Gene description for Ppp1ca
Gene name protein phosphatase 1, catalytic subunit, alpha isoform
Gene symbol Ppp1ca
Other names/aliases Ppp1c
dism2
Species Mus musculus
 Database cross references - Ppp1ca
ExoCarta ExoCarta_19045
Vesiclepedia VP_19045
Entrez Gene 19045
UniProt P62137  
 Ppp1ca identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ppp1ca
Molecular Function
    hydrolase activity GO:0016787 IEA
    protein binding GO:0005515 IPI
    protein complex binding GO:0032403 ISO
    phosphatase activity GO:0016791 IDA
    phosphoprotein phosphatase activity GO:0004721 ISO
    metal ion binding GO:0046872 IEA
    ribonucleoprotein complex binding GO:0043021 ISO
    protein serine/threonine phosphatase activity GO:0004722 TAS
    protein phosphatase 1 binding GO:0008157 ISO
Biological Process
    cell division GO:0051301 IEA
    protein dephosphorylation GO:0006470 TAS
    branching morphogenesis of an epithelial tube GO:0048754 IMP
    regulation of translation GO:0006417 TAS
    female meiotic division GO:0007143 TAS
    dephosphorylation GO:0016311 ISS
    positive regulation of extrinsic apoptotic signaling pathway in absence of ligand GO:2001241 IMP
    cell cycle GO:0007049 IEA
    regulation of circadian rhythm GO:0042752 ISO
    cellular process GO:0009987 TAS
    rhythmic process GO:0048511 IEA
    regulation of glycogen catabolic process GO:0005981 ISO
    regulation of glycogen biosynthetic process GO:0005979 ISO
    lung development GO:0030324 IMP
    glycogen metabolic process GO:0005977 TAS
    circadian regulation of gene expression GO:0032922 IDA
    entrainment of circadian clock by photoperiod GO:0043153 IMP
    carbohydrate metabolic process GO:0005975 IEA
Subcellular Localization
    cytoplasm GO:0005737 ISO
    neuronal cell body GO:0043025 ISO
    nucleus GO:0005634 ISO
    MLL5-L complex GO:0070688 ISO
    PTW/PP1 phosphatase complex GO:0072357 ISO
    glycogen granule GO:0042587 ISO
    nucleoplasm GO:0005654 ISO
    neuron projection GO:0043005 ISO
    perikaryon GO:0043204 ISO
    dendritic spine GO:0043197 ISO
    protein phosphatase type 1 complex GO:0000164 ISO
    extracellular exosome GO:0070062 ISO
    plasma membrane GO:0005886 ISO
 Experiment description of studies that identified Ppp1ca in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD81|FLOT1
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 15
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ppp1ca
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TGFBR1 7046
Affinity Capture-Luminescence Homo sapiens
View the network image/svg+xml



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