Gene description for Sptbn1
Gene name spectrin beta, non-erythrocytic 1
Gene symbol Sptbn1
Other names/aliases 9930031C03Rik
AL033301
SPTB2
Spnb-2
Spnb2
elf1
elf3
mKIAA4049
Species Mus musculus
 Database cross references - Sptbn1
ExoCarta ExoCarta_20742
Vesiclepedia VP_20742
Entrez Gene 20742
UniProt Q62261  
 Sptbn1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Sptbn1
Molecular Function
    actin binding GO:0003779 IEA
    structural constituent of cytoskeleton GO:0005200 ISO
    structural constituent of cytoskeleton GO:0005200 ISS
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IEA
    phospholipid binding GO:0005543 IEA
    ankyrin binding GO:0030506 IEA
    ankyrin binding GO:0030506 ISO
    protein-containing complex binding GO:0044877 IEA
    protein-containing complex binding GO:0044877 ISO
    actin filament binding GO:0051015 IBA
    GTPase binding GO:0051020 IEA
    GTPase binding GO:0051020 ISO
Biological Process
    mitotic cytokinesis GO:0000281 IEA
    mitotic cytokinesis GO:0000281 ISO
    plasma membrane organization GO:0007009 IEA
    plasma membrane organization GO:0007009 ISO
    central nervous system development GO:0007417 IMP
    central nervous system development GO:0007417 ISO
    central nervous system formation GO:0021556 IMP
    central nervous system formation GO:0021556 ISO
    actin cytoskeleton organization GO:0030036 IBA
    actin cytoskeleton organization GO:0030036 ISO
    actin cytoskeleton organization GO:0030036 ISS
    positive regulation of interleukin-2 production GO:0032743 IEA
    positive regulation of interleukin-2 production GO:0032743 ISO
    Golgi to plasma membrane protein transport GO:0043001 IEA
    Golgi to plasma membrane protein transport GO:0043001 ISO
    actin filament capping GO:0051693 IEA
    regulation of SMAD protein signal transduction GO:0060390 IDA
    membrane assembly GO:0071709 IEA
    membrane assembly GO:0071709 ISO
    protein localization to plasma membrane GO:0072659 IBA
    protein localization to plasma membrane GO:0072659 IMP
    protein localization to plasma membrane GO:0072659 ISO
    regulation of protein localization to plasma membrane GO:1903076 ISO
    positive regulation of protein localization to plasma membrane GO:1903078 IEA
    positive regulation of protein localization to plasma membrane GO:1903078 ISO
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    spectrin GO:0008091 IEA
    endomembrane system GO:0012505 IEA
    postsynaptic density GO:0014069 IDA
    membrane GO:0016020 IDA
    cell junction GO:0030054 IBA
    axolemma GO:0030673 IDA
    cortical cytoskeleton GO:0030863 IDA
    cortical actin cytoskeleton GO:0030864 IBA
    M band GO:0031430 IDA
    cuticular plate GO:0032437 IDA
    protein-containing complex GO:0032991 ISO
    cell projection GO:0042995 IBA
    postsynapse GO:0098794 ISO
    glutamatergic synapse GO:0098978 IEA
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Sptbn1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Sptbn1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kctd13  
Affinity Capture-MS Mus musculus
2 Tnik  
Affinity Capture-MS Mus musculus
3 SNCA 6622
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
4 Hcn1  
Affinity Capture-MS Mus musculus
5 Cnksr2  
Affinity Capture-MS Mus musculus
6 Cyfip2 76884
Affinity Capture-MS Mus musculus
7 Mycbp2 105689
Affinity Capture-MS Mus musculus
8 Kalrn  
Affinity Capture-MS Mus musculus
9 Hspb2 69253
Affinity Capture-MS Mus musculus
10 Dlgap1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
11 Syngap1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
12 Fmr1  
Affinity Capture-MS Mus musculus
13 Cebpa  
Affinity Capture-MS Mus musculus
14 Dlg4  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
15 Shank3  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
16 Pex5l  
Affinity Capture-MS Mus musculus
17 Iqcb1  
Affinity Capture-MS Mus musculus
18 Ywhaz 22631
Affinity Capture-MS Mus musculus
19 Sptbn1 20742
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
20 Nckap1 50884
Affinity Capture-MS Mus musculus
21 Herc2  
Affinity Capture-MS Mus musculus
22 Nphp4  
Affinity Capture-MS Mus musculus
23 Pde4dip  
Affinity Capture-MS Mus musculus
24 Cyfip1 20430
Affinity Capture-MS Mus musculus
25 Agap2  
Affinity Capture-MS Mus musculus
26 Unk  
Affinity Capture-RNA Mus musculus
27 Camsap1  
Co-fractionation Mus musculus
28 Huwe1  
Affinity Capture-MS Mus musculus
29 Sstr3  
Proximity Label-MS Mus musculus
30 CPNE1 8904
Reconstituted Complex Homo sapiens
31 Eed  
Affinity Capture-MS Mus musculus
32 Ctnna1 12385
Co-fractionation Mus musculus
33 Fancd2  
Affinity Capture-MS Mus musculus
34 Homer1  
Affinity Capture-MS Mus musculus
35 CPNE4 131034
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml



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