Gene description for IQGAP2
Gene name IQ motif containing GTPase activating protein 2
Gene symbol IQGAP2
Other names/aliases -
Species Homo sapiens
 Database cross references - IQGAP2
ExoCarta ExoCarta_10788
Vesiclepedia VP_10788
Entrez Gene 10788
HGNC 6111
MIM 605401
UniProt Q13576  
 IQGAP2 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 17956143    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for IQGAP2
Molecular Function
    actin binding GO:0003779 TAS
    GTPase inhibitor activity GO:0005095 TAS
    GTPase activator activity GO:0005096 IBA
    calmodulin binding GO:0005516 IBA
    calmodulin binding GO:0005516 IPI
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 IDA
    small GTPase binding GO:0031267 IDA
    actin filament binding GO:0051015 IBA
    actin filament binding GO:0051015 IDA
    Arp2/3 complex binding GO:0071933 IDA
Biological Process
    signal transduction GO:0007165 TAS
    regulation of actin cytoskeleton organization GO:0032956 IDA
    Arp2/3 complex-mediated actin nucleation GO:0034314 IDA
    thrombin-activated receptor signaling pathway GO:0070493 IDA
    mitotic actomyosin contractile ring assembly actin filament organization GO:1903479 IBA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    microtubule GO:0005874 IDA
    plasma membrane GO:0005886 TAS
    microvillus GO:0005902 IEA
    cell cortex GO:0005938 IBA
    cell surface GO:0009986 IDA
    actin cytoskeleton GO:0015629 TAS
    lamellipodium GO:0030027 IDA
    filopodium GO:0030175 IDA
    secretory granule membrane GO:0030667 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified IQGAP2 in exosomes
1
Experiment ID 20
MISEV standards
EM
Biophysical techniques
HSP90|CD63|CD81|LAMP1
Enriched markers
GOLGA2|cytochrome c
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors "Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS"
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
2
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
4
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 231
MISEV standards
Biophysical techniques
Alix|CD63|CD9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 232
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 233
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 63
MISEV standards
Biophysical techniques
AQP2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for IQGAP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD3EAP  
Co-fractionation Homo sapiens
2 C8orf76  
Affinity Capture-MS Homo sapiens
3 HECTD1 25831
Affinity Capture-MS Homo sapiens
4 CALML3 810
Affinity Capture-MS Homo sapiens
5 RELA 5970
Affinity Capture-MS Homo sapiens
6 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
7 GUSBP5  
Affinity Capture-MS Homo sapiens
8 RAB35 11021
Proximity Label-MS Homo sapiens
9 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
10 LAMTOR1 55004
Proximity Label-MS Homo sapiens
11 RHOG 391
Reconstituted Complex Homo sapiens
12 UBAC1 10422
Affinity Capture-MS Homo sapiens
13 OCLN 100506658
Proximity Label-MS Homo sapiens
14 KIF14 9928
Affinity Capture-MS Homo sapiens
15 IKBKB 3551
Co-fractionation Homo sapiens
16 MYL6 4637
Affinity Capture-MS Homo sapiens
17 MTIF2 4528
Co-fractionation Homo sapiens
18 NFKB1 4790
Co-fractionation Homo sapiens
19 TRIM52  
Affinity Capture-MS Homo sapiens
20 TSHR 7253
Affinity Capture-MS Homo sapiens
21 FOXB1  
Affinity Capture-MS Homo sapiens
22 RABIF  
Affinity Capture-MS Homo sapiens
23 EBNA-LP  
Affinity Capture-MS
24 MAGEA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 CCT8L2  
Affinity Capture-MS Homo sapiens
26 CUL3 8452
Affinity Capture-MS Homo sapiens
27 C9orf78 51759
Affinity Capture-MS Homo sapiens
28 SQSTM1 8878
Proximity Label-MS Homo sapiens
29 CBWD2  
Affinity Capture-MS Homo sapiens
30 CALM1 801
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 ARF6 382
Proximity Label-MS Homo sapiens
32 TXNDC12 51060
Co-fractionation Homo sapiens
33 SUCLA2 8803
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 MAGEA9  
Affinity Capture-MS Homo sapiens
35 C11orf52 91894
Proximity Label-MS Homo sapiens
36 LYN 4067
Proximity Label-MS Homo sapiens
37 CDC42 998
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
38 LRPPRC 10128
Co-fractionation Homo sapiens
39 POLR1A  
Co-fractionation Homo sapiens
40 RPA3 6119
Proximity Label-MS Homo sapiens
41 DTNBP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 ABTB2 25841
Affinity Capture-MS Homo sapiens
43 MYC  
Affinity Capture-MS Homo sapiens
44 BTF3 689
Affinity Capture-MS Homo sapiens
45 LMNA 4000
Cross-Linking-MS (XL-MS) Homo sapiens
46 ARRDC3 57561
Affinity Capture-MS Homo sapiens
47 RAC3 5881
Affinity Capture-MS Homo sapiens
48 PARP1 142
Proximity Label-MS Homo sapiens
49 PEX14 5195
Co-purification Homo sapiens
50 S100B 6285
Affinity Capture-MS Homo sapiens
51 ACTB 60
Proximity Label-MS Homo sapiens
52 KRAS 3845
Proximity Label-MS Homo sapiens
53 RHOB 388
Proximity Label-MS Homo sapiens
54 GNAI2 2771
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 RAC1 5879
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
56 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
57 MIER2  
Affinity Capture-MS Homo sapiens
58 ASIC2  
Affinity Capture-MS Homo sapiens
59 SDF4 51150
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 CBWD1 55871
Affinity Capture-MS Homo sapiens
61 CAV1 857
Proximity Label-MS Homo sapiens
62 EFCAB2  
Affinity Capture-MS Homo sapiens
63 CAMKK2 10645
Affinity Capture-MS Homo sapiens
64 Ksr1  
Affinity Capture-MS Mus musculus
65 MYL12B 103910
Affinity Capture-MS Homo sapiens
66 C9orf72  
Affinity Capture-MS Homo sapiens
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