Gene description for Ube2o
Gene name ubiquitin-conjugating enzyme E2O
Gene symbol Ube2o
Other names/aliases 9630022H21
B230113M03Rik
E2-230K
mKIAA1734
Species Mus musculus
 Database cross references - Ube2o
ExoCarta ExoCarta_217342
Vesiclepedia VP_217342
Entrez Gene 217342
UniProt Q6ZPJ3  
 Ube2o identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ube2o
Molecular Function
    poly(A) RNA binding GO:0044822 ISO
    UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity GO:0008766 IEA
    coenzyme F420-0 gamma-glutamyl ligase activity GO:0043773 IEA
    protein-glycine ligase activity, initiating GO:0070736 IEA
    ligase activity GO:0016874 IEA
    ubiquitin-protein transferase activity GO:0004842 ISO
    protein-glutamic acid ligase activity GO:0070739 IEA
    ubiquitin protein ligase binding GO:0031625 IBA
    coenzyme F420-2 alpha-glutamyl ligase activity GO:0043774 IEA
    protein-glycine ligase activity GO:0070735 IEA
    ubiquitin conjugating enzyme activity GO:0061631 ISO
    tubulin-glycine ligase activity GO:0070738 IEA
    protein-glycine ligase activity, elongating GO:0070737 IEA
    ubiquitin protein ligase activity GO:0061630 ISO
    ribosomal S6-glutamic acid ligase activity GO:0018169 IEA
    tubulin-glutamic acid ligase activity GO:0070740 IEA
    ATP binding GO:0005524 IEA
    nucleotide binding GO:0000166 IEA
Biological Process
    protein monoubiquitination GO:0006513 ISO
    positive regulation of BMP signaling pathway GO:0030513 ISO
    protein K63-linked ubiquitination GO:0070534 ISO
    protein polyubiquitination GO:0000209 IBA
    retrograde transport, endosome to Golgi GO:0042147 ISO
Subcellular Localization
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
    nucleus GO:0005634 ISO
 Experiment description of studies that identified Ube2o in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ube2o
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CPNE1 8904
Reconstituted Complex Homo sapiens
2 CPNE4 131034
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which Ube2o is involved
No pathways found





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