Gene description for Nefm
Gene name neurofilament, medium polypeptide
Gene symbol Nefm
Other names/aliases NF-M
NF160
NF165
Nef3
Nfm
Species Mus musculus
 Database cross references - Nefm
ExoCarta ExoCarta_18040
Entrez Gene 18040
UniProt P08553  
 Nefm identified in exosomes derived from the following tissue/cell type
Pancreatic cells 19351151    
 Gene ontology annotations for Nefm
Molecular Function
    structural molecule activity GO:0005198 IEA
    toxic substance binding GO:0015643 ISO
    protein heterodimerization activity GO:0046982 ISO
    receptor binding GO:0005102 ISO
Biological Process
    neurofilament bundle assembly GO:0033693 ISO
    intermediate filament polymerization or depolymerization GO:0045105 ISO
    neurofilament cytoskeleton organization GO:0060052 IMP
    intermediate filament cytoskeleton organization GO:0045104 IMP
    intermediate filament bundle assembly GO:0045110 IGI
    regulation of axon diameter GO:0031133 IMP
    axon cargo transport GO:0008088 IMP
    microtubule cytoskeleton organization GO:0000226 IGI
Subcellular Localization
    neurofilament GO:0005883 ISO
    neuron projection GO:0043005 IDA
    axon GO:0030424 ISO
    intermediate filament GO:0005882 ISO
    neuromuscular junction GO:0031594 IDA
    cytoskeleton GO:0005856 ISO
    intracellular GO:0005622 IMP
    cell GO:0005623 IMP
    perikaryon GO:0043204 ISO
    neuronal postsynaptic density GO:0097481 IDA
    myelin sheath GO:0043209 IDA
 Experiment description of studies that identified Nefm in exosomes
1
Experiment ID 188
ISEV standards
EM
EV Biophysical techniques
GAPDH|UCHL1|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Nefm
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Nefm is involved
No pathways found





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