Gene description for UBAC1
Gene name UBA domain containing 1
Gene symbol UBAC1
Other names/aliases GBDR1
UBADC1
Species Homo sapiens
 Database cross references - UBAC1
ExoCarta ExoCarta_10422
Vesiclepedia VP_10422
Entrez Gene 10422
HGNC 30221
MIM 608129
UniProt Q9BSL1  
 UBAC1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Urine 19056867    
 Gene ontology annotations for UBAC1
Molecular Function
    protein binding GO:0005515 IPI
    polyubiquitin modification-dependent protein binding GO:0031593 IDA
    proteasome binding GO:0070628 IDA
Biological Process
    protein ubiquitination GO:0016567 IDA
    protein ubiquitination GO:0016567 IEA
    protein maturation GO:0051604 IDA
Subcellular Localization
    ubiquitin ligase complex GO:0000151 IBA
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified UBAC1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for UBAC1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 A1BG 1
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 TWF2 11344
Co-fractionation Homo sapiens
3 PSMD6 9861
Affinity Capture-Western Homo sapiens
4 USP48  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 LINC01565  
Affinity Capture-MS Homo sapiens
6 RPL10 6134
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 UBC 7316
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
8 CSNK2A1 1457
Biochemical Activity Homo sapiens
9 RPS2P32  
Affinity Capture-MS Homo sapiens
10 KRTAP13-2  
Affinity Capture-MS Homo sapiens
11 APP 351
Reconstituted Complex Homo sapiens
12 TCEA1 6917
Co-fractionation Homo sapiens
13 HYAL1 3373
Affinity Capture-MS Homo sapiens
14 INSL3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 LECT2  
Affinity Capture-MS Homo sapiens
16 AKIRIN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 FBXW7  
Synthetic Lethality Homo sapiens
18 CCDC138  
Two-hybrid Homo sapiens
19 SLC27A2 11001
Affinity Capture-MS Homo sapiens
20 FTSJ2 29960
Affinity Capture-MS Homo sapiens
21 IGF2 3481
Affinity Capture-MS Homo sapiens
22 IGFL3  
Affinity Capture-MS Homo sapiens
23 MAP3K6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 DVL2 1856
Two-hybrid Homo sapiens
25 USP7 7874
Co-fractionation Homo sapiens
26 RNF123 63891
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 FBXW5  
Affinity Capture-MS Homo sapiens
28 RAD23A 5886
Affinity Capture-MS Homo sapiens
29 FAS 355
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
PCA Homo sapiens
Affinity Capture-Western Homo sapiens
PCA Homo sapiens
30 PRSS45  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 ZDHHC17 23390
Two-hybrid Homo sapiens
32 RDH12 145226
Affinity Capture-MS Homo sapiens
33 MED7  
Affinity Capture-MS Homo sapiens
34 CHIA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 PSMD4 5710
Affinity Capture-Western Homo sapiens
36 UBB 7314
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
37 UBE2D4  
Affinity Capture-MS Homo sapiens
38 HTT 3064
Two-hybrid Homo sapiens
39 DAZAP2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
40 PLEKHB2 55041
Two-hybrid Homo sapiens
41 BVES  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 POU6F2  
Two-hybrid Homo sapiens
43 CHMP4B 128866
Affinity Capture-MS Homo sapiens
44 MRPL51 51258
Affinity Capture-MS Homo sapiens
45 ALDH3B1 221
Affinity Capture-MS Homo sapiens
46 ARRDC3 57561
Two-hybrid Homo sapiens
47 GANAB 23193
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 INSL6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 EIF4EBP1  
Affinity Capture-MS Homo sapiens
50 MRPS14  
Affinity Capture-MS Homo sapiens
51 IQGAP2 10788
Affinity Capture-MS Homo sapiens
52 NMS  
Affinity Capture-MS Homo sapiens
53 MYH2  
Affinity Capture-MS Homo sapiens
54 TRIM2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
55 DEFB125  
Affinity Capture-MS Homo sapiens
56 C9orf24  
Affinity Capture-MS Homo sapiens
57 RELA 5970
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
58 UBQLN2 29978
Two-hybrid Homo sapiens
59 REG4 83998
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 BCAP31 10134
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 CUL3 8452
Affinity Capture-MS Homo sapiens
62 SPINK14  
Affinity Capture-MS Homo sapiens
63 MAP3K1 4214
Biochemical Activity Homo sapiens
64 GADD45GIP1  
Affinity Capture-MS Homo sapiens
65 ARHGEF16 27237
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 PSMD7 5713
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
67 RDH11 51109
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 ELAC2 60528
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 HRASLS2  
Two-hybrid Homo sapiens
70 GNLY  
Affinity Capture-MS Homo sapiens
71 CDKN1B 1027
Reconstituted Complex Homo sapiens
72 TRIM39  
Two-hybrid Homo sapiens
73 NEDD4L 23327
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
74 VPS25 84313
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
75 RNASE3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 RPS27A 6233
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 ITGA5 3678
Affinity Capture-MS Homo sapiens
78 AMBN  
Affinity Capture-MS Homo sapiens
79 FOXRED2  
Affinity Capture-MS Homo sapiens
80 NMB  
Affinity Capture-MS Homo sapiens
81 MAB21L1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 LCMT2 9836
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 PNPLA1 285848
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 SMURF1 57154
Biochemical Activity Homo sapiens
85 CCL5 6352
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 PLA2G5  
Affinity Capture-MS Homo sapiens
87 DDX58 23586
Affinity Capture-RNA Homo sapiens
88 WWP2 11060
Biochemical Activity Homo sapiens
89 AIRE  
Two-hybrid Homo sapiens
90 ZG16B 124220
Two-hybrid Homo sapiens
91 MGAT3  
Two-hybrid Homo sapiens
92 RTBDN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 SPG20 23111
Two-hybrid Homo sapiens
94 HSPA4L 22824
Co-fractionation Homo sapiens
95 IGFBP6 3489
Two-hybrid Homo sapiens
96 RSAD2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 UTP6  
Co-fractionation Homo sapiens
98 CPSF6 11052
Two-hybrid Homo sapiens
99 RPS6KA6 27330
Co-fractionation Homo sapiens
100 UNC119B 84747
Co-fractionation Homo sapiens
101 GREM2 64388
Affinity Capture-MS Homo sapiens
102 PARN  
Co-fractionation Homo sapiens
View the network image/svg+xml



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