Gene description for Rab5a
Gene name RAB5A, member RAS oncogene family
Gene symbol Rab5a
Other names/aliases 2410015H04Rik
AI663973
AU021172
nnyRab5a
Species Mus musculus
 Database cross references - Rab5a
ExoCarta ExoCarta_271457
Vesiclepedia VP_271457
Entrez Gene 271457
UniProt Q9CQD1  
 Rab5a identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
Mov neuroglial cells 15210972    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rab5a
Molecular Function
    nucleotide binding GO:0000166 IEA
    GDP binding GO:0019003 ISO
    GDP-dissociation inhibitor binding GO:0051021 ISO
    protein binding GO:0005515 IPI
    GTPase activity GO:0003924 TAS
    guanyl nucleotide binding GO:0019001 IDA
    GTP binding GO:0005525 ISO
Biological Process
    regulation of endosome size GO:0051036 ISO
    regulation of filopodium assembly GO:0051489 ISO
    positive regulation of exocytosis GO:0045921 ISO
    regulation of synaptic vesicle exocytosis GO:2000300 ISO
    regulation of endocytosis GO:0030100 IBA
    protein transport GO:0015031 IEA
    transport GO:0006810 IEA
    regulation of long-term neuronal synaptic plasticity GO:0048169 ISO
    positive regulation of smooth muscle cell proliferation GO:0048661 ISO
    small GTPase mediated signal transduction GO:0007264 IEA
    positive regulation of smooth muscle cell migration GO:0014911 ISO
    endosome organization GO:0007032 ISO
    Rab protein signal transduction GO:0032482 IBA
    intracellular protein transport GO:0006886 IBA
    early endosome to late endosome transport GO:0045022 ISO
    receptor internalization involved in canonical Wnt signaling pathway GO:2000286 ISO
    synaptic vesicle recycling GO:0036465 ISO
    endocytosis GO:0006897 ISO
Subcellular Localization
    cytoplasm GO:0005737 ISO
    cell projection GO:0042995 IEA
    cytoplasmic side of early endosome membrane GO:0098559 ISO
    dendrite GO:0030425 ISO
    membrane GO:0016020 IEA
    cell GO:0005623 IBA
    synaptic vesicle GO:0008021 ISO
    cytoplasmic vesicle GO:0031410 IEA
    extracellular exosome GO:0070062 ISO
    terminal bouton GO:0043195 ISO
    membrane raft GO:0045121 IDA
    zymogen granule membrane GO:0042589 ISO
    cytosol GO:0005829 ISO
    endosome GO:0005768 ISO
    actin cytoskeleton GO:0015629 IDA
    phagocytic vesicle GO:0045335 IDA
    axon GO:0030424 ISO
    plasma membrane GO:0005886 ISO
    somatodendritic compartment GO:0036477 ISO
    neuronal cell body GO:0043025 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    protein complex GO:0043234 ISO
    early endosome GO:0005769 ISO
    axon terminus GO:0043679 ISO
    endocytic vesicle GO:0030139 IDA
    ruffle GO:0001726 IDA
    intracellular GO:0005622 IBA
    neuron projection GO:0043005 ISO
    recycling endosome GO:0055037 ISO
 Experiment description of studies that identified Rab5a in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 39
MISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|HSC70|FLOT1
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
4
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rab5a
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Rab5a is involved
PathwayEvidenceSource
Factors involved in megakaryocyte development and platelet production IEA Reactome





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