Gene description for Sh3kbp1
Gene name SH3-domain kinase binding protein 1
Gene symbol Sh3kbp1
Other names/aliases 1200007H22Rik
1700125L08Rik
5830464D22Rik
AI447724
IN85
Ruk
Seta
Species Mus musculus
 Database cross references - Sh3kbp1
ExoCarta ExoCarta_58194
Entrez Gene 58194
UniProt Q8R550  
 Sh3kbp1 identified in exosomes derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for Sh3kbp1
Molecular Function
    SH3 domain binding GO:0017124 IEA
    protein binding GO:0005515 IPI
Biological Process
    regulation of cell shape GO:0008360 ISO
    cell migration GO:0016477 ISO
    cytoskeleton organization GO:0007010 ISO
    apoptotic process GO:0006915 IEA
    endocytosis GO:0006897 IEA
Subcellular Localization
    cell junction GO:0030054 IEA
    cell-cell junction GO:0005911 IDA
    cytoplasmic vesicle GO:0031410 IEA
    cytoplasm GO:0005737 IEA
    cytoskeleton GO:0005856 IEA
    synapse GO:0045202 IEA
    membrane GO:0016020 IEA
    neuron projection GO:0043005 IEA
 Experiment description of studies that identified Sh3kbp1 in exosomes
1
Experiment ID 214
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Sh3kbp1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BMPR1B  
Affinity Capture-Luminescence Homo sapiens
View the network image/svg+xml



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