Gene description for SORBS1
Gene name sorbin and SH3 domain containing 1
Gene symbol SORBS1
Other names/aliases CAP
FLAF2
R85FL
SH3D5
SH3P12
SORB1
Species Homo sapiens
 Database cross references - SORBS1
ExoCarta ExoCarta_10580
Vesiclepedia VP_10580
Entrez Gene 10580
HGNC 14565
MIM 605264
UniProt Q9BX66  
 SORBS1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
Neuroblastoma cells 25944692    
 Gene ontology annotations for SORBS1
Molecular Function
    actin binding GO:0003779 TAS
    protein binding GO:0005515 IPI
    SH3/SH2 adaptor activity GO:0005070 ISS
    protein kinase binding GO:0019901 IEA
    cytoskeletal protein binding GO:0008092 TAS
    insulin receptor binding GO:0005158 IDA
Biological Process
    stress fiber assembly GO:0043149 ISS
    positive regulation of lipid biosynthetic process GO:0046889 ISS
    glucose transport GO:0015758 ISS
    positive regulation of signal transduction GO:0009967 ISS
    cell-matrix adhesion GO:0007160 TAS
    positive regulation of glycogen biosynthetic process GO:0045725 ISS
    cellular response to insulin stimulus GO:0032869 ISS
    focal adhesion assembly GO:0048041 ISS
    insulin receptor signaling pathway GO:0008286 ISS
    positive regulation of establishment of protein localization to plasma membrane GO:0090004 ISS
    muscle contraction GO:0006936 TAS
    positive regulation of glucose import GO:0046326 ISS
Subcellular Localization
    centrosome GO:0005813 IDA
    nucleus GO:0005634 IDA
    stress fiber GO:0001725 ISS
    focal adhesion GO:0005925 IDA
    membrane raft GO:0045121 ISS
    cytoplasm GO:0005737 IDA
    insulin receptor complex GO:0005899 IDA
    nucleoplasm GO:0005654 IDA
    zonula adherens GO:0005915 TAS
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    cell-substrate adherens junction GO:0005924 ISS
    nuclear matrix GO:0016363 IEA
    cell-cell adherens junction GO:0005913 ISS
 Experiment description of studies that identified SORBS1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
6
Experiment ID 224
MISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101|CD63|CD81
EV Enriched markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for SORBS1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CBL 867
Affinity Capture-Western Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
2 INSM1  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
3 MLLT4 4301
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 ABL1 25
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
5 INSR 3643
Invivo Homo sapiens
Invitro Homo sapiens
6 FLOT1  
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
7 VCL 7414
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
8 PTK2B 2185
Affinity Capture-Western Homo sapiens
9 EIF6 3692
Affinity Capture-MS Homo sapiens
10 INPPL1 3636
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which SORBS1 is involved
PathwayEvidenceSource
Smooth Muscle Contraction TAS Reactome





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