Gene description for SORBS1
Gene name sorbin and SH3 domain containing 1
Gene symbol SORBS1
Other names/aliases CAP
FLAF2
R85FL
SH3D5
SH3P12
SORB1
Species Homo sapiens
 Database cross references - SORBS1
ExoCarta ExoCarta_10580
Vesiclepedia VP_10580
Entrez Gene 10580
HGNC 14565
MIM 605264
UniProt Q9BX66  
 SORBS1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
Neuroblastoma cells 25944692    
 Gene ontology annotations for SORBS1
Molecular Function
    actin binding GO:0003779 TAS
    insulin receptor binding GO:0005158 IDA
    protein binding GO:0005515 IPI
    cytoskeletal protein binding GO:0008092 TAS
    signaling receptor complex adaptor activity GO:0030159 IDA
    signaling receptor complex adaptor activity GO:0030159 ISS
Biological Process
    cell-matrix adhesion GO:0007160 TAS
    insulin receptor signaling pathway GO:0008286 ISS
    cell-substrate adhesion GO:0031589 IBA
    cellular response to insulin stimulus GO:0032869 ISS
    stress fiber assembly GO:0043149 ISS
    positive regulation of glycogen biosynthetic process GO:0045725 ISS
    positive regulation of D-glucose import GO:0046326 ISS
    positive regulation of insulin receptor signaling pathway GO:0046628 ISS
    positive regulation of lipid biosynthetic process GO:0046889 ISS
    focal adhesion assembly GO:0048041 ISS
    positive regulation of protein localization to plasma membrane GO:1903078 ISS
Subcellular Localization
    stress fiber GO:0001725 ISS
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IBA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 ISS
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    adherens junction GO:0005912 ISS
    zonula adherens GO:0005915 TAS
    focal adhesion GO:0005925 IDA
    nuclear matrix GO:0016363 IEA
    flotillin complex GO:0016600 ISS
    cell-substrate junction GO:0030055 IBA
    cell-substrate junction GO:0030055 ISS
    membrane raft GO:0045121 ISS
 Experiment description of studies that identified SORBS1 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
6
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for SORBS1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
2 Tor1aip1  
Affinity Capture-MS Mus musculus
3 Sidt2  
Affinity Capture-MS Mus musculus
4 YWHAG 7532
Affinity Capture-MS Homo sapiens
5 FBXL6  
Proximity Label-MS Homo sapiens
6 PXN 5829
Proximity Label-MS Homo sapiens
7 KIF14 9928
Affinity Capture-MS Homo sapiens
8 PRNP 5621
Affinity Capture-MS Homo sapiens
9 EIF6 3692
Affinity Capture-MS Homo sapiens
10 YWHAB 7529
Affinity Capture-MS Homo sapiens
11 INPPL1 3636
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
12 PARD3 56288
Proximity Label-MS Homo sapiens
13 INSM1  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
14 TGS1  
Affinity Capture-MS Homo sapiens
15 EZR 7430
Proximity Label-MS Homo sapiens
16 SOS1 6654
Affinity Capture-Western Homo sapiens
17 ACTC1 70
Proximity Label-MS Homo sapiens
18 CASP8 841
Affinity Capture-Western Homo sapiens
19 PRPH 5630
Proximity Label-MS Homo sapiens
20 RIN3  
Affinity Capture-MS Homo sapiens
21 ABL1 25
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
22 INSR 3643
Affinity Capture-Western Homo sapiens
23 RPL4 6124
Two-hybrid Homo sapiens
24 SLC10A3 8273
Two-hybrid Homo sapiens
25 CDK6 1021
Biochemical Activity Homo sapiens
26 MAP4K3 8491
Two-hybrid Homo sapiens
27 MSX2  
Affinity Capture-MS Homo sapiens
28 CBL 867
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
29 SH2B2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
30 CDH1 999
Proximity Label-MS Homo sapiens
31 VCL 7414
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
32 SEMA6A  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
33 DYNC1LI1 51143
Proximity Label-MS Homo sapiens
34 CDK4 1019
Affinity Capture-MS Homo sapiens
35 NTRK1 4914
Affinity Capture-Western Homo sapiens
36 FRS2 10818
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
37 PILRA 29992
Two-hybrid Homo sapiens
38 ACTR3 10096
Proximity Label-MS Homo sapiens
39 CXADR 1525
Proximity Label-MS Homo sapiens
40 CTNNB1 1499
Proximity Label-MS Homo sapiens
41 TENM1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
42 BICD2 23299
Proximity Label-MS Homo sapiens
43 BICD1 636
Proximity Label-MS Homo sapiens
44 ACTB 60
Proximity Label-MS Homo sapiens
45 SRC 6714
Biochemical Activity Homo sapiens
46 DYNC1LI2 1783
Proximity Label-MS Homo sapiens
47 KHDRBS1 10657
Protein-peptide Homo sapiens
48 CTNNA1 1495
Proximity Label-MS Homo sapiens
49 MLLT4 4301
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Proximity Label-MS Homo sapiens
50 AKT1 207
Biochemical Activity Homo sapiens
51 LATS2 26524
Proximity Label-MS Homo sapiens
52 FLOT1 10211
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
53 Zbtb2 381990
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which SORBS1 is involved
PathwayEvidenceSource
Muscle contraction TAS Reactome
Smooth Muscle Contraction TAS Reactome





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