Gene description for Dync1h1
Gene name dynein cytoplasmic 1 heavy chain 1
Gene symbol Dync1h1
Other names/aliases 9930018I23Rik
AI894280
DHC1
DHC1a
DNCL
Dnchc1
Dnec1
Dnecl
Loa
MAP1C
P22
Swl
mKIAA0325
Species Mus musculus
 Database cross references - Dync1h1
ExoCarta ExoCarta_13424
Vesiclepedia VP_13424
Entrez Gene 13424
UniProt Q9JHU4  
 Dync1h1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Dync1h1
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    minus-end-directed microtubule motor activity GO:0008569 IBA
    minus-end-directed microtubule motor activity GO:0008569 IEA
    ATP hydrolysis activity GO:0016887 IEA
    dynein intermediate chain binding GO:0045505 IBA
    dynein intermediate chain binding GO:0045505 IEA
    dynein light intermediate chain binding GO:0051959 IBA
    dynein light intermediate chain binding GO:0051959 IPI
    dynein light intermediate chain binding GO:0051959 ISO
Biological Process
    cilium movement GO:0003341 IC
    mitotic spindle organization GO:0007052 IBA
    nuclear migration GO:0007097 IBA
    retrograde axonal transport GO:0008090 IBA
    cytoplasmic microtubule organization GO:0031122 IBA
    positive regulation of intracellular transport GO:0032388 ISO
    positive regulation of intracellular transport GO:0032388 ISS
    P-body assembly GO:0033962 IMP
    stress granule assembly GO:0034063 IMP
    establishment of spindle localization GO:0051293 IEA
    establishment of spindle localization GO:0051293 ISO
    cell division GO:0051301 IEA
    regulation of mitotic spindle organization GO:0060236 ISO
    regulation of mitotic spindle organization GO:0060236 ISS
    regulation of metaphase plate congression GO:0090235 ISO
    regulation of metaphase plate congression GO:0090235 ISS
    positive regulation of cold-induced thermogenesis GO:0120162 IMP
    positive regulation of spindle assembly GO:1905832 ISO
    positive regulation of spindle assembly GO:1905832 ISS
Subcellular Localization
    manchette GO:0002177 ISO
    nuclear envelope GO:0005635 ISO
    cytoplasm GO:0005737 IDA
    centrosome GO:0005813 IEA
    centrosome GO:0005813 ISO
    cytoplasmic dynein complex GO:0005868 IBA
    cytoplasmic dynein complex GO:0005868 ISM
    cytoplasmic dynein complex GO:0005868 ISO
    microtubule GO:0005874 IEA
    microtubule GO:0005874 ISO
    cytoplasmic microtubule GO:0005881 IBA
    cell cortex GO:0005938 IBA
    filopodium GO:0030175 IDA
    dynein complex GO:0030286 ISO
    axon GO:0030424 ISO
    neuronal cell body GO:0043025 ISO
 Experiment description of studies that identified Dync1h1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Dync1h1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tnik  
Affinity Capture-MS Mus musculus
2 Unk  
Affinity Capture-RNA Mus musculus
3 Pde4dip  
Affinity Capture-MS Mus musculus
4 Bicd2  
Co-fractionation Mus musculus
5 Pou5f1  
Affinity Capture-MS Mus musculus
6 DYNLT1 6993
Affinity Capture-MS Homo sapiens
7 FOLR1 2348
Affinity Capture-MS Homo sapiens
8 Katnb1  
Co-fractionation Mus musculus
9 DYNC1I2 1781
Affinity Capture-MS Homo sapiens
10 Syngap1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
11 NSUN2 54888
Affinity Capture-MS Homo sapiens
12 Fmr1  
Affinity Capture-MS Mus musculus
13 Foxp3  
Affinity Capture-MS Mus musculus
14 Kctd13  
Affinity Capture-MS Mus musculus
15 Shank3  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
16 KATNA1 11104
Two-hybrid Homo sapiens
17 Ywhaz 22631
