapiens
Two-hybrid Homo sapiens
20 CAV1 857
Affinity Capture-Western Homo sapiens
21 TRPC4AP  
Affinity Capture-Western Homo sapiens
22 TNFRSF10A 8797
Affinity Capture-Western Homo sapiens
23 TRAF1 7185
Invivo Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here