Gene description for Glul
Gene name glutamate-ammonia ligase (glutamine synthetase)
Gene symbol Glul
Other names/aliases GS
Glns
Species Mus musculus
 Database cross references - Glul
ExoCarta ExoCarta_14645
Entrez Gene 14645
UniProt P15105  
 Glul identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Glul
Molecular Function
    magnesium ion binding GO:0000287 ISO
    lyase activity GO:0016829 IEA
    protein binding GO:0005515 IPI
    catalytic activity GO:0003824 IEA
    glutamate binding GO:0016595 ISO
    ligase activity GO:0016874 IEA
    identical protein binding GO:0042802 ISO
    dynein light chain binding GO:0045503 ISO
    manganese ion binding GO:0030145 ISO
    ATP binding GO:0005524 ISO
    nucleotide binding GO:0000166 IEA
    glutamate decarboxylase activity GO:0004351 IEA
    glutamate-ammonia ligase activity GO:0004356 ISO
Biological Process
    protein homooligomerization GO:0051260 ISO
    glutamate metabolic process GO:0006536 ISO
    positive regulation of insulin secretion GO:0032024 ISO
    cellular response to starvation GO:0009267 IEP
    positive regulation of synaptic transmission, glutamatergic GO:0051968 ISO
    response to glucose GO:0009749 IMP
    cell proliferation GO:0008283 ISO
    glutamine biosynthetic process GO:0006542 ISO
    positive regulation of epithelial cell proliferation GO:0050679 ISO
    ammonia assimilation cycle GO:0019676 ISO
    nitrogen compound metabolic process GO:0006807 IEA
Subcellular Localization
    perikaryon GO:0043204 ISO
    cytoplasm GO:0005737 ISO
    neuron projection GO:0043005 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    rough endoplasmic reticulum GO:0005791 ISO
    glial cell projection GO:0097386 IDA
    cell projection GO:0042995 ISO
    mitochondrion GO:0005739 IDA
    myelin sheath GO:0043209 ISO
    cell body GO:0044297 IDA
    protein complex GO:0043234 ISO
    nucleus GO:0005634 ISO
    extracellular exosome GO:0070062 ISO
    axon terminus GO:0043679 ISO
 Experiment description of studies that identified Glul in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Glul
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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