Gene description for FRS2
Gene name fibroblast growth factor receptor substrate 2
Gene symbol FRS2
Other names/aliases FRS2A
FRS2alpha
SNT
SNT-1
SNT1
Species Homo sapiens
 Database cross references - FRS2
ExoCarta ExoCarta_10818
Vesiclepedia VP_10818
Entrez Gene 10818
HGNC 16971
MIM 607743
UniProt Q8WU20  
 FRS2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for FRS2
Molecular Function
    fibroblast growth factor receptor binding GO:0005104 IPI
    neurotrophin TRKA receptor binding GO:0005168 IPI
    insulin receptor binding GO:0005158 IEA
    protein binding GO:0005515 IPI
    transmembrane receptor protein tyrosine kinase adaptor activity GO:0005068 TAS
    phosphatase activator activity GO:0019211 TAS
Biological Process
    ventricular septum development GO:0003281 IEA
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    optic placode formation involved in camera-type eye formation GO:0046619 IEA
    regulation of epithelial cell proliferation GO:0050678 IEA
    prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis GO:0060527 IEA
    organ induction GO:0001759 IEA
    anterior/posterior axis specification, embryo GO:0008595 IEA
    regulation of apoptotic process GO:0042981 IEA
    insulin receptor signaling pathway GO:0008286 TAS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    phosphatidylinositol-mediated signaling GO:0048015 TAS
    G-protein coupled receptor signaling pathway GO:0007186 TAS
    activation of MAPK activity GO:0000187 IEA
    lens fiber cell development GO:0070307 IEA
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    regulation of ERK1 and ERK2 cascade GO:0070372 IEA
    forebrain development GO:0030900 IEA
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
    innate immune response GO:0045087 TAS
    activation of MAPKK activity GO:0000186 TAS
    transmembrane receptor protein tyrosine phosphatase signaling pathway GO:0007185 TAS
    gastrulation with mouth forming second GO:0001702 IEA
    neuroblast proliferation GO:0007405 IEA
Subcellular Localization
    endosome GO:0005768 TAS
    membrane GO:0016020 TAS
    plasma membrane GO:0005886 TAS
    integral component of plasma membrane GO:0005887 TAS
    cell-cell junction GO:0005911 IEA
 Experiment description of studies that identified FRS2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
EV Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for FRS2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CBL 867
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
2 PRKCI 5584
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
3 SOS1 6654
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
4 PTPN11 5781
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
5 NTRK2 4915
Reconstituted Complex Homo sapiens
6 FGFR1 2260
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
7 INSR 3643
Biochemical Activity Homo sapiens
8 GRB2 2885
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 NTRK1 4914
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
10 RET 5979
Invitro Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
11 CRK 1398
Reconstituted Complex Homo sapiens
12 NTRK3 4916
Reconstituted Complex Homo sapiens
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