Gene description for Nsf
Gene name N-ethylmaleimide sensitive fusion protein
Gene symbol Nsf
Other names/aliases AI316878
AU020090
AU067812
SKD2
Species Mus musculus
 Database cross references - Nsf
ExoCarta ExoCarta_18195
Vesiclepedia VP_18195
Entrez Gene 18195
UniProt P46460  
 Nsf identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Nsf
Molecular Function
    D1 dopamine receptor binding GO:0031748 ISO
    PDZ domain binding GO:0030165 ISO
    ATPase activity GO:0016887 ISO
    metal ion binding GO:0046872 IEA
    ATPase activity, coupled GO:0042623 IMP
    protein kinase binding GO:0019901 IPI
    syntaxin-1 binding GO:0017075 IDA
    protein complex binding GO:0032403 ISO
    protein binding GO:0005515 IPI
    Rab GTPase binding GO:0017137 ISO
    nucleotide binding GO:0000166 IEA
    hydrolase activity GO:0016787 IEA
    ATP binding GO:0005524 IEA
    syntaxin binding GO:0019905 IDA
    protein C-terminus binding GO:0008022 ISO
    ATP-dependent protein binding GO:0043008 ISO
Biological Process
    intracellular protein transport GO:0006886 ISO
    metabolic process GO:0008152 ISO
    vesicle-mediated transport GO:0016192 ISO
    potassium ion transport GO:0006813 IDA
    Golgi vesicle docking GO:0048211 IBA
    transport GO:0006810 IEA
    positive regulation of receptor recycling GO:0001921 ISO
    protein transport GO:0015031 ISO
    Golgi to plasma membrane protein transport GO:0043001 IBA
    positive regulation of protein catabolic process GO:0045732 ISO
    intra-Golgi vesicle-mediated transport GO:0006891 IBA
    regulation of exocytosis GO:0017157 ISO
Subcellular Localization
    postsynaptic density GO:0014069 ISO
    extracellular exosome GO:0070062 ISO
    plasma membrane GO:0005886 ISO
    lysosomal membrane GO:0005765 ISO
    dendritic shaft GO:0043198 ISO
    Golgi stack GO:0005795 ISO
    myelin sheath GO:0043209 IDA
    cytoplasm GO:0005737 ISO
 Experiment description of studies that identified Nsf in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Nsf
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Nsf is involved
PathwayEvidenceSource
Trafficking of GluR2-containing AMPA receptors IEA Reactome





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