Gene description for Dnmt1
Gene name DNA methyltransferase (cytosine-5) 1
Gene symbol Dnmt1
Other names/aliases Cxxc9
Dnmt
Dnmt1o
MCMT
MTase
Met-1
Met1
MommeD2
m.MmuI
Species Mus musculus
 Database cross references - Dnmt1
ExoCarta ExoCarta_13433
Vesiclepedia VP_13433
Entrez Gene 13433
UniProt P13864  
 Dnmt1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Dnmt1
Molecular Function
    zinc ion binding GO:0008270 IDA
    RNA binding GO:0003723 IDA
    chromatin binding GO:0003682 IDA
    protein domain specific binding GO:0019904 ISO
    transferase activity GO:0016740 IEA
    DNA binding GO:0003677 ISO
    histone deacetylase binding GO:0042826 ISO
    methyl-CpG binding GO:0008327 ISO
    DNA (cytosine-5-)-methyltransferase activity GO:0003886 ISO
    unmethylated CpG binding GO:0045322 ISO
    DNA-methyltransferase activity GO:0009008 ISO
    protein binding GO:0005515 IPI
    methyltransferase activity GO:0008168 IDA
    double-stranded DNA binding GO:0003690 ISO
    transcription factor binding GO:0008134 IEA
    estrogen receptor binding GO:0030331 ISO
    DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates GO:0051718 ISO
    metal ion binding GO:0046872 IEA
    catalytic activity GO:0003824 IEA
Biological Process
    regulation of transcription, DNA-templated GO:0006355 IEA
    positive regulation of histone H3-K4 methylation GO:0051571 ISO
    maintenance of DNA methylation GO:0010216 ISO
    positive regulation of gene expression GO:0010628 ISO
    negative regulation of transcription, DNA-templated GO:0045892 IMP
    metabolic process GO:0008152 IEA
    DNA methylation on cytosine within a CG sequence GO:0010424 ISO
    methylation GO:0032259 IEA
    chromatin modification GO:0016568 IEA
    regulation of gene expression GO:0010468 IMP
    cellular response to amino acid stimulus GO:0071230 IDA
    regulation of cell proliferation GO:0042127 IGI
    S-adenosylmethioninamine metabolic process GO:0046499 ISO
    cellular response to transforming growth factor beta stimulus GO:0071560 ISO
    negative regulation of histone H3-K9 methylation GO:0051573 ISO
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IMP
    response to antidepressant GO:0036276 ISO
    DNA hypermethylation GO:0044026 ISO
    gene silencing GO:0016458 IDA
    C-5 methylation of cytosine GO:0090116 ISO
    DNA methylation GO:0006306 IMP
    transcription, DNA-templated GO:0006351 IEA
    S-adenosylmethionine metabolic process GO:0046500 ISO
    S-adenosylhomocysteine metabolic process GO:0046498 ISO
    DNA methylation on cytosine GO:0032776 ISO
Subcellular Localization
    heterochromatin GO:0000792 IDA
    nucleus GO:0005634 ISO
    replication fork GO:0005657 IDA
    cytoplasm GO:0005737 ISO
    neuronal cell body GO:0043025 ISO
    pericentric heterochromatin GO:0005721 IDA
    protein complex GO:0043234 ISO
 Experiment description of studies that identified Dnmt1 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Dnmt1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HDAC1 3065
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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