Gene description for AP1S1
Gene name adaptor-related protein complex 1, sigma 1 subunit
Gene symbol AP1S1
Other names/aliases AP19
CLAPS1
EKV3
MEDNIK
SIGMA1A
Species Homo sapiens
 Database cross references - AP1S1
ExoCarta ExoCarta_1174
Vesiclepedia VP_1174
Entrez Gene 1174
HGNC 559
MIM 603531
UniProt P61966  
 AP1S1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for AP1S1
Molecular Function
    protein binding GO:0005515 IPI
    clathrin adaptor activity GO:0035615 IEA
Biological Process
    intracellular protein transport GO:0006886 IEA
    receptor-mediated endocytosis GO:0006898 TAS
    response to virus GO:0009615 IEP
    vesicle-mediated transport GO:0016192 IBA
    vesicle-mediated transport GO:0016192 NAS
    platelet dense granule organization GO:0060155 NAS
    basolateral protein secretion GO:0110010 NAS
    melanosome assembly GO:1903232 NAS
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    lysosomal membrane GO:0005765 NAS
    lysosomal membrane GO:0005765 TAS
    early endosome GO:0005769 NAS
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 TAS
    clathrin-coated pit GO:0005905 IEA
    membrane GO:0016020 HDA
    AP-1 adaptor complex GO:0030121 NAS
    AP-1 adaptor complex GO:0030121 TAS
    cytoplasmic vesicle membrane GO:0030659 TAS
    trans-Golgi network membrane GO:0032588 NAS
    trans-Golgi network membrane GO:0032588 TAS
    intracellular membrane-bounded organelle GO:0043231 IBA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified AP1S1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for AP1S1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SLC16A1 6566
Affinity Capture-MS Homo sapiens
2 DDX6 1656
Co-fractionation Homo sapiens
3 FTL 2512
Affinity Capture-MS Homo sapiens
4 GTSE1 51512
Affinity Capture-MS Homo sapiens
5 AP2B1 163
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
6 RHOU 58480
Reconstituted Complex Homo sapiens
7 CANX 821
Affinity Capture-MS Homo sapiens
8 STAT1 6772
Co-fractionation Homo sapiens
9 XPO1 7514
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
10 E2F4  
Affinity Capture-MS Homo sapiens
11 ATG9A 79065
Proximity Label-MS Homo sapiens
12 UBA1 7317
Co-fractionation Homo sapiens
13 AP1M1 8907
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
14 EGFR 1956
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 CAP2 10486
Co-fractionation Homo sapiens
16 FTH1 2495
Co-fractionation Homo sapiens
17 DDX58 23586
Affinity Capture-RNA Homo sapiens
18 AP1M2 10053
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 AP1G2 8906
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
20 AP2M1 1173
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 ARMC6 93436
Affinity Capture-MS Homo sapiens
22 MRFAP1L1  
Affinity Capture-MS Homo sapiens
23 EBLN2  
Affinity Capture-MS Homo sapiens
24 MOV10 4343
Affinity Capture-RNA Homo sapiens
25 PRDX3 10935
Co-fractionation Homo sapiens
26 SEC31A 22872
Co-fractionation Homo sapiens
27 SPATA33  
Affinity Capture-MS Homo sapiens
28 CLTA 1211
Affinity Capture-MS Homo sapiens
29 AP1B1 162
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
30 EEF1E1 9521
Co-fractionation Homo sapiens
31 SSUH2  
Affinity Capture-MS Homo sapiens
32 TCEB2 6923
Co-fractionation Homo sapiens
33 AP1G1 164
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
34 RAB10 10890
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 RPA2 6118
Proximity Label-MS Homo sapiens
36 RPA3 6119
Proximity Label-MS Homo sapiens
37 RCC1 1104
Co-fractionation Homo sapiens
38 COMT 1312
Affinity Capture-MS Homo sapiens
39 SEC61A1 29927
Affinity Capture-MS Homo sapiens
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