Gene description for Trim28
Gene name tripartite motif-containing 28
Gene symbol Trim28
Other names/aliases AA408787
KAP-1
KRIP-1
MommeD9
Tif1b
Tif1beta
Species Mus musculus
 Database cross references - Trim28
ExoCarta ExoCarta_21849
Vesiclepedia VP_21849
Entrez Gene 21849
UniProt Q62318  
 Trim28 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Melanoma cells 34957415    
Melanoma cells 34957415    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Trim28
Molecular Function
    Krueppel-associated box domain binding GO:0035851 ISO
    tubulin-glutamic acid ligase activity GO:0070740 IEA
    poly(A) RNA binding GO:0044822 ISO
    ubiquitin protein ligase binding GO:0031625 ISO
    zinc ion binding GO:0008270 ISO
    protein-glycine ligase activity GO:0070735 IEA
    metal ion binding GO:0046872 IEA
    transcription corepressor activity GO:0003714 ISO
    protein-glycine ligase activity, initiating GO:0070736 IEA
    coenzyme F420-0 gamma-glutamyl ligase activity GO:0043773 IEA
    coenzyme F420-2 alpha-glutamyl ligase activity GO:0043774 IEA
    transcription coactivator activity GO:0003713 IDA
    sequence-specific DNA binding GO:0043565 IDA
    protein binding GO:0005515 IPI
    DNA binding GO:0003677 ISO
    ubiquitin-protein transferase activity GO:0004842 ISO
    tubulin-glycine ligase activity GO:0070738 IEA
    protein kinase activity GO:0004672 IDA
    protein-glycine ligase activity, elongating GO:0070737 IEA
    UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity GO:0008766 IEA
    protein-glutamic acid ligase activity GO:0070739 IEA
    ribosomal S6-glutamic acid ligase activity GO:0018169 IEA
    ligase activity GO:0016874 IEA
    sequence-specific DNA binding transcription factor activity GO:0003700 IDA
    chromo shadow domain binding GO:0070087 ISO
Biological Process
    protein ubiquitination GO:0016567 ISO
    DNA methylation involved in embryo development GO:0043045 IMP
    protein autophosphorylation GO:0046777 IDA
    negative regulation of DNA demethylation GO:1901536 IMP
    regulation of transcription, DNA-templated GO:0006355 IEA
    DNA repair GO:0006281 ISO
    positive regulation of transcription factor import into nucleus GO:0042993 ISO
    negative regulation of transcription, DNA-templated GO:0045892 ISO
    epithelial to mesenchymal transition GO:0001837 IDA
    positive regulation of transcription, DNA-templated GO:0045893 IDA
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IGI
    embryo implantation GO:0007566 IMP
    negative regulation of viral release from host cell GO:1902187 ISO
    protein oligomerization GO:0051259 ISO
    positive regulation of DNA repair GO:0045739 ISO
    embryonic placenta morphogenesis GO:0060669 IMP
    in utero embryonic development GO:0001701 IMP
    protein phosphorylation GO:0006468 IDA
    innate immune response GO:0045087 ISO
    convergent extension involved in axis elongation GO:0060028 IMP
    protein sumoylation GO:0016925 ISO
    regulation of genetic imprinting GO:2000653 IMP
    transcription, DNA-templated GO:0006351 IEA
Subcellular Localization
    RNA polymerase II transcription factor complex GO:0090575 IDA
    nucleoplasm GO:0005654 ISO
    nucleus GO:0005634 ISO
    nucleolus GO:0005730 IDA
    intracellular GO:0005622 IEA
    nuclear heterochromatin GO:0005720 IDA
    nuclear euchromatin GO:0005719 IDA
    chromatin GO:0000785 IDA
 Experiment description of studies that identified Trim28 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 1117
MISEV standards
✘ EV Biophysical techniques
✔
Cd81|Icam1
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H.
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 1118
MISEV standards
✘ EV Biophysical techniques
✔
Cd81|Icam1
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H.
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Trim28
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Trim28 is involved
PathwayEvidenceSource
Generic Transcription Pathway IEA Reactome





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