Gene description for Capza2
Gene name capping protein (actin filament) muscle Z-line, alpha 2
Gene symbol Capza2
Other names/aliases 1110053K06Rik
AW208808
Cappa2
Species Mus musculus
 Database cross references - Capza2
ExoCarta ExoCarta_12343
Vesiclepedia VP_12343
Entrez Gene 12343
UniProt P47754  
 Capza2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Capza2
Molecular Function
    actin binding GO:0003779 IEA
    protein binding GO:0005515 IPI
    actin filament binding GO:0051015 IBA
Biological Process
    actin cytoskeleton organization GO:0030036 IBA
    barbed-end actin filament capping GO:0051016 IBA
    barbed-end actin filament capping GO:0051016 IEA
Subcellular Localization
    cytosol GO:0005829 TAS
    brush border GO:0005903 IDA
    F-actin capping protein complex GO:0008290 IBA
    F-actin capping protein complex GO:0008290 IPI
    membrane GO:0016020 IDA
    cortical cytoskeleton GO:0030863 IBA
    cortical cytoskeleton GO:0030863 IDA
 Experiment description of studies that identified Capza2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Capza2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Psmg1 56088
Co-fractionation Mus musculus
2 SNCA 6622
Affinity Capture-MS Homo sapiens
3 Strap 20901
Co-fractionation Mus musculus
4 ABCG1  
Affinity Capture-MS Homo sapiens
5 Dlgap1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
6 Atxn1  
Affinity Capture-MS Mus musculus
7 Kctd13  
Affinity Capture-MS Mus musculus
8 Dlg4  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
9 Shank3  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
10 Twf2  
Co-fractionation Mus musculus
11 Lcp1 18826
Co-fractionation Mus musculus
12 Rpl18a 76808
Co-fractionation Mus musculus
13 Rcsd1  
Reconstituted Complex Mus musculus
14 Ywhae 22627
Affinity Capture-MS Mus musculus
15 Mettl21e  
Affinity Capture-MS Mus musculus
16 Cyfip1 20430
Affinity Capture-MS Mus musculus
17 Sh3kbp1 58194
Co-fractionation Mus musculus
18 Nanog  
Affinity Capture-MS Mus musculus
19 HACE1 57531
Affinity Capture-MS Homo sapiens
20 Lmo2  
Two-hybrid Mus musculus
21 Eed  
Affinity Capture-MS Mus musculus
22 Ywhaz 22631
Affinity Capture-MS Mus musculus
23 Mapt  
Affinity Capture-MS Mus musculus
24 Capzb 12345
Cross-Linking-MS (XL-MS) Mus musculus
25 Fancd2  
Affinity Capture-MS Mus musculus
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