Gene description for TNFSF10
Gene name tumor necrosis factor (ligand) superfamily, member 10
Gene symbol TNFSF10
Other names/aliases APO2L
Apo-2L
CD253
TL2
TRAIL
Species Homo sapiens
 Database cross references - TNFSF10
ExoCarta ExoCarta_8743
Vesiclepedia VP_8743
Entrez Gene 8743
HGNC 11925
MIM 603598
UniProt P50591  
 TNFSF10 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 15940614    
Saliva 19199708    
 Gene ontology annotations for TNFSF10
Molecular Function
    signaling receptor binding GO:0005102 TAS
    cytokine activity GO:0005125 IBA
    cytokine activity GO:0005125 IDA
    tumor necrosis factor receptor binding GO:0005164 IEA
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IDA
    identical protein binding GO:0042802 IDA
    TRAIL binding GO:0045569 IEA
Biological Process
    apoptotic process GO:0006915 TAS
    immune response GO:0006955 IEA
    signal transduction GO:0007165 TAS
    cell surface receptor signaling pathway GO:0007166 IBA
    cell-cell signaling GO:0007267 TAS
    male gonad development GO:0008584 IEA
    response to insulin GO:0032868 IEA
    TRAIL-activated apoptotic signaling pathway GO:0036462 IDA
    positive regulation of apoptotic process GO:0043065 IDA
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IBA
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IEP
    positive regulation of release of cytochrome c from mitochondria GO:0090200 IDA
    positive regulation of cysteine-type endopeptidase activity GO:2001056 IDA
    positive regulation of extrinsic apoptotic signaling pathway GO:2001238 IBA
    positive regulation of extrinsic apoptotic signaling pathway GO:2001238 IDA
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    plasma membrane GO:0005886 IEA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified TNFSF10 in exosomes
1
Experiment ID 22
MISEV standards
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
Immunoelectron Microscopy
PubMed ID 15940614    
Organism Homo sapiens
Experiment description Human colorectal cancer cells induce T-cell death through release of proapoptotic microvesicles: role in immune escape.
Authors "Huber V, Fais S, Iero M, Lugini L, Canese P, Squarcina P, Zaccheddu A, Colone M, Arancia G, Gentile M, Seregni E, Valenti R, Ballabio G, Belli F, Leo E, Parmiani G, Rivoltini L."
Journal name GASTRO
Publication year 2005
Sample Colorectal cancer cells
Sample name SW403
1869col
CRC28462
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Immunoelectron Microscopy
FACS
2
Experiment ID 66
MISEV standards
IEM
Biophysical techniques
TSG101|Alix|CD63|CD81
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for TNFSF10
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NOMO1 23420
Affinity Capture-MS Homo sapiens
2 TRAF2 7186
Affinity Capture-Western Homo sapiens
3 RIPK1 8737
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
4 ZDHHC17 23390
Two-hybrid Homo sapiens
5 C18orf8 29919
Affinity Capture-MS Homo sapiens
6 CCZ1 51622
Affinity Capture-MS Homo sapiens
7 SOCS3 9021
Affinity Capture-Western Homo sapiens
8 FADD 8772
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 CASP8 841
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
10 RBM42  
Affinity Capture-MS Homo sapiens
11 TNFRSF10B 8795
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
12 CFLAR  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
13 ACP5  
Two-hybrid Homo sapiens
14 PEA15 8682
Affinity Capture-Western Homo sapiens
15 CASP3 836
Biochemical Activity Homo sapiens
16 TNFSF10 8743
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
17 TNFAIP3 7128
Affinity Capture-Western Homo sapiens
18 TNFRSF10A 8797
Affinity Capture-Western Homo sapiens
19 RBM48  
Two-hybrid Homo sapiens
20 TNFRSF10D 8793
Reconstituted Complex Homo sapiens
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