Gene description for PAPSS2
Gene name 3'-phosphoadenosine 5'-phosphosulfate synthase 2
Gene symbol PAPSS2
Other names/aliases ATPSK2
BCYM4
SK2
Species Homo sapiens
 Database cross references - PAPSS2
ExoCarta ExoCarta_9060
Entrez Gene 9060
HGNC 8604
MIM 603005
UniProt O95340  
 PAPSS2 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Thymus 23844026    
 Gene ontology annotations for PAPSS2
Molecular Function
    ATP binding GO:0005524 IEA
    nucleotidyltransferase activity GO:0016779 ISS
    sulfate adenylyltransferase (ATP) activity GO:0004781 ISS
    adenylylsulfate kinase activity GO:0004020 IBA
Biological Process
    carbohydrate metabolic process GO:0005975 TAS
    bone development GO:0060348 IEA
    3'-phosphoadenosine 5'-phosphosulfate biosynthetic process GO:0050428 TAS
    small molecule metabolic process GO:0044281 TAS
    3'-phosphoadenosine 5'-phosphosulfate metabolic process GO:0050427 TAS
    phosphorylation GO:0016310 IEA
    glycosaminoglycan metabolic process GO:0030203 TAS
    blood coagulation GO:0007596 IEA
    sulfate assimilation GO:0000103 IEA
    skeletal system development GO:0001501 TAS
    xenobiotic metabolic process GO:0006805 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
 Experiment description of studies that identified PAPSS2 in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PAPSS2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 VHL  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PAPSS2 is involved
PathwayEvidenceSource
Defective PAPSS2 causes SEMD-PA TAS Reactome
Transport and synthesis of PAPS TAS Reactome
Transport and synthesis of PAPS TAS Reactome





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