Gene ontology annotations for IQGAP2
Experiment description of studies that identified IQGAP2 in exosomes
1
Experiment ID
20
MISEV standards
✔
EM
Biophysical techniques
✔
HSP90|CD63|CD81|LAMP1
Enriched markers
✔
GOLGA2|cytochrome c
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
17956143
Organism
Homo sapiens
Experiment description
Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors
"Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS"
Journal name
JPR
Publication year
2007
Sample
Colorectal cancer cells
Sample name
HT29
Isolation/purification methods
Differential centrifugation Sucrose density gradient Diafiltration
Flotation density
1.16 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LTQ] Western blotting
2
Experiment ID
488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Homo sapiens
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Mesenchymal stem cells
Sample name
UCMSC
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
3
Experiment ID
224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25944692
Organism
Homo sapiens
Experiment description
Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors
"Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name
Oncotarget
Publication year
2015
Sample
Neuroblastoma cells
Sample name
SH-SY5Y
Isolation/purification methods
Differential centrifugation Ultracentrifugation OptiPrep density gradient
Flotation density
1.10 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
4
Experiment ID
211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
IGROV1
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
5
Experiment ID
212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
OVCAR-3
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
6
Experiment ID
231
MISEV standards
✘
Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
"Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Western blotting Mass spectrometry
7
Experiment ID
232
MISEV standards
✘
Biophysical techniques
✘
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
"Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
8
Experiment ID
233
MISEV standards
✘
Biophysical techniques
✘
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
"Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 3
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
9
Experiment ID
834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35333565
Organism
Homo sapiens
Experiment description
LAMP2A regulates the loading of proteins into exosomes
Authors
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name
Sci Adv
Publication year
2022
Sample
Retinal pigment epithelial cells
Sample name
ARPE-19
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
10
Experiment ID
835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35333565
Organism
Homo sapiens
Experiment description
LAMP2A regulates the loading of proteins into exosomes
Authors
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name
Sci Adv
Publication year
2022
Sample
Retinal pigment epithelial cells
Sample name
ARPE-19
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
11
Experiment ID
217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23844026
Organism
Homo sapiens
Experiment description
Characterization of human thymic exosomes.
Authors
"Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name
PLoS One
Publication year
2013
Sample
Thymus
Sample name
Normal-Thymus
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
12
Experiment ID
63
MISEV standards
✘
Biophysical techniques
✔
AQP2
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
19056867
Organism
Homo sapiens
Experiment description
Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors
"Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name
JASN
Publication year
2009
Sample
Urine
Sample name
Urine - Normal
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LTQ] Western blotting
Protein-protein interactions for IQGAP2
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
CD3EAP
Co-fractionation
Homo sapiens
2
C8orf76
Affinity Capture-MS
Homo sapiens
3
HECTD1
25831
Affinity Capture-MS
Homo sapiens
4
CALML3
810
Affinity Capture-MS
Homo sapiens
5
RELA
5970
Affinity Capture-MS
Homo sapiens
6
HNRNPCL2
440563
Affinity Capture-MS
Homo sapiens
7
GUSBP5
Affinity Capture-MS
Homo sapiens
8
RAB35
11021
Proximity Label-MS
Homo sapiens
9
PLEKHA4
57664
Affinity Capture-MS
Homo sapiens
10
LAMTOR1
55004
Proximity Label-MS
Homo sapiens
11
RHOG
391
Reconstituted Complex
Homo sapiens
12
UBAC1
10422
Affinity Capture-MS
Homo sapiens
13
OCLN
100506658
Proximity Label-MS
Homo sapiens
14
KIF14
9928
Affinity Capture-MS
Homo sapiens
15
IKBKB
3551
Co-fractionation
Homo sapiens
16
MYL6
4637
Affinity Capture-MS
Homo sapiens
17
MTIF2
4528
Co-fractionation
Homo sapiens
18
NFKB1
4790
Co-fractionation
Homo sapiens
19
TRIM52
Affinity Capture-MS
Homo sapiens
20
TSHR
7253
Affinity Capture-MS
Homo sapiens
21
FOXB1
Affinity Capture-MS
Homo sapiens
22
RABIF
Affinity Capture-MS
Homo sapiens
23
EBNA-LP
Affinity Capture-MS
24
MAGEA1
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
25
CCT8L2
Affinity Capture-MS
Homo sapiens
26
CUL3
8452
Affinity Capture-MS
Homo sapiens
27
C9orf78
51759
Affinity Capture-MS
Homo sapiens
28
SQSTM1
8878
Proximity Label-MS
Homo sapiens
29
CBWD2
Affinity Capture-MS
Homo sapiens
30
CALM1
801
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
31
ARF6
382
Proximity Label-MS
Homo sapiens
32
TXNDC12
51060
Co-fractionation
Homo sapiens
33
SUCLA2
8803
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
34
MAGEA9
Affinity Capture-MS
Homo sapiens
35
C11orf52
91894
Proximity Label-MS
Homo sapiens
36
LYN
4067
Proximity Label-MS
Homo sapiens
37
CDC42
998
Reconstituted Complex
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-Western
Homo sapiens
38
LRPPRC
10128
Co-fractionation
Homo sapiens
39
POLR1A
Co-fractionation
Homo sapiens
40
RPA3
6119
Proximity Label-MS
Homo sapiens
41
DTNBP1
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
42
ABTB2
25841
Affinity Capture-MS
Homo sapiens
43
MYC
Affinity Capture-MS
Homo sapiens
44
BTF3
689
Affinity Capture-MS
Homo sapiens
45
LMNA
4000
Cross-Linking-MS (XL-MS)
Homo sapiens
46
ARRDC3
57561
Affinity Capture-MS
Homo sapiens
47
RAC3
5881
Affinity Capture-MS
Homo sapiens
48
PARP1
142
Proximity Label-MS
Homo sapiens
49
PEX14
5195
Co-purification
Homo sapiens
50
S100B
6285
Affinity Capture-MS
Homo sapiens
51
ACTB
60
Proximity Label-MS
Homo sapiens
52
KRAS
3845
Proximity Label-MS
Homo sapiens
53
RHOB
388
Proximity Label-MS
Homo sapiens
54
GNAI2
2771
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
55
RAC1
5879
Reconstituted Complex
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-Western
Homo sapiens
56
PPP2R1A
5518
Affinity Capture-MS
Homo sapiens
57
MIER2
Affinity Capture-MS
Homo sapiens
58
ASIC2
Affinity Capture-MS
Homo sapiens
59
SDF4
51150
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
60
CBWD1
55871
Affinity Capture-MS
Homo sapiens
61
CAV1
857
Proximity Label-MS
Homo sapiens
62
EFCAB2
Affinity Capture-MS
Homo sapiens
63
CAMKK2
10645
Affinity Capture-MS
Homo sapiens
64
Ksr1
Affinity Capture-MS
Mus musculus
65
MYL12B
103910
Affinity Capture-MS
Homo sapiens
66
C9orf72
Affinity Capture-MS
Homo sapiens
View the network
image/svg+xml
Pathways in which IQGAP2 is involved