Affinity Capture-MS Mus musculus
18 Ndel1  
Co-purification Mus musculus
Co-fractionation Mus musculus
19 DYNC1H1 1778
Affinity Capture-MS Homo sapiens
20 HTT 3064
Affinity Capture-MS Homo sapiens
21 Mycbp2 105689
Affinity Capture-MS Mus musculus
22 Cyfip1 20430
Affinity Capture-MS Mus musculus
23 PPP1R12A 4659
Affinity Capture-MS Homo sapiens
24 Dync1h1 13424
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
25 Agap2  
Affinity Capture-MS Mus musculus
26 DYNC1LI1 51143
Affinity Capture-MS Homo sapiens
27 DYNC1LI2 1783
Affinity Capture-MS Homo sapiens
28 Pafah1b1 18472
Affinity Capture-Western Mus musculus
29 Tpr 108989
Co-fractionation Mus musculus
30 DYNLRB1 83658
Affinity Capture-MS Homo sapiens
31 Nudc 18221
Affinity Capture-Western Mus musculus
32 Eed  
Affinity Capture-MS Mus musculus
33 Atxn1  
Affinity Capture-MS Mus musculus
34 DYNLL1 8655
Affinity Capture-MS Homo sapiens
35 Dync1i1  
Affinity Capture-Western Mus musculus
36 Fancd2  
Affinity Capture-MS Mus musculus
37 KATNB1  
Two-hybrid Homo sapiens
38 DYNLT3 6990
Affinity Capture-MS Homo sapiens
39 Kif3b  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Dync1h1 is involved
PathwayEvidenceSource
Adaptive Immune System IEA Reactome
Aggrephagy IEA Reactome
Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal IEA Reactome
Amplification of signal from the kinetochores IEA Reactome
Anchoring of the basal body to the plasma membrane IEA Reactome
Asparagine N-linked glycosylation IEA Reactome
AURKA Activation by TPX2 IEA Reactome
Autophagy IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle Checkpoints IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
Centrosome maturation IEA Reactome
Cilium Assembly IEA Reactome
COPI-independent Golgi-to-ER retrograde traffic IEA Reactome
COPI-mediated anterograde transport IEA Reactome
EML4 and NUDC in mitotic spindle formation IEA Reactome
ER to Golgi Anterograde Transport IEA Reactome
G2/M Transition IEA Reactome
Golgi-to-ER retrograde transport IEA Reactome
HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand IEA Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome
Intra-Golgi and retrograde Golgi-to-ER traffic IEA Reactome
Loss of Nlp from mitotic centrosomes IEA Reactome
Loss of proteins required for interphase microtubule organization from the centrosome IEA Reactome
M Phase IEA Reactome
Macroautophagy IEA Reactome
Membrane Trafficking IEA Reactome
Metabolism of proteins IEA Reactome
MHC class II antigen presentation IEA Reactome
Mitotic Anaphase IEA Reactome
Mitotic G2-G2/M phases IEA Reactome
Mitotic Metaphase and Anaphase IEA Reactome
Mitotic Prometaphase IEA Reactome
Mitotic Spindle Checkpoint IEA Reactome
Neutrophil degranulation IEA Reactome
Organelle biogenesis and maintenance IEA Reactome
Post-translational protein modification IEA Reactome
Recruitment of mitotic centrosome proteins and complexes IEA Reactome
Recruitment of NuMA to mitotic centrosomes IEA Reactome
Regulation of PLK1 Activity at G2/M Transition IEA Reactome
Resolution of Sister Chromatid Cohesion IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases Activate Formins IEA Reactome
Selective autophagy IEA Reactome
Separation of Sister Chromatids IEA Reactome
Signal Transduction IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
Transport to the Golgi and subsequent modification IEA Reactome
Vesicle-mediated transport IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